GR61A_DROME
ID GR61A_DROME Reviewed; 436 AA.
AC Q9W0M2;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Gustatory receptor for sugar taste 61a;
GN Name=Gr61a; ORFNames=CG13888;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF47421.2};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP PREDICTION OF FUNCTION.
RX PubMed=14608037; DOI=10.1073/pnas.2335847100;
RA Robertson H.M., Warr C.G., Carlson J.R.;
RT "Molecular evolution of the insect chemoreceptor gene superfamily in
RT Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14537-14542(2003).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17715294; DOI=10.1073/pnas.0702421104;
RA Jiao Y., Moon S.J., Montell C.;
RT "A Drosophila gustatory receptor required for the responses to sucrose,
RT glucose, and maltose identified by mRNA tagging.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:14110-14115(2007).
RN [6]
RP FUNCTION.
RX PubMed=23850280; DOI=10.1016/j.cub.2013.06.028;
RA Mishra D., Miyamoto T., Rezenom Y.H., Broussard A., Yavuz A., Slone J.,
RA Russell D.H., Amrein H.;
RT "The molecular basis of sugar sensing in Drosophila larvae.";
RL Curr. Biol. 23:1466-1471(2013).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23418550; DOI=10.1371/journal.pone.0056304;
RA Miyamoto T., Chen Y., Slone J., Amrein H.;
RT "Identification of a Drosophila glucose receptor using Ca2+ imaging of
RT single chemosensory neurons.";
RL PLoS ONE 8:E56304-E56304(2013).
CC -!- FUNCTION: One of the few identified sugar gustatory receptors
CC identified so far with glucose being its primary ligand and which
CC mediates acceptance behavior. {ECO:0000269|PubMed:17715294,
CC ECO:0000269|PubMed:23418550, ECO:0000269|PubMed:23850280}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in sweet sensing neurons of classical
CC chemosensory sensilla, but also in two supersensitive neurons of
CC atypical taste sensilla. {ECO:0000269|PubMed:17715294,
CC ECO:0000269|PubMed:23418550}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr5a subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAF47421.2; -; Genomic_DNA.
DR RefSeq; NP_001261226.1; NM_001274297.1.
DR RefSeq; NP_523877.1; NM_079153.4.
DR AlphaFoldDB; Q9W0M2; -.
DR BioGRID; 63653; 1.
DR IntAct; Q9W0M2; 1.
DR STRING; 7227.FBpp0072482; -.
DR GlyGen; Q9W0M2; 1 site.
DR PaxDb; Q9W0M2; -.
DR PRIDE; Q9W0M2; -.
DR EnsemblMetazoa; FBtr0072585; FBpp0072482; FBgn0035167.
DR EnsemblMetazoa; FBtr0331401; FBpp0303818; FBgn0035167.
DR GeneID; 38098; -.
DR KEGG; dme:Dmel_CG13888; -.
DR CTD; 38098; -.
DR FlyBase; FBgn0035167; Gr61a.
DR VEuPathDB; VectorBase:FBgn0035167; -.
DR eggNOG; ENOG502QTKP; Eukaryota.
DR GeneTree; ENSGT00530000064347; -.
DR HOGENOM; CLU_043581_1_0_1; -.
DR InParanoid; Q9W0M2; -.
DR OMA; TFLWNFM; -.
DR OrthoDB; 622663at2759; -.
DR PhylomeDB; Q9W0M2; -.
DR BioGRID-ORCS; 38098; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38098; -.
DR PRO; PR:Q9W0M2; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035167; Expressed in eye disc (Drosophila) and 3 other tissues.
DR ExpressionAtlas; Q9W0M2; baseline and differential.
DR Genevisible; Q9W0M2; DM.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033041; F:sweet taste receptor activity; IMP:FlyBase.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0007606; P:sensory perception of chemical stimulus; IBA:GO_Central.
DR GO; GO:0050916; P:sensory perception of sweet taste; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR009318; Gustatory_rcpt.
DR PANTHER; PTHR21421; PTHR21421; 1.
DR Pfam; PF06151; Trehalose_recp; 1.
DR PIRSF; PIRSF038981; GRP; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..436
FT /note="Gustatory receptor for sugar taste 61a"
FT /id="PRO_0000216526"
FT TOPO_DOM 1..78
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 79..99
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..111
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 112..132
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 165..185
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..214
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 215..235
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..237
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 238..258
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 259..304
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 305..325
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..334
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 335..355
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 356..436
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 201
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 436 AA; 49920 MW; B31F6C3A98C7FA26 CRC64;
MSRTSDDIRK HLKVRRQKQR AILAMRWRCA QGGLEFEQLD TFYGAIRPYL CVAQFFGIMP
LSNIRSRDPQ DVKFKVRSIG LAVTGLFLLL GGMKTLVGAN ILFTEGLNAK NIVGLVFLIV
GMVNWLNFVG FARSWSHIML PWSSVDILML FPPYKRGKRS LRSKVNVLAL SVVVLAVGDH
MLYYASGYCS YSMHILQCHT NHSRITFGLY LEKEFSDIMF IMPFNIFSMC YGFWLNGAFT
FLWNFMDIFI VMTSIGLAQR FQQFAARVGA LEGRHVPEAL WYDIRRDHIR LCELASLVEA
SMSNIVFVSC ANNVYVICNQ ALAIFTKLRH PINYVYFWYS LIFLLARTSL VFMTASKIHD
ASLLPLRSLY LVPSDGWTQE VQRFADQLTS EFVGLSGYRL FCLTRKSLFG MLATLVTYEL
MLLQIDAKSH KGLRCA
