GR63A_DROME
ID GR63A_DROME Reviewed; 512 AA.
AC Q9VZL7; A1C3K8; B7Z0B4;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Gustatory and odorant receptor 63a;
GN Name=Gr63a; ORFNames=CG14979;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17167414; DOI=10.1038/nature05466;
RA Jones W.D., Cayirlioglu P., Grunwald Kadow I., Vosshall L.B.;
RT "Two chemosensory receptors together mediate carbon dioxide detection in
RT Drosophila.";
RL Nature 445:86-90(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=11257221; DOI=10.1016/s0092-8674(01)00263-x;
RA Scott K., Brady R. Jr., Cravchik A., Morozov P., Rzhetsky A., Zuker C.,
RA Axel R.;
RT "A chemosensory gene family encoding candidate gustatory and olfactory
RT receptors in Drosophila.";
RL Cell 104:661-673(2001).
RN [5]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [6]
RP FUNCTION.
RX PubMed=17493811; DOI=10.1016/j.cub.2007.04.046;
RA Suh G.S., Ben-Tabou de Leon S., Tanimoto H., Fiala A., Benzer S.,
RA Anderson D.J.;
RT "Light activation of an innate olfactory avoidance response in
RT Drosophila.";
RL Curr. Biol. 17:905-908(2007).
RN [7]
RP FUNCTION, AND SUBUNIT.
RX PubMed=17360684; DOI=10.1073/pnas.0700079104;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "The molecular basis of CO2 reception in Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:3574-3578(2007).
RN [8]
RP FUNCTION.
RX PubMed=19710651; DOI=10.1038/nature08295;
RA Turner S.L., Ray A.;
RT "Modification of CO2 avoidance behaviour in Drosophila by inhibitory
RT odorants.";
RL Nature 461:277-281(2009).
RN [9]
RP INDUCTION.
RX PubMed=23105004; DOI=10.1101/gad.201665.112;
RA Sim C.K., Perry S., Tharadra S.K., Lipsick J.S., Ray A.;
RT "Epigenetic regulation of olfactory receptor gene expression by the Myb-
RT MuvB/dREAM complex.";
RL Genes Dev. 26:2483-2498(2012).
RN [10]
RP FUNCTION.
RX PubMed=23185459; DOI=10.1371/journal.pone.0049848;
RA Badsha F., Kain P., Prabhakar S., Sundaram S., Padinjat R., Rodrigues V.,
RA Hasan G.;
RT "Mutants in Drosophila TRPC channels reduce olfactory sensitivity to carbon
RT dioxide.";
RL PLoS ONE 7:E49848-E49848(2012).
CC -!- FUNCTION: Gustatory and odorant receptor which mediates acceptance or
CC avoidance behavior, depending on its substrates. Gr21a and Gr63a
CC together are sufficient for carbon dioxide detection and avoidance
CC behavior. It is possible that the CO(2) receptors Gr63a and Gr21a
CC activate the TRPC channels through Galpha49B and Plc21C. This innate
CC olfactory avoidance behavior can be inhibited by inhibitory
CC interactions of the odors such as 1-hexanol and 2,3-butanedione with
CC Gr21a and Gr63a. {ECO:0000269|PubMed:17167414,
CC ECO:0000269|PubMed:17360684, ECO:0000269|PubMed:17493811,
CC ECO:0000269|PubMed:19710651, ECO:0000269|PubMed:23185459}.
CC -!- SUBUNIT: Gr21a and Gr63a probably form a heterodimer that responds to
CC CO(2). {ECO:0000269|PubMed:17360684}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the medial aspect of the third
CC antennal segment. Carbon dioxide-responsive neurons coexpress Gr21a and
CC Gr63a in a pair of chemosensory receptors at both larval and adult life
CC stages. {ECO:0000269|PubMed:11257221, ECO:0000269|PubMed:17167414}.
CC -!- INDUCTION: The Myb-MuvB complex mediates neuron-specific expression of
CC the carbon dioxide receptor genes Gr63a and Gr21a. Conversely, Mip120
CC and E2F2, are required for repression of Gr63a in inappropriate
CC neurons. {ECO:0000269|PubMed:23105004}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr21a subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACL83239.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; DQ989012; ABK97613.1; -; Genomic_DNA.
DR EMBL; AE014296; ACL83239.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_001137883.1; NM_001144411.1.
DR AlphaFoldDB; Q9VZL7; -.
DR SMR; Q9VZL7; -.
DR STRING; 7227.FBpp0113004; -.
DR TCDB; 1.A.69.2.1; the heteromeric odorant receptor channel (horc) family.
DR GlyGen; Q9VZL7; 2 sites.
DR PaxDb; Q9VZL7; -.
DR PRIDE; Q9VZL7; -.
DR EnsemblMetazoa; FBtr0114512; FBpp0113004; FBgn0035468.
DR GeneID; 38453; -.
DR KEGG; dme:Dmel_CG14979; -.
DR CTD; 38453; -.
DR FlyBase; FBgn0035468; Gr63a.
DR VEuPathDB; VectorBase:FBgn0035468; -.
DR eggNOG; ENOG502SPS2; Eukaryota.
DR GeneTree; ENSGT00530000064835; -.
DR HOGENOM; CLU_049090_0_0_1; -.
DR InParanoid; Q9VZL7; -.
DR OrthoDB; 809607at2759; -.
DR PhylomeDB; Q9VZL7; -.
DR BioGRID-ORCS; 38453; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38453; -.
DR PRO; PR:Q9VZL7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035468; Expressed in olfactory receptor neuron (Drosophila) and 2 other tissues.
DR Genevisible; Q9VZL7; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004984; F:olfactory receptor activity; IBA:GO_Central.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0007610; P:behavior; IEA:UniProtKB-KW.
DR GO; GO:0003031; P:detection of carbon dioxide; IGI:FlyBase.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:FlyBase.
DR GO; GO:0010037; P:response to carbon dioxide; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; NAS:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 1: Evidence at protein level;
KW Behavior; Cell membrane; Glycoprotein; Membrane; Olfaction; Receptor;
KW Reference proteome; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..512
FT /note="Gustatory and odorant receptor 63a"
FT /id="PRO_0000216527"
FT TOPO_DOM 1..129
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 130..150
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..166
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 167..187
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..222
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 223..243
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..265
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 266..285
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..324
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 325..345
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 346..350
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 351..371
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..436
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 437..457
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 458..512
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 261
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 468
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 512 AA; 57465 MW; B98BD94D6D4BFFF6 CRC64;
MRPSGEKVVK GHGQGNSGHS LSGMANYYRR KKGDAVFLNA KPLNSANAQA YLYGVRKYSI
GLAERLDADY EAPPLDRKKS SDSTASNNPE FKPSVFYRNI DPINWFLRII GVLPIVRHGP
ARAKFEMNSA SFIYSVVFFV LLACYVGYVA NNRIHIVRSL SGPFEEAVIA YLFLVNILPI
MIIPILWYEA RKIAKLFNDW DDFEVLYYQI SGHSLPLKLR QKAVYIAIVL PILSVLSVVI
THVTMSDLNI NQVVPYCILD NLTAMLGAWW FLICEAMSIT AHLLAERFQK ALKHIGPAAM
VADYRVLWLR LSKLTRDTGN ALCYTFVFMS LYLFFIITLS IYGLMSQLSE GFGIKDIGLT
ITALWNIGLL FYICDEAHYA SVNVRTNFQK KLLMVELNWM NSDAQTEINM FLRATEMNPS
TINCGGFFDV NRTLFKGLLT TMVTYLVVLL QFQISIPTDK GDSEGANNIT VVDFVMDSLD
NDMSLMGAST LSTTTVGTTL PPPIMKLKGR KG
