GR64A_DROME
ID GR64A_DROME Reviewed; 456 AA.
AC P83293; Q9VZJ7;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2002, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Gustatory receptor for sugar taste 64a;
GN Name=Gr64a; ORFNames=CG32261;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP PREDICTION OF FUNCTION.
RX PubMed=14608037; DOI=10.1073/pnas.2335847100;
RA Robertson H.M., Warr C.G., Carlson J.R.;
RT "Molecular evolution of the insect chemoreceptor gene superfamily in
RT Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14537-14542(2003).
RN [5]
RP FUNCTION.
RX PubMed=17988633; DOI=10.1016/j.neuron.2007.10.024;
RA Dahanukar A., Lei Y.T., Kwon J.Y., Carlson J.R.;
RT "Two Gr genes underlie sugar reception in Drosophila.";
RL Neuron 56:503-516(2007).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17715294; DOI=10.1073/pnas.0702421104;
RA Jiao Y., Moon S.J., Montell C.;
RT "A Drosophila gustatory receptor required for the responses to sucrose,
RT glucose, and maltose identified by mRNA tagging.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:14110-14115(2007).
RN [7]
RP FUNCTION.
RX PubMed=19026541; DOI=10.1016/j.cub.2008.10.009;
RA Jiao Y., Moon S.J., Wang X., Ren Q., Montell C.;
RT "Gr64f is required in combination with other gustatory receptors for sugar
RT detection in Drosophila.";
RL Curr. Biol. 18:1797-1801(2008).
RN [8]
RP FUNCTION.
RX PubMed=21709242; DOI=10.1073/pnas.1017096108;
RA Dus M., Min S., Keene A.C., Lee G.Y., Suh G.S.;
RT "Taste-independent detection of the caloric content of sugar in
RT Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:11644-11649(2011).
RN [9]
RP FUNCTION, AND INDUCTION.
RX PubMed=22794108; DOI=10.3109/01677063.2012.694931;
RA Nishimura A., Ishida Y., Takahashi A., Okamoto H., Sakabe M., Itoh M.,
RA Takano-Shimizu T., Ozaki M.;
RT "Starvation-induced elevation of taste responsiveness and expression of a
RT sugar taste receptor gene in Drosophila melanogaster.";
RL J. Neurogenet. 26:206-215(2012).
RN [10]
RP FUNCTION.
RX PubMed=23850280; DOI=10.1016/j.cub.2013.06.028;
RA Mishra D., Miyamoto T., Rezenom Y.H., Broussard A., Yavuz A., Slone J.,
RA Russell D.H., Amrein H.;
RT "The molecular basis of sugar sensing in Drosophila larvae.";
RL Curr. Biol. 23:1466-1471(2013).
CC -!- FUNCTION: One of the few identified sugar gustatory receptors
CC identified so far and which promotes the starvation-induced increase of
CC feeding motivation. Required in combination with Gr64f to detect
CC sucrose, maltose, and glucose. {ECO:0000269|PubMed:17715294,
CC ECO:0000269|PubMed:17988633, ECO:0000269|PubMed:19026541,
CC ECO:0000269|PubMed:21709242, ECO:0000269|PubMed:22794108,
CC ECO:0000269|PubMed:23850280}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in Gr5a-expressing sugar-sensing cells.
CC {ECO:0000269|PubMed:17715294}.
CC -!- INDUCTION: Expression is increased by starvation.
CC {ECO:0000269|PubMed:22794108}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr5a subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAN11576.1; -; Genomic_DNA.
DR RefSeq; NP_728920.1; NM_168048.2.
DR AlphaFoldDB; P83293; -.
DR SMR; P83293; -.
DR STRING; 7227.FBpp0073050; -.
DR GlyGen; P83293; 1 site.
DR PaxDb; P83293; -.
DR EnsemblMetazoa; FBtr0073194; FBpp0073050; FBgn0045479.
DR GeneID; 117481; -.
DR KEGG; dme:Dmel_CG32261; -.
DR CTD; 117481; -.
DR FlyBase; FBgn0045479; Gr64a.
DR VEuPathDB; VectorBase:FBgn0045479; -.
DR eggNOG; ENOG502QTKP; Eukaryota.
DR GeneTree; ENSGT00530000064347; -.
DR HOGENOM; CLU_043581_1_0_1; -.
DR InParanoid; P83293; -.
DR OMA; FVCCQLL; -.
DR OrthoDB; 622663at2759; -.
DR PhylomeDB; P83293; -.
DR BioGRID-ORCS; 117481; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 117481; -.
DR PRO; PR:P83293; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0045479; Expressed in wing disc and 1 other tissue.
DR ExpressionAtlas; P83293; baseline and differential.
DR Genevisible; P83293; DM.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033041; F:sweet taste receptor activity; IMP:FlyBase.
DR GO; GO:0008527; F:taste receptor activity; ISS:FlyBase.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; IGI:FlyBase.
DR GO; GO:0051594; P:detection of glucose; IMP:FlyBase.
DR GO; GO:0034289; P:detection of maltose stimulus; IMP:FlyBase.
DR GO; GO:0009731; P:detection of sucrose stimulus; IMP:FlyBase.
DR GO; GO:0007637; P:proboscis extension reflex; IMP:FlyBase.
DR GO; GO:0007606; P:sensory perception of chemical stimulus; IBA:GO_Central.
DR GO; GO:0050916; P:sensory perception of sweet taste; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; ISS:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR009318; Gustatory_rcpt.
DR PANTHER; PTHR21421; PTHR21421; 1.
DR Pfam; PF06151; Trehalose_recp; 1.
DR PIRSF; PIRSF038981; GRP; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..456
FT /note="Gustatory receptor for sugar taste 64a"
FT /id="PRO_0000216528"
FT TOPO_DOM 1..61
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 62..82
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..95
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 96..116
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 117..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 129..149
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..185
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 186..206
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 239..259
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..321
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 322..342
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..351
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 352..372
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373..456
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 177
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 456 AA; 52823 MW; 5466974C73495D6C CRC64;
MKGPNLNFRK TPSKDNGVKQ VESLARPETP PPKFVEDSNL EFNVLASEKL PNYTNLDLFH
RAVFPFMFLA QCVAIMPLVG IRESNPRRVR FAYKSIPMFV TLIFMIATSI LFLSMFTHLL
KIGITAKNFV GLVFFGCVLS AYVVFIRLAK KWPAVVRIWT RTEIPFTKPP YEIPKRNLSR
RVQLAALAII GLSLGEHALY QVSAILSYTR RIQMCANITT VPSFNNYMQT NYDYVFQLLP
YSPIIAVLIL LINGACTFVW NYMDLFIMMI SKGLSYRFEQ ITTRIRKLEH EEVCESVFIQ
IREHYVKMCE LLEFVDSAMS SLILLSCVNN LYFVCYQLLN VFNKLRWPIN YIYFWYSLLY
LIGRTAFVFL TAADINEESK RGLGVLRRVS SRSWCVEVER LIFQMTTQTV ALSGKKFYFL
TRRLLFGMAG TIVTYELVLL QFDEPNRRKG LQPLCA
