GR64D_DROME
ID GR64D_DROME Reviewed; 417 AA.
AC Q9VZJ6;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 07-SEP-2016, sequence version 3.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Gustatory receptor for sugar taste 64d;
GN Name=Gr64d; ORFNames=CG14987;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP PREDICTION OF FUNCTION.
RX PubMed=14608037; DOI=10.1073/pnas.2335847100;
RA Robertson H.M., Warr C.G., Carlson J.R.;
RT "Molecular evolution of the insect chemoreceptor gene superfamily in
RT Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14537-14542(2003).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17715294; DOI=10.1073/pnas.0702421104;
RA Jiao Y., Moon S.J., Montell C.;
RT "A Drosophila gustatory receptor required for the responses to sucrose,
RT glucose, and maltose identified by mRNA tagging.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:14110-14115(2007).
RN [6]
RP FUNCTION.
RX PubMed=23850280; DOI=10.1016/j.cub.2013.06.028;
RA Mishra D., Miyamoto T., Rezenom Y.H., Broussard A., Yavuz A., Slone J.,
RA Russell D.H., Amrein H.;
RT "The molecular basis of sugar sensing in Drosophila larvae.";
RL Curr. Biol. 23:1466-1471(2013).
CC -!- FUNCTION: One of the few identified sugar gustatory receptors
CC identified so far and which promotes the starvation-induced increase of
CC feeding motivation. {ECO:0000269|PubMed:17715294,
CC ECO:0000269|PubMed:23850280}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=C {ECO:0000312|FlyBase:FBgn0035486};
CC IsoId=Q9VZJ6-1; Sequence=Displayed;
CC Name=A {ECO:0000312|FlyBase:FBgn0035486};
CC IsoId=Q9VZJ6-2; Sequence=VSP_012751;
CC -!- TISSUE SPECIFICITY: Expressed in Gr5a-expressing sugar-sensing cells.
CC {ECO:0000269|PubMed:17715294}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr5a subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAF47825.3; -; Genomic_DNA.
DR EMBL; AE014296; AAS64968.2; -; Genomic_DNA.
DR RefSeq; NP_001027105.2; NM_001031934.1. [Q9VZJ6-1]
DR RefSeq; NP_728922.2; NM_168050.3. [Q9VZJ6-2]
DR AlphaFoldDB; Q9VZJ6; -.
DR SMR; Q9VZJ6; -.
DR STRING; 7227.FBpp0099615; -.
DR GlyGen; Q9VZJ6; 1 site.
DR PaxDb; Q9VZJ6; -.
DR EnsemblMetazoa; FBtr0073197; FBpp0099614; FBgn0035486. [Q9VZJ6-2]
DR EnsemblMetazoa; FBtr0344475; FBpp0310846; FBgn0035486. [Q9VZJ6-1]
DR GeneID; 3772638; -.
DR KEGG; dme:Dmel_CG14987; -.
DR CTD; 3772638; -.
DR FlyBase; FBgn0035486; Gr64d.
DR VEuPathDB; VectorBase:FBgn0035486; -.
DR eggNOG; ENOG502T85S; Eukaryota.
DR GeneTree; ENSGT00530000064347; -.
DR InParanoid; Q9VZJ6; -.
DR OMA; MGYFFVT; -.
DR BioGRID-ORCS; 3772638; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 3772638; -.
DR PRO; PR:Q9VZJ6; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033041; F:sweet taste receptor activity; IMP:FlyBase.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0007606; P:sensory perception of chemical stimulus; IBA:GO_Central.
DR GO; GO:0050916; P:sensory perception of sweet taste; IMP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR009318; Gustatory_rcpt.
DR PANTHER; PTHR21421; PTHR21421; 1.
DR Pfam; PF06151; Trehalose_recp; 1.
DR PIRSF; PIRSF038981; GRP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..417
FT /note="Gustatory receptor for sugar taste 64d"
FT /id="PRO_0000216531"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 12..32
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..45
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 46..66
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..77
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 78..98
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..137
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 138..158
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..193
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 194..214
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..302
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 303..323
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..374
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 375..395
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 396..417
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..64
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_012751"
SQ SEQUENCE 417 AA; 48869 MW; 888A8AD4D6A4C484 CRC64;
MERSVQENTL HYTIGHVLII ARIFGVLPLA GINPNGKPEN VRFRWFSPYI LFFVVAFTFV
IADFMLSTKI VLNDGLQLYT MGSLSFSVIC IFCFGSFIKL SRRWPHIIRE TALCERIFLK
PCYANQEGLN FTRFLRRWAL ILLVAALCEH LTYVGSAAWS NYVQIRDCNL KVGFVENYFL
RERQELFSVF EYRAWMVFFI EWNTMAMTFV WNFGDIFLFL MCRGLKIRFQ QLHWRIRQNL
GKPMAKEFWQ EIRSDFLDLD SLLKLYDKEL SGLILVCCAH NMYFICVQVY HSFQVKGAFM
DELYFWFCLL YVISRLMNMM LAASSIPQEI KDISNTLYEV RSSPWCDELG RLSEMLRNET
FALSGMGYFY VTRRLIFAMA GALMGYELVL FRQMQGAVVQ KSICSRGPGS SMSIFFS
