GR64E_DROME
ID GR64E_DROME Reviewed; 451 AA.
AC P83296; Q8IRC5; Q9VZJ5;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 25-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Gustatory receptor for sugar taste 64e;
GN Name=Gr64e; ORFNames=CG14988;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [5]
RP PREDICTION OF FUNCTION.
RX PubMed=14608037; DOI=10.1073/pnas.2335847100;
RA Robertson H.M., Warr C.G., Carlson J.R.;
RT "Molecular evolution of the insect chemoreceptor gene superfamily in
RT Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14537-14542(2003).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17715294; DOI=10.1073/pnas.0702421104;
RA Jiao Y., Moon S.J., Montell C.;
RT "A Drosophila gustatory receptor required for the responses to sucrose,
RT glucose, and maltose identified by mRNA tagging.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:14110-14115(2007).
RN [7]
RP FUNCTION.
RX PubMed=22057190; DOI=10.1038/nn.2944;
RA Wisotsky Z., Medina A., Freeman E., Dahanukar A.;
RT "Evolutionary differences in food preference rely on Gr64e, a receptor for
RT glycerol.";
RL Nat. Neurosci. 14:1534-1541(2011).
RN [8]
RP FUNCTION.
RX PubMed=23850280; DOI=10.1016/j.cub.2013.06.028;
RA Mishra D., Miyamoto T., Rezenom Y.H., Broussard A., Yavuz A., Slone J.,
RA Russell D.H., Amrein H.;
RT "The molecular basis of sugar sensing in Drosophila larvae.";
RL Curr. Biol. 23:1466-1471(2013).
CC -!- FUNCTION: One of the few identified sugar gustatory receptors
CC identified so far and which promotes the starvation-induced increase of
CC feeding motivation. confers responsiveness to glycerol and is required
CC for feeding preference for beer and other sources that have fermenting
CC yeast. {ECO:0000269|PubMed:17715294, ECO:0000269|PubMed:22057190,
CC ECO:0000269|PubMed:23850280}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in Gr5a-expressing sugar-sensing cells.
CC {ECO:0000269|PubMed:17715294}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr5a subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAN11578.2; -; Genomic_DNA.
DR EMBL; BT003632; AAO39636.1; -; mRNA.
DR RefSeq; NP_728923.2; NM_168051.3.
DR AlphaFoldDB; P83296; -.
DR SMR; P83296; -.
DR STRING; 7227.FBpp0099916; -.
DR PaxDb; P83296; -.
DR DNASU; 3771916; -.
DR EnsemblMetazoa; FBtr0073199; FBpp0099915; FBgn0045476.
DR GeneID; 3771916; -.
DR KEGG; dme:Dmel_CG32258; -.
DR CTD; 3771916; -.
DR FlyBase; FBgn0045476; Gr64e.
DR VEuPathDB; VectorBase:FBgn0045476; -.
DR eggNOG; ENOG502QTKP; Eukaryota.
DR GeneTree; ENSGT00530000064347; -.
DR HOGENOM; CLU_043581_0_0_1; -.
DR InParanoid; P83296; -.
DR OMA; SHTAYFW; -.
DR PhylomeDB; P83296; -.
DR BioGRID-ORCS; 3771916; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 3771916; -.
DR PRO; PR:P83296; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0045476; Expressed in testis and 1 other tissue.
DR Genevisible; P83296; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033041; F:sweet taste receptor activity; IMP:FlyBase.
DR GO; GO:0007606; P:sensory perception of chemical stimulus; IBA:GO_Central.
DR GO; GO:0050916; P:sensory perception of sweet taste; IMP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR009318; Gustatory_rcpt.
DR PANTHER; PTHR21421; PTHR21421; 1.
DR Pfam; PF06151; Trehalose_recp; 1.
DR PIRSF; PIRSF038981; GRP; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..451
FT /note="Gustatory receptor for sugar taste 64e"
FT /id="PRO_0000216532"
FT TOPO_DOM 1..100
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 101..121
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 122..130
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 131..151
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..183
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 184..204
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..223
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 224..244
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 245..250
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 251..271
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..344
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 345..365
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 366..420
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 421..441
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 442..446
FT /note="Extracellular"
FT /evidence="ECO:0000250"
SQ SEQUENCE 451 AA; 52530 MW; 0C2471D9EA0B99BE CRC64;
MARTTGDPAK RRRCMSRIKF WRRSRVGSEV VEKDTKRFKL SLIKAWLLRI RQEDYKYSGS
FQEAIKPVLI IAQIFALMPV RKVSSKFAED LTFTWFSVRS YYALVTILFF GVSSGYMVAF
VTSVSFNFDS VETLVFYLSI FLISLSFFQL ARKWPEIAQS WQLVEAKLPP LKLPKERRSL
AQHINMITIV ATTCSLVEHI MSMLSMGYYV NSCPRWPDRP IDSFLYLSFS SVFYFVDYTR
FLGIVGKVVN VLSTFAWNFN DIFVMAVSVA LAARFRQLND YMMREARLPT TVDYWMQCRI
NFRNLCKLCE EVDDAISTIT LLCFSNNLYF ICGKILKSMQ AKPSIWHALY FWFSLVYLLG
RTLILSLYSS SINDESKRPL VIFRLVPREY WCDELKRFSE EVQMDNVALT GMKFFRLTRG
VVISVAGTIV TYELILLQFN GEEKVPGCFE N
