GR66A_DROME
ID GR66A_DROME Reviewed; 527 AA.
AC Q9VSH2;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 3.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Gustatory receptor for bitter taste 66a;
GN Name=Gr66a; ORFNames=CG7189;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=15202999; DOI=10.1016/j.cub.2004.05.019;
RA Thorne N., Chromey C., Bray S., Amrein H.;
RT "Taste perception and coding in Drosophila.";
RL Curr. Biol. 14:1065-1079(2004).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=16979558; DOI=10.1016/j.cub.2006.07.024;
RA Moon S.J., Kottgen M., Jiao Y., Xu H., Montell C.;
RT "A taste receptor required for the caffeine response in vivo.";
RL Curr. Biol. 16:1812-1817(2006).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16423701; DOI=10.1016/j.neuron.2005.11.037;
RA Marella S., Fischler W., Kong P., Asgarian S., Rueckert E., Scott K.;
RT "Imaging taste responses in the fly brain reveals a functional map of taste
RT category and behavior.";
RL Neuron 49:285-295(2006).
RN [7]
RP FUNCTION.
RX PubMed=19618260; DOI=10.1007/s10519-009-9286-8;
RA Lacaille F., Everaerts C., Ferveur J.F.;
RT "Feminization and alteration of Drosophila taste neurons induce reciprocal
RT effects on male avoidance behavior.";
RL Behav. Genet. 39:554-563(2009).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19246397; DOI=10.1073/pnas.0811744106;
RA Lee Y., Moon S.J., Montell C.;
RT "Multiple gustatory receptors required for the caffeine response in
RT Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:4495-4500(2009).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20797533; DOI=10.1016/j.neuron.2010.07.006;
RA Lee Y., Kim S.H., Montell C.;
RT "Avoiding DEET through insect gustatory receptors.";
RL Neuron 67:555-561(2010).
RN [10]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
RN [11]
RP FUNCTION.
RX PubMed=22798487; DOI=10.1534/genetics.112.142455;
RA Joseph R.M., Heberlein U.;
RT "Tissue-specific activation of a single gustatory receptor produces
RT opposing behavioral responses in Drosophila.";
RL Genetics 192:521-532(2012).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22279227; DOI=10.1523/jneurosci.4630-11.2012;
RA Lee Y., Kang M.J., Shim J., Cheong C.U., Moon S.J., Montell C.;
RT "Gustatory receptors required for avoiding the insecticide L-canavanine.";
RL J. Neurosci. 32:1429-1435(2012).
CC -!- FUNCTION: Gustatory receptor required for response to the bitter in
CC taste neurons. Gr66a cells respond to bitter compounds such as
CC caffeine, theophylline, threonine or valine. Flies avoid bitter
CC substances, suggesting that Gr66a neuron activity is sufficient to
CC mediate avoidance behavior. Required for sensing and avoiding N,N-
CC Diethyl-meta-toluamide (DEET), the most widely used insect repellent
CC worldwide, as well as to L-canavanine, a plant-derived insecticide.
CC Gr66a neurons are also involved in the sex-specific perception of
CC molecules inducing male avoidance behavior, probably through sensing 7-
CC tricosene (7-T), a male cuticular pheromone and leading to inhibition
CC of male-male courtship. Finally, also plays a role in oviposition
CC behavior, in which females evaluate their environment and choose to lay
CC eggs on substrates they may find aversive in other contexts.
CC {ECO:0000269|PubMed:15202999, ECO:0000269|PubMed:16423701,
CC ECO:0000269|PubMed:16979558, ECO:0000269|PubMed:19246397,
CC ECO:0000269|PubMed:19618260, ECO:0000269|PubMed:20797533,
CC ECO:0000269|PubMed:22279227, ECO:0000269|PubMed:22798487}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Taste hairs in labial palps, labral and cibarial
CC sense organs and forelegs. In larvae, is expressed in neurons of the
CC terminal external chemosensory organ, as well as in the dorsal,
CC ventral, and posterior pharyngeal sense organs.
CC {ECO:0000269|PubMed:11516643, ECO:0000269|PubMed:15202999,
CC ECO:0000269|PubMed:16423701, ECO:0000269|PubMed:16979558,
CC ECO:0000269|PubMed:22031876}.
CC -!- DISRUPTION PHENOTYPE: Disrupts fly aversion to caffeine, N,N-Diethyl-
CC meta-toluamide (DEET), and L-canavanine. {ECO:0000269|PubMed:16979558,
CC ECO:0000269|PubMed:19246397, ECO:0000269|PubMed:20797533,
CC ECO:0000269|PubMed:22279227}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr66a subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAF50447.3; -; Genomic_DNA.
DR RefSeq; NP_523971.3; NM_079247.3.
DR AlphaFoldDB; Q9VSH2; -.
DR STRING; 7227.FBpp0289508; -.
DR GlyGen; Q9VSH2; 2 sites.
DR PaxDb; Q9VSH2; -.
DR EnsemblMetazoa; FBtr0300280; FBpp0289508; FBgn0035870.
DR GeneID; 38935; -.
DR KEGG; dme:Dmel_CG7189; -.
DR CTD; 38935; -.
DR FlyBase; FBgn0035870; Gr66a.
DR VEuPathDB; VectorBase:FBgn0035870; -.
DR eggNOG; ENOG502S2QD; Eukaryota.
DR GeneTree; ENSGT00940000166130; -.
DR HOGENOM; CLU_555838_0_0_1; -.
DR InParanoid; Q9VSH2; -.
DR OMA; SWKTSQA; -.
DR OrthoDB; 550351at2759; -.
DR PhylomeDB; Q9VSH2; -.
DR SignaLink; Q9VSH2; -.
DR BioGRID-ORCS; 38935; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38935; -.
DR PRO; PR:Q9VSH2; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035870; Expressed in gustatory receptor neuron (Drosophila) and 4 other tissues.
DR ExpressionAtlas; Q9VSH2; baseline and differential.
DR Genevisible; Q9VSH2; DM.
DR GO; GO:0030424; C:axon; IDA:FlyBase.
DR GO; GO:0030425; C:dendrite; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0007635; P:chemosensory behavior; IMP:FlyBase.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0031000; P:response to caffeine; IMP:FlyBase.
DR GO; GO:0017085; P:response to insecticide; IMP:FlyBase.
DR GO; GO:1901354; P:response to L-canavanine; IMP:FlyBase.
DR GO; GO:0050913; P:sensory perception of bitter taste; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; NAS:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 2.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..527
FT /note="Gustatory receptor for bitter taste 66a"
FT /id="PRO_0000216535"
FT TOPO_DOM 1..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 47..67
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..80
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 81..101
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..144
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 145..165
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166..174
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 175..195
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..345
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 346..366
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 367..382
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 383..403
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 404..460
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 461..481
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 482..527
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 496
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 510
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 527 AA; 59081 MW; E3C0F6335C89B232 CRC64;
MAQAEDAVQP LLQQFQQLFF ISKIAGILPQ DLEKFRSRNL LEKSRNGMIY MLSTLILYVV
LYNILIYSFG EEDRSLKASQ STLTFVIGLF LTYIGLIMMV SDQLTALRNQ GRIGELYERI
RLVDERLYKE GCVMDNSTIG RRIRIMLIMT VIFELSILVS TYVKLVDYSQ WMSLLWIVSA
IPTFINTLDK IWFAVSLYAL KERFEAINAT LEELVDTHEK HKLWLRGNQE VPPPLDSSQP
PQYDSNLEYL YKELGGMDIG SIGKSSVSGS GKNKVAPVAH SMNSFGEAID AASRKPPPPP
LATNMVHESE LGNAAKVEEK LNNLCQVHDE ICEIGKALNE LWSYPILSLM AYGFLIFTAQ
LYFLYCATQY QSIPSLFRSA KNPFITVIVL SYTSGKCVYL IYLSWKTSQA SKRTGISLHK
CGVVADDNLL YEIVNHLSLK LLNHSVDFSA CGFFTLDMET LYGVSGGITS YLIILIQFNL
AAQQAKEAIQ TFNSLNDTAG LVGAATDMDN ISSTLRDFVT TTMTPAV
