GR68A_DROME
ID GR68A_DROME Reviewed; 389 AA.
AC Q9VTN0;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Gustatory receptor 68a;
GN Name=Gr68a; Synonyms=GR68D.1; ORFNames=CG7303;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12971900; DOI=10.1016/s0896-6273(03)00542-7;
RA Bray S., Amrein H.;
RT "A putative Drosophila pheromone receptor expressed in male-specific taste
RT neurons is required for efficient courtship.";
RL Neuron 39:1019-1029(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26083710; DOI=10.7554/elife.06914;
RA Shankar S., Chua J.Y., Tan K.J., Calvert M.E., Weng R., Ng W.C., Mori K.,
RA Yew J.Y.;
RT "The neuropeptide tachykinin is essential for pheromone detection in a
RT gustatory neural circuit.";
RL Elife 4:E06914-E06914(2015).
CC -!- FUNCTION: Dsx-dependent essential component of pheromone-driven
CC courtship behavior (PubMed:12971900). Recognizes a female pheromone
CC involved in the second step (tapping step) of the courtship display
CC which is essential for efficient execution of the entire courtship
CC sequence and timely mating (PubMed:12971900). Required for detection of
CC the male sex pheromone CH503 which is transferred from males to females
CC during mating and inhibits courtship behavior by other males
CC (PubMed:26083710). Gr68a-expressing neurons in the male foreleg relay
CC signals to the suboesophageal zone (SEZ) and courtship suppression is
CC mediated by the release of the neuropeptide tachykinin from a cluster
CC of 8-10 neurons in the SEZ (PubMed:26083710).
CC {ECO:0000269|PubMed:12971900, ECO:0000269|PubMed:26083710}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in chemosensory neurons of about 20 male-
CC specific gustatory bristles in the forelegs (PubMed:12971900). No
CC expression is seen in the mechanosensory neurons (PubMed:12971900). In
CC larvae, expressed in the ventral pharyngeal sense organ
CC (PubMed:22031876). {ECO:0000269|PubMed:12971900,
CC ECO:0000269|PubMed:22031876}.
CC -!- DISRUPTION PHENOTYPE: Mutant males display robust courtship behavior in
CC the presence of CH503-perfumed females. {ECO:0000269|PubMed:26083710}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr21a subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAF50017.2; -; Genomic_DNA.
DR RefSeq; NP_524027.2; NM_079303.2.
DR AlphaFoldDB; Q9VTN0; -.
DR IntAct; Q9VTN0; 1.
DR STRING; 7227.FBpp0075849; -.
DR GlyGen; Q9VTN0; 2 sites.
DR PaxDb; Q9VTN0; -.
DR DNASU; 39324; -.
DR EnsemblMetazoa; FBtr0076118; FBpp0075849; FBgn0041231.
DR GeneID; 39324; -.
DR KEGG; dme:Dmel_CG7303; -.
DR CTD; 39324; -.
DR FlyBase; FBgn0041231; Gr68a.
DR VEuPathDB; VectorBase:FBgn0041231; -.
DR eggNOG; ENOG502T08N; Eukaryota.
DR GeneTree; ENSGT00940000166130; -.
DR HOGENOM; CLU_058693_0_0_1; -.
DR InParanoid; Q9VTN0; -.
DR OMA; GRWYGRL; -.
DR OrthoDB; 687132at2759; -.
DR PhylomeDB; Q9VTN0; -.
DR BioGRID-ORCS; 39324; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39324; -.
DR PRO; PR:Q9VTN0; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0041231; Expressed in brain.
DR ExpressionAtlas; Q9VTN0; differential.
DR Genevisible; Q9VTN0; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008527; F:taste receptor activity; ISS:FlyBase.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0016544; P:male courtship behavior, tapping to detect pheromone; IMP:UniProtKB.
DR GO; GO:0019236; P:response to pheromone; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; ISS:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Behavior; Cell membrane; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..389
FT /note="Gustatory receptor 68a"
FT /id="PRO_0000216536"
FT TOPO_DOM 1..42
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 43..63
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..82
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 83..103
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 134..154
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..164
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 165..185
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..236
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 237..257
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..281
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 282..302
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..352
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 353..373
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..389
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 80
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 258
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 389 AA; 43780 MW; 59A55E4895BDEC3B CRC64;
MKIYQDIYPI SKPSQIFAIL PFYSGDVDDG FRFGGLGRWY GRLVALIILI GSLTLGEDVL
FASKEYRLVA SAQGDTEEIN RTIETLLCII SYTMVVLSSV QNASRHFRTL HDIAKIDEYL
LANGFRETYS CRNLTILVTS AAGGVLAVAF YYIHYRSGIG AKRQIILLLI YFLQLLYSTL
LALYLRTLMM NLAQRIGFLN QKLDTFNLQD CGHMENWREL SNLIEVLCKF RYITENINCV
AGVSLLFYFG FSFYTVTNQS YLAFATLTAG SLSSKTEVAD TIGLSCIWVL AETITMIVIC
SACDGLASEV NGTAQILARI YGKSKQFQNL IDKFLTKSIK QDLQFTAYGF FSIDNSTLFK
IFSAVTTYLV ILIQFKQLED SKVEDISQA
