GR93A_DROME
ID GR93A_DROME Reviewed; 419 AA.
AC Q9VD76;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Gustatory receptor for bitter taste 93a;
GN Name=Gr93a; Synonyms=GR93F.1; ORFNames=CG13417;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF55923.2};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=10710312; DOI=10.1126/science.287.5459.1830;
RA Clyne P.J., Warr C.G., Carlson J.R.;
RT "Candidate taste receptors in Drosophila.";
RL Science 287:1830-1834(2000).
RN [4] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19246397; DOI=10.1073/pnas.0811744106;
RA Lee Y., Moon S.J., Montell C.;
RT "Multiple gustatory receptors required for the caffeine response in
RT Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:4495-4500(2009).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
CC -!- FUNCTION: Gustatory receptor required for response to the bitter in
CC taste neurons. Gr93a cells respond to bitter compounds such as
CC caffeine. Flies avoid bitter substances, suggesting that Gr93a neuron
CC activity is sufficient to mediate avoidance behavior.
CC {ECO:0000269|PubMed:19246397}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: In larvae, is expressed in neurons of the dorsal
CC pharyngeal sense organs. {ECO:0000269|PubMed:22031876}.
CC -!- DISRUPTION PHENOTYPE: Disrupts fly aversion to caffeine.
CC {ECO:0000269|PubMed:19246397}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr93a subfamily. {ECO:0000305}.
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DR EMBL; AE014297; AAF55923.2; -; Genomic_DNA.
DR RefSeq; NP_524442.2; NM_079718.3.
DR AlphaFoldDB; Q9VD76; -.
DR STRING; 7227.FBpp0083531; -.
DR PaxDb; Q9VD76; -.
DR EnsemblMetazoa; FBtr0084133; FBpp0083531; FBgn0041229.
DR GeneID; 42568; -.
DR KEGG; dme:Dmel_CG13417; -.
DR CTD; 42568; -.
DR FlyBase; FBgn0041229; Gr93a.
DR VEuPathDB; VectorBase:FBgn0041229; -.
DR eggNOG; ENOG502T83R; Eukaryota.
DR HOGENOM; CLU_614325_0_0_1; -.
DR InParanoid; Q9VD76; -.
DR OMA; MCADYAV; -.
DR OrthoDB; 1174478at2759; -.
DR PhylomeDB; Q9VD76; -.
DR BioGRID-ORCS; 42568; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42568; -.
DR PRO; PR:Q9VD76; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0041229; Expressed in mouthpart and 1 other tissue.
DR ExpressionAtlas; Q9VD76; baseline.
DR Genevisible; Q9VD76; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0031000; P:response to caffeine; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..419
FT /note="Gustatory receptor for bitter taste 93a"
FT /id="PRO_0000216541"
FT TOPO_DOM 1..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 56..76
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..90
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 91..111
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..145
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 146..166
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..184
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 185..205
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..277
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 278..298
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 299..305
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 306..326
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..383
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 384..404
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 405..419
FT /note="Extracellular"
FT /evidence="ECO:0000250"
SQ SEQUENCE 419 AA; 48834 MW; A124B6C5304C1B52 CRC64;
MFSSSSAMTG KRAESWSRLL LLWLYRCARG LLVLSSSLDR DKLQLKATKQ GSRNRFLHIL
WRCIVVMIYA GLWPMLTSAV IGKRLESYAD VLALAQSMSV SILAVISFVI QARGENQFRE
VLNRYLALYQ RICLTTRLRH LFPTKFVVFF LLKLFFTLCG CFHEIIPLFE NSHFDDISQM
VGTGFGIYMW LGTLCVLDAC FLGFLVSGIL YEHMANNIIA MLKRMEPIES QDERYRMTKY
RRMQLLCDFA DELDECAAIY SELYHVTNSF RRILQWQILF YIYLNFINIC LMLYQYILHF
LNDDEVVFVS IVMAFVKLAN LVLLMMCADY TVRQSEVPKK LPLDIVCSDM DERWDKSVET
FLGQLQTQRL EIKVLGFFHL NNEFILLILS AIISYLFILI QFGITGGFEA SEDIKNRFD
