ID   GRA2_NEOCA              Reviewed;         193 AA.
AC   Q25540; O76215;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2003, sequence version 2.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Dense granule protein 2;
DE   AltName: Full=Antigen Nc14.1;
DE   AltName: Full=NcDG2;
GN   Name=DG2;
OS   Neospora caninum (Coccidian parasite).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC   Eucoccidiorida; Eimeriorina; Sarcocystidae; Neospora.
OX   NCBI_TaxID=29176;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Nc-1;
RX   PubMed=9662039; DOI=10.1016/s0166-6851(98)00031-0;
RA   Liddell S., Lally N.C., Jenkins M.C., Dubey J.P.;
RT   "Isolation of the cDNA encoding a dense granule associated antigen (NCDG2)
RT   of Neospora caninum.";
RL   Mol. Biochem. Parasitol. 93:153-158(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 67-193.
RC   STRAIN=Nc-1;
RX   PubMed=8705668; DOI=10.1128/cdli.3.3.275-279.1996;
RA   Lally N.C., Jenkins M.C., Dubey J.P.;
RT   "Evaluation of two Neospora caninum recombinant antigens for use in an
RT   enzyme-linked immunosorbent assay for the diagnosis of bovine
RT   neosporosis.";
RL   Clin. Diagn. Lab. Immunol. 3:275-279(1996).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Gra6 family. {ECO:0000305}.
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DR   EMBL; AF029350; AAC39122.1; -; mRNA.
DR   EMBL; U36387; AAC47097.1; -; mRNA.
DR   AlphaFoldDB; Q25540; -.
DR   SMR; Q25540; -.
DR   VEuPathDB; ToxoDB:Ncaninum_LIV_000459700; -.
DR   VEuPathDB; ToxoDB:NCLIV_052880; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR008119; Gra6_protein.
DR   Pfam; PF05084; GRA6; 1.
DR   PIRSF; PIRSF037504; Gra6_protein; 1.
DR   PRINTS; PR01746; DENSEGRNULE6.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..193
FT                   /note="Dense granule protein 2"
FT                   /id="PRO_0000087575"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        153..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          75..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          174..193
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        99..122
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        175..193
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        4
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        74
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        67..70
FT                   /note="GTSE -> NSAR (in Ref. 2; AAC47097)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   193 AA;  20590 MW;  7EF46F94C289BC0B CRC64;
     MANNRTLARR RRAFSPLTVV MLAVTLVAFM GVPLSSTGAA DAADPVESVE ANRRGYTSYG
     EPPVAVGTSE EYVNSSELAG SRDKGNAEAE EEAAEVETDV QPSSVTIDTE ERAAPSQVQV
     QQERMEEADD APKPVPVRSA VPSTVAKRQQ ARHRVIGTAV IAAVVAALLW KFSRRRSGAP
     REGGENENGG EEK