GRA2_TOXGO
ID GRA2_TOXGO Reviewed; 185 AA.
AC P13404;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Dense granule protein 2;
DE Short=Protein GRA 2;
DE AltName: Full=28 kDa antigen;
DE AltName: Full=GP28.5;
DE Flags: Precursor;
GN Name=GRA2;
OS Toxoplasma gondii.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX NCBI_TaxID=5811;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2710166; DOI=10.1016/0166-6851(89)90014-5;
RA Prince J.B., Araujo F.G., Remington J.S., Burg J.L., Boothroyd J.C.,
RA Sharma S.D.;
RT "Cloning of cDNAs encoding a 28 kilodalton antigen of Toxoplasma gondii.";
RL Mol. Biochem. Parasitol. 34:3-13(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION TO N-TERMINUS.
RC STRAIN=RH;
RX PubMed=8426609; DOI=10.1016/0166-6851(93)90253-t;
RA Parmley S.F., Sgarlato G.D., Remington J.S.;
RT "Genomic and corrected cDNA sequence of the P28 gene from Toxoplasma
RT gondii.";
RL Mol. Biochem. Parasitol. 57:161-165(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=RH;
RX PubMed=8384696; DOI=10.1016/0166-6851(93)90092-c;
RA Mercier C., Lecordier L., Darcy F., Deslee D., Murray A., Tourvieille B.,
RA Maes P., Capron A., Cesbron-Delauw M.-F.;
RT "Molecular characterization of a dense granule antigen (Gra 2) associated
RT with the network of the parasitophorous vacuole in Toxoplasma gondii.";
RL Mol. Biochem. Parasitol. 58:71-82(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 128-185.
RX PubMed=1383696; DOI=10.1016/0161-5890(92)90174-v;
RA Cesbron-Delauw M.-F., Boutillon C., Mercier C., Fourmaux M.P., Murray A.,
RA Miquey F., Tartar A., Capron A.;
RT "Amino acid sequence requirements for the epitope recognized by a
RT monoclonal antibody reacting with the secreted antigen GP28.5 of Toxoplasma
RT gondii.";
RL Mol. Immunol. 29:1375-1382(1992).
CC -!- FUNCTION: Major granular component involved in excreted-secreted
CC antigen (ESA) immunity. Possibly acts in conjunction with GRA1.
CC -!- SUBCELLULAR LOCATION: Parasitophorous vacuole. Note=Located in dense
CC granules of tachyzoites. Upon infection, secreted, in a Ca(2+)-
CC dependent manner, into the parasitophorous vacuole (PV) and targeted to
CC the microvillus membranous network.
CC -!- PTM: May also be O-glycosylated.
CC -!- PTM: The N-terminus is blocked.
CC -!- MISCELLANEOUS: This protein is one of the antigens specifically
CC recognized by patients infected by Toxoplasma gondii. Possible
CC candidate for an effective vaccine - of special value for
CC immunocompromised patients such as those with AIDS or neoplastic
CC disease.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA30138.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; J04018; AAA30138.1; ALT_INIT; Genomic_DNA.
DR EMBL; M99392; AAB59210.1; -; Genomic_DNA.
DR PIR; A48568; A48568.
DR AlphaFoldDB; P13404; -.
DR VEuPathDB; ToxoDB:TGARI_227620; -.
DR VEuPathDB; ToxoDB:TGCAST_227620; -.
DR VEuPathDB; ToxoDB:TGCOUG_227620; -.
DR VEuPathDB; ToxoDB:TGDOM2_227620; -.
DR VEuPathDB; ToxoDB:TGFOU_227620; -.
DR VEuPathDB; ToxoDB:TGGT1_227620; -.
DR VEuPathDB; ToxoDB:TGMAS_227620; -.
DR VEuPathDB; ToxoDB:TGME49_227620; -.
DR VEuPathDB; ToxoDB:TGP89_227620; -.
DR VEuPathDB; ToxoDB:TGPRC2_227620; -.
DR VEuPathDB; ToxoDB:TGRH88_047040; -.
DR VEuPathDB; ToxoDB:TGRUB_227620; -.
DR VEuPathDB; ToxoDB:TGVAND_227620; -.
DR VEuPathDB; ToxoDB:TGVEG_227620; -.
DR GO; GO:0020003; C:symbiont-containing vacuole; IEA:UniProtKB-SubCell.
DR InterPro; IPR008118; Gra2_protein.
DR PIRSF; PIRSF007972; Gra2_protein; 1.
DR PRINTS; PR01745; DENSEGRNULE2.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..185
FT /note="Dense granule protein 2"
FT /id="PRO_0000021366"
FT REGION 41..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 142..185
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..70
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..160
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 18
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 185 AA; 19843 MW; CF3471FDAF12C665 CRC64;
MFAVKHCLLV VAVGALVNVS VRAAEFSGVV NQGPVDVPFS GKPLDERAVG GKGEHTPPLP
DERQQEPEEP VSQRASRVAE QLFRKFLKFA ENVGHHSEKA FKKAKVVAEK GFTAAKTHTV
RGFKVAKEAA GRGMVTVGKK LANVESDRST TTTQAPDSPN GLAETEVPVE PQQRAAHVPV
PDFSQ
