ID   GRA3_POGPU              Reviewed;          25 AA.
AC   P69847;
DT   24-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Grammistin Pp 3;
OS   Pogonoperca punctata (Clown grouper) (Grammistes punctatus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Serranoidei; Serranidae; Epinephelinae;
OC   Grammistini; Pogonoperca.
OX   NCBI_TaxID=160738;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin secretion;
RX   DOI=10.1046/j.1444-2906.2001.00213.x;
RA   Shiomi K., Yokota H., Nagashima Y., Ishida M.;
RT   "Primary and secondary structures of grammistins, peptide toxins isolated
RT   from the skin secretion of the soapfish Pogonoperca punctata.";
RL   Fish. Sci. 67:163-169(2001).
RN   [2]
RP   FUNCTION.
RX   DOI=10.1046/j.1444-2906.2001.00343.x;
RA   Yokota H., Nagashima Y., Shiomi K.;
RT   "Interaction of grammistins with lipids and their antibacterial activity.";
RL   Fish. Sci. 67:928-933(2001).
CC   -!- FUNCTION: Thanks to its abundant amphiphilic alpha-helices, it may
CC       integrate into membrane phospholipids, leading to lysis of the
CC       membrane. Has hemolytic activity. Has antibacterial activity with a
CC       broad spectrum against various species of bacteria including both Gram-
CC       positive and Gram-negative groups. Has also ichthyotoxic activity.
CC       {ECO:0000269|Ref.2}.
CC   -!- SUBUNIT: Exists as aggregates of 3-4 molecules. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands. {ECO:0000305|Ref.1}.
CC   -!- MASS SPECTROMETRY: Mass=2585; Method=MALDI;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- TOXIC DOSE: LD(50) is 20-40 ug/ml against killifish.
CC   -!- SIMILARITY: Belongs to the grammistin family. Group 3 subfamily.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P69847; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing; Hemolysis;
KW   Secreted; Toxin.
FT   PEPTIDE         1..25
FT                   /note="Grammistin Pp 3"
FT                   /id="PRO_0000044530"
SQ   SEQUENCE   25 AA;  2587 MW;  CD2CEE65C6180F94 CRC64;
     NWRKILGQIA SVGAGLLGSL LAGYE