GRA4_TOXGO
ID GRA4_TOXGO Reviewed; 345 AA.
AC Q27002; O15889; Q03883;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Dense granule protein 4;
DE Short=Protein GRA 4;
DE AltName: Full=Antigen H11;
DE Flags: Precursor;
GN Name=GRA4; Synonyms=H11;
OS Toxoplasma gondii.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX NCBI_TaxID=5811;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RH;
RX PubMed=1362450; DOI=10.1016/0166-6851(92)90172-g;
RA Mevelec M.-N., Chardes T., Mercereau-Puijalon O., Bourguin I., Achbarou A.,
RA Dubremetz J.-F., Bout D.;
RT "Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein,
RT recognized by mucosal IgA antibodies.";
RL Mol. Biochem. Parasitol. 56:227-238(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 290-345.
RC STRAIN=RH;
RX PubMed=2022319; DOI=10.1016/0378-1119(91)90044-c;
RA Johnson A.M., Illana S.;
RT "Cloning of Toxoplasma gondii gene fragments encoding diagnostic
RT antigens.";
RL Gene 99:127-132(1991).
CC -!- FUNCTION: Major granular component involved in excreted-secreted
CC antigen (ESA) immunity.
CC -!- SUBCELLULAR LOCATION: Secreted. Parasitophorous vacuole lumen.
CC Parasitophorous vacuole membrane. Cytoplasmic vesicle, secretory
CC vesicle. Note=Located in dense granules of tachyzoites. Upon infection,
CC secreted into the parasitophorous vacuole (PV) and targeted to the
CC microvillus membranous network.
CC -!- PTM: O-glycosylated. {ECO:0000305}.
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DR EMBL; M76432; AAA30142.1; -; Genomic_DNA.
DR EMBL; M57303; AAA30137.1; -; mRNA.
DR PIR; A48462; A48462.
DR PIR; PS0424; PS0424.
DR AlphaFoldDB; Q27002; -.
DR VEuPathDB; ToxoDB:TGARI_310780; -.
DR VEuPathDB; ToxoDB:TGCAST_310780; -.
DR VEuPathDB; ToxoDB:TGCOUG_310780; -.
DR VEuPathDB; ToxoDB:TGDOM2_310780; -.
DR VEuPathDB; ToxoDB:TGFOU_310780; -.
DR VEuPathDB; ToxoDB:TGGT1_310780; -.
DR VEuPathDB; ToxoDB:TGMAS_310780; -.
DR VEuPathDB; ToxoDB:TGME49_310780; -.
DR VEuPathDB; ToxoDB:TGP89_310780; -.
DR VEuPathDB; ToxoDB:TGPRC2_310780; -.
DR VEuPathDB; ToxoDB:TGRH88_050290; -.
DR VEuPathDB; ToxoDB:TGRUB_310780; -.
DR VEuPathDB; ToxoDB:TGVAND_310780; -.
DR VEuPathDB; ToxoDB:TGVEG_310780; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020005; C:symbiont-containing vacuole membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030133; C:transport vesicle; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Glycoprotein; Membrane; Secreted; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..345
FT /note="Dense granule protein 4"
FT /id="PRO_0000021369"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 227..271
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 227..255
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 290..292
FT /note="AAA -> EFP (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 345 AA; 36284 MW; AC64DD1EE8E58569 CRC64;
MQGTWFSLFV VVMVSHLACG GECSFGSHLA DMAGLSGRHD KERHQAKKRY YHSMYGNQTP
YPYANGQQAS PPPQGQLLII QNPDGSFMIV DQQGVPQVPQ AAGGPGSPMN GGYYMPTGVY
TAQVVPGTPG HPVQAIPQQP LRTQATATYY HPAAVPPPGP SVFVFTPSSV QPGAEVTPGY
SGLQLRQQSQ YGYSYPGTTS TPTPPPPASY GYPVFPAFPR LPAFSDSVSV STEDSGLTGV
KDSSSSESTV TPADEAASES EEGDKTSRKS KVKKGILTGL GVAATLAAAA AAAKAVKGFG
GTRTSTAPAE AGKTELDDGY RPPPFNPRPS PYAELLKDLE RMRKE
