ID   GRA5_GIBZE              Reviewed;         333 AA.
AC   I1R9A8; A0A098D0A1;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2012, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Gramillins biosynthetic cluster protein FGSG_00039 {ECO:0000303|PubMed:30395461};
GN   ORFNames=FG00039, FGRAMPH1_01T00135, FGSG_00039;
OS   Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS   / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX   NCBI_TaxID=229533;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=17823352; DOI=10.1126/science.1143708;
RA   Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA   Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA   Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA   Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA   Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA   Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA   Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA   Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT   "The Fusarium graminearum genome reveals a link between localized
RT   polymorphism and pathogen specialization.";
RL   Science 317:1400-1402(2007).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=20237561; DOI=10.1038/nature08850;
RA   Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA   Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA   Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA   Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA   Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA   Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA   Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA   Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA   Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA   Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA   Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA   Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT   "Comparative genomics reveals mobile pathogenicity chromosomes in
RT   Fusarium.";
RL   Nature 464:367-373(2010).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA   King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA   Hammond-Kosack K.E.;
RT   "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT   graminearum.";
RL   BMC Genomics 16:544-544(2015).
RN   [4]
RP   FUNCTION, AND PATHWAY.
RX   PubMed=30395461; DOI=10.1021/jacs.8b10017;
RA   Bahadoor A., Brauer E.K., Bosnich W., Schneiderman D., Johnston A.,
RA   Aubin Y., Blackwell B., Melanson J.E., Harris L.J.;
RT   "Gramillin A and B: cyclic lipopeptides identified as the nonribosomal
RT   biosynthetic products of Fusarium graminearum.";
RL   J. Am. Chem. Soc. 140:16783-16791(2018).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       gramillins A and B, bicyclic lipopeptides that induce cell death in
CC       maize leaves but not in wheat leaves (PubMed:30395461). The
CC       nonribosomal peptide synthetase GRA1 incorporates respectively a
CC       glutamic adic (Glu), a leucine (Leu), a serine (Ser), a
CC       hydroxyglutamine (HOGln), a 2-amino decanoic acid, and 2 cysteins (CysB
CC       and CysA) (Probable). The biosynthesis of 2-amino decanoic acid
CC       incorporated in gramillins could be initiated by a fatty acid synthase
CC       composed of the alpha and beta subunits FGSG_00036 and FGSG_11656
CC       (Probable). The cytochrome P450 monooxygenase FGSG_15680 could
CC       hydroxylate the fatty acid chain (Probable). Subsequent oxidation to
CC       the ketone by the oxidoreductase FGSG_00048 and transamination by
CC       aminotransferase FGSG_00049 could form 2-amino-decanoic acid
CC       (Probable). On the other hand, FGSG_15680 could also be responsible for
CC       the HO-modified glutamine at the gamma-position (Probable). Whether
CC       hydroxylation occurs on the fully assembled product or on the Gln
CC       residue prior to assembly into the gramillins requires further proof
CC       (Probable). The thioredoxin FGSG_00043 could also be required for the
CC       disulfide-bond formation between CysA and CysB (Probable). The specific
CC       involvement of the remaining proteins from the cluster is more
CC       difficult to discern, but could have broader regulatory (FGSG_00040 and
CC       FGSG_11657) or enzymatic functions (FGSG_00044 and FGSG_00045)
CC       (Probable). The final C-domain of GRA1 does not possess the expected
CC       sequence of a termination CT domain, often implicated in
CC       macrocyclization and release of a cyclopeptidein fungal NRPs; and the
CC       thioesterase FGSG_00047 may act in concert with the terminal C-domain
CC       of GRA1 to catalyze the formation of the macrocyclic anhydride and
CC       release of the products (Probable). {ECO:0000269|PubMed:30395461,
CC       ECO:0000305|PubMed:30395461}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000305|PubMed:30395461}.
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DR   EMBL; HG970332; CEF71867.1; -; Genomic_DNA.
DR   RefSeq; XP_011315626.1; XM_011317324.1.
DR   AlphaFoldDB; I1R9A8; -.
DR   GeneID; 23547557; -.
DR   KEGG; fgr:FGSG_00039; -.
DR   VEuPathDB; FungiDB:FGRAMPH1_01G00135; -.
DR   eggNOG; ENOG502SEJU; Eukaryota.
DR   HOGENOM; CLU_059589_0_0_1; -.
DR   InParanoid; I1R9A8; -.
DR   Proteomes; UP000070720; Chromosome 1.
PE   4: Predicted;
KW   Reference proteome; Virulence.
FT   CHAIN           1..333
FT                   /note="Gramillins biosynthetic cluster protein FGSG_00039"
FT                   /id="PRO_0000450570"
SQ   SEQUENCE   333 AA;  37215 MW;  2C483F6806470600 CRC64;
     MHLDHKLPWK TIASHFTLAR DGSSPNYNLI ALGLSTQDAA LGHFSRIFIA TIEEFSNTET
     SKINLNQVDG KLFSDDVLNF PEHHFGLGPH DTNSALNNPL DAKHQELKYW KGRASTASEY
     GTSPEYSASY STADGDLADA AKMLVITAAV SDNQAIRREA LSALCQLSAH VPMSDLRGLS
     WGHGFGLSLV ASEALKLYML LNLIEAVQSR GAQQVSLLRV KILLCALGNY SLQNYDFPAQ
     NIPHRAFWHS LGITESWADR QADGVRESDE PVVDPLGLGD DEIHQEARIN LKKYLKDCFA
     ILYVYDVFLK QIASEEERKE FWSYEINRVI LYL