GRA6_TOXGO
ID GRA6_TOXGO Reviewed; 230 AA.
AC Q27003; Q27009; Q969D5; Q9N6E2; Q9N6R8; Q9NGV5; Q9NGV6; Q9NGV7; Q9NGV8;
AC Q9NGV9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2003, sequence version 2.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Dense granule protein 6;
DE Short=Protein GRA 6;
DE AltName: Full=Antigen p32;
DE AltName: Full=Protein p33;
GN Name=GRA6; Synonyms=TG24;
OS Toxoplasma gondii.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX NCBI_TaxID=5811;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RH;
RX PubMed=7637717; DOI=10.1016/0166-6851(95)00010-x;
RA Lecordier L., Moleon-Borodowsky I., Dubremetz J.-F., Tourvieille B.,
RA Mercier C., Deslee D., Capron A., Cesbron-Delauw M.-F.;
RT "Characterization of a dense granule antigen of Toxoplasma gondii (GRA6)
RT associated to the network of the parasitophorous vacuole.";
RL Mol. Biochem. Parasitol. 70:85-94(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RH;
RA Becker K.;
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 50611 / Me49, ATCC 50859 / M7741, ATCC 50869 / FOU,
RC ATCC 50870 / MAS, Beverley, Castells, NED, RH, RUB, and TONT;
RX PubMed=10779578; DOI=10.1016/s0020-7519(00)00036-9;
RA Fazaeli A., Carter P.E., Darde M.L., Pennington T.H.;
RT "Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence
RT analysis.";
RL Int. J. Parasitol. 30:637-642(2000).
CC -!- FUNCTION: Major granular component involved in excreted-secreted
CC antigen (ESA) immunity. May have a structural role in the membranous
CC network of the parasitophorous vacuole (PV).
CC -!- SUBCELLULAR LOCATION: Secreted. Parasitophorous vacuole lumen.
CC Parasitophorous vacuole membrane. Cytoplasmic vesicle, secretory
CC vesicle. Note=Located in dense granules of tachyzoites. Upon infection,
CC secreted into the parasitophorous vacuole (PV) and targeted to the
CC microvillus membranous network.
CC -!- SIMILARITY: Belongs to the Gra6 family. {ECO:0000305}.
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DR EMBL; L33814; AAC37235.1; -; Genomic_DNA.
DR EMBL; X96720; CAA65481.1; -; Genomic_DNA.
DR EMBL; AF239283; AAF60334.1; -; Genomic_DNA.
DR EMBL; AF239284; AAF60335.1; -; Genomic_DNA.
DR EMBL; AF239285; AAF60336.1; -; Genomic_DNA.
DR EMBL; AF239286; AAF60337.1; -; Genomic_DNA.
DR EMBL; AF239287; AAF60338.1; -; Genomic_DNA.
DR EMBL; AF239288; AAF60339.1; -; Genomic_DNA.
DR EMBL; AF239289; AAF60340.1; -; Genomic_DNA.
DR EMBL; AF239290; AAF60341.1; -; Genomic_DNA.
DR EMBL; AF239291; AAF60342.1; -; Genomic_DNA.
DR EMBL; AF239292; AAF60343.1; -; Genomic_DNA.
DR AlphaFoldDB; Q27003; -.
DR TCDB; 9.B.11.1.1; the dense granule protein 6 (gra6) family.
DR VEuPathDB; ToxoDB:TGARI_275440; -.
DR VEuPathDB; ToxoDB:TGCAST_275440; -.
DR VEuPathDB; ToxoDB:TGCOUG_275440; -.
DR VEuPathDB; ToxoDB:TGDOM2_275440; -.
DR VEuPathDB; ToxoDB:TGFOU_275440; -.
DR VEuPathDB; ToxoDB:TGGT1_275440; -.
DR VEuPathDB; ToxoDB:TGMAS_275440; -.
DR VEuPathDB; ToxoDB:TGME49_275440; -.
DR VEuPathDB; ToxoDB:TGP89_275440; -.
DR VEuPathDB; ToxoDB:TGPRC2_275440; -.
DR VEuPathDB; ToxoDB:TGRH88_038710; -.
DR VEuPathDB; ToxoDB:TGRUB_275440; -.
DR VEuPathDB; ToxoDB:TGVAND_275440; -.
DR VEuPathDB; ToxoDB:TGVEG_275440; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020005; C:symbiont-containing vacuole membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030133; C:transport vesicle; IEA:UniProtKB-SubCell.
DR InterPro; IPR008119; Gra6_protein.
DR Pfam; PF05084; GRA6; 1.
DR PIRSF; PIRSF037504; Gra6_protein; 1.
DR PRINTS; PR01746; DENSEGRNULE6.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle; Glycoprotein; Membrane; Secreted; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..230
FT /note="Dense granule protein 6"
FT /id="PRO_0000087576"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 46..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 175..230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 114..131
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..230
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 7
FT /note="H -> Y (in strain: Beverley, MAS and Me49)"
FT VARIANT 17
FT /note="V -> L (in strain: Castells, FOU, MAS, NED, RUB and
FT TONT)"
FT VARIANT 47
FT /note="G -> D (in strain: Castells, NED and TONT)"
FT VARIANT 50
FT /note="K -> Q (in strain: FOU)"
FT VARIANT 50
FT /note="K -> R (in strain: Beverley and Me49)"
FT VARIANT 58
FT /note="S -> L (in strain: RUB)"
FT VARIANT 94
FT /note="E -> D (in strain: Beverley, Castells, FOU, MAS,
FT Me49, NED, RUB and TONT)"
FT VARIANT 105
FT /note="V -> A (in strain: Beverley, MAS and Me49)"
FT VARIANT 180
FT /note="Q -> P (in strain: Castells, FOU, NED, RUB and
FT TONT)"
FT VARIANT 185
FT /note="D -> G (in strain: Beverley, MAS and Me49)"
FT VARIANT 185
FT /note="D -> H (in strain: RUB)"
FT VARIANT 198
FT /note="G -> R (in strain: M7741, NED and TONT)"
FT VARIANT 205..209
FT /note="Missing (in strain: Beverley and Me49)"
FT VARIANT 206
FT /note="G -> R (in strain: Castells and FOU)"
FT VARIANT 213
FT /note="A -> G (in strain: Castells, FOU, M7741, NED, RUB
FT and TONT)"
FT VARIANT 213
FT /note="Missing (in strain: Beverley, MAS and Me49)"
FT VARIANT 216
FT /note="D -> G (in strain: MAS)"
FT VARIANT 217
FT /note="R -> G (in strain: FOU)"
FT VARIANT 218
FT /note="R -> G (in strain: MAS)"
FT VARIANT 219
FT /note="P -> A (in strain: Castells, FOU, M7741, NED, RUB
FT and TONT)"
FT VARIANT 222
FT /note="P -> L (in strain: RUB)"
FT VARIANT 223
FT /note="E -> G (in strain: Beverley, Castells, MAS and
FT Me49)"
FT VARIANT 224
FT /note="R -> S (in strain: Beverley, Castells, MAS and
FT Me49)"
FT VARIANT 227
FT /note="V -> A (in strain: RUB)"
FT VARIANT 227
FT /note="V -> E (in strain: Beverley, MAS and Me49)"
FT VARIANT 230
FT /note="Y -> F (in strain: Beverley, MAS and Me49)"
FT CONFLICT 41
FT /note="A -> R (in Ref. 1; AAC37235)"
FT /evidence="ECO:0000305"
FT CONFLICT 144
FT /note="S -> C (in Ref. 1; AAC37235)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 230 AA; 24029 MW; 6EE6F8B0D6F7F7B5 CRC64;
MAHGGIHLRQ KRNFCPVTVS TVAVVFVVFM GVLVNSLGGV AVAADSGGVK QTPSETGSSG
GQQEAVGTTE DYVNSSAMGG GQGDSLAEDD TTSEAAEGDV DPFPVLANEG KSEARGPSLE
ERIEEQGTRR RYSSVQEPQA KVPSKRTQKR HRLIGAVVLA VSVAMLTAFF LRRTGRRSPQ
EPSGDGGGND AGNNAGNGGN EGRGYGGRGE GGAEDDRRPL HPERVNVFDY
