ID   GRAD_RHIS5              Reviewed;         179 AA.
AC   A1IIX5;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   23-FEB-2022, entry version 34.
DE   RecName: Full=FADH(2)-dependent resorcinol hydroxylase, reductase component {ECO:0000305};
DE            EC=1.5.1.37 {ECO:0000269|PubMed:17158677};
GN   Name=graD {ECO:0000303|PubMed:17158677};
OS   Rhizobium sp. (strain MTP-10005).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=267998;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT,
RP   AND INDUCTION.
RC   STRAIN=MTP-10005;
RX   PubMed=17158677; DOI=10.1128/jb.01675-06;
RA   Yoshida M., Oikawa T., Obata H., Abe K., Mihara H., Esaki N.;
RT   "Biochemical and genetic analysis of the gamma-resorcylate (2,6-
RT   dihydroxybenzoate) catabolic pathway in Rhizobium sp. strain MTP-10005:
RT   identification and functional analysis of its gene cluster.";
RL   J. Bacteriol. 189:1573-1581(2007).
CC   -!- FUNCTION: Involved in the gamma-resorcylate (2,6-dihydroxybenzoate)
CC       catabolism (PubMed:17158677). Reductase component of the resorcinol
CC       hydroxylase, which catalyzes the FADPH-dependent conversion of
CC       resorcinol to hydroxyquinol (PubMed:17158677). Catalyzes the reduction
CC       of FAD by NADH. The reduced flavin is then transferred to the oxygenase
CC       component GraA (PubMed:17158677). {ECO:0000269|PubMed:17158677}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=FADH2 + NAD(+) = FAD + 2 H(+) + NADH; Xref=Rhea:RHEA:30147,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57692,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:58307; EC=1.5.1.37;
CC         Evidence={ECO:0000269|PubMed:17158677};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:30149;
CC         Evidence={ECO:0000269|PubMed:17158677};
CC   -!- PATHWAY: Aromatic compound metabolism. {ECO:0000305}.
CC   -!- SUBUNIT: The FADH(2)-dependent resorcinol hydroxylase is composed of
CC       two subunits, GraA (the oxygenase component) and GraD (the reductase
CC       component). Both subunits are required for activity.
CC       {ECO:0000269|PubMed:17158677}.
CC   -!- INDUCTION: Induced in the presence of gamma-resorcylate.
CC       {ECO:0000269|PubMed:17158677}.
CC   -!- SIMILARITY: Belongs to the non-flavoprotein flavin reductase family.
CC       {ECO:0000305}.
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DR   EMBL; AB266213; BAF44525.1; -; Genomic_DNA.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0016646; F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor; IEA:UniProt.
DR   Gene3D; 2.30.110.10; -; 1.
DR   InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR   InterPro; IPR012349; Split_barrel_FMN-bd.
DR   Pfam; PF01613; Flavin_Reduct; 1.
DR   SMART; SM00903; Flavin_Reduct; 1.
PE   1: Evidence at protein level;
KW   FAD; Flavoprotein; NAD; Oxidoreductase.
FT   CHAIN           1..179
FT                   /note="FADH(2)-dependent resorcinol hydroxylase, reductase
FT                   component"
FT                   /id="PRO_0000454490"
SQ   SEQUENCE   179 AA;  19429 MW;  ECB4E91C7C9A130E CRC64;
     MTSALFGLNN LAPEGVGQNF RTTMRRFPAT VTVITACATG DQRDHGMTVT AVTSVSMEPP
     SLLVCLNNRT FLHELLLCRP DFIVNVLTQD QIALSDAFSG KVSPEERFRN GEWQRHDNGV
     LYLPTAHAAI ACRRVAAMPY GTHTVFIGQV VSADVSETTR PLLYENAQYC AASPAGLSA