ID   GRAK_MOUSE              Reviewed;         263 AA.
AC   O35205;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Granzyme K;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=Gzmk;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/SvJ;
RX   PubMed=9242702; DOI=10.1074/jbc.272.32.20236;
RA   Shresta S., Goda P., Wesselschmidt R., Ley T.J.;
RT   "Residual cytotoxicity and granzyme K expression in granzyme A-deficient
RT   cytotoxic lymphocytes.";
RL   J. Biol. Chem. 272:20236-20244(1997).
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic granule.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Granzyme subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR   EMBL; AF011446; AAC17930.1; -; Genomic_DNA.
DR   CCDS; CCDS26783.1; -.
DR   RefSeq; NP_032222.1; NM_008196.1.
DR   AlphaFoldDB; O35205; -.
DR   SMR; O35205; -.
DR   STRING; 10090.ENSMUSP00000044512; -.
DR   MEROPS; S01.146; -.
DR   iPTMnet; O35205; -.
DR   PhosphoSitePlus; O35205; -.
DR   PaxDb; O35205; -.
DR   PRIDE; O35205; -.
DR   ProteomicsDB; 271157; -.
DR   Antibodypedia; 11044; 309 antibodies from 32 providers.
DR   DNASU; 14945; -.
DR   Ensembl; ENSMUST00000038212; ENSMUSP00000044512; ENSMUSG00000042385.
DR   GeneID; 14945; -.
DR   KEGG; mmu:14945; -.
DR   UCSC; uc007rxd.1; mouse.
DR   CTD; 3003; -.
DR   MGI; MGI:1298232; Gzmk.
DR   VEuPathDB; HostDB:ENSMUSG00000042385; -.
DR   eggNOG; KOG3627; Eukaryota.
DR   GeneTree; ENSGT00940000161886; -.
DR   HOGENOM; CLU_006842_1_0_1; -.
DR   InParanoid; O35205; -.
DR   OMA; KYQAWIK; -.
DR   OrthoDB; 1144875at2759; -.
DR   PhylomeDB; O35205; -.
DR   TreeFam; TF333630; -.
DR   BRENDA; 3.4.21.B4; 3474.
DR   BioGRID-ORCS; 14945; 6 hits in 72 CRISPR screens.
DR   PRO; PR:O35205; -.
DR   Proteomes; UP000000589; Chromosome 13.
DR   RNAct; O35205; protein.
DR   Bgee; ENSMUSG00000042385; Expressed in presomitic mesoderm and 39 other tissues.
DR   ExpressionAtlas; O35205; baseline and differential.
DR   Genevisible; O35205; MM.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; ISO:MGI.
DR   GO; GO:0008236; F:serine-type peptidase activity; ISO:MGI.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Hydrolase; Protease; Reference proteome; Serine protease;
KW   Signal; Zymogen.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..25
FT                   /note="Activation peptide"
FT                   /id="PRO_0000027417"
FT   CHAIN           26..263
FT                   /note="Granzyme K"
FT                   /id="PRO_0000027418"
FT   DOMAIN          26..258
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        66
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        115
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        213
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   DISULFID        51..67
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        148..219
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        180..198
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        209..233
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ   SEQUENCE   263 AA;  29251 MW;  81951594F9AF08DE CRC64;
     MRFSSWALVS LVAGVYMSSE CFHTEIIGGR EVQPHSRPFM ASIQYRSKHI CGGVLIHPQW
     VLTAAHCYSW FPRGHSPTVV LGAHSLSKNE PMKQTFEIKK FIPFSRLQSG SASHDIMLIK
     LRTAAELNKN VQLLHLGSKN YLRDGTKCQV TGWGTTKPDL LTASDTLREV TVTIISRKRC
     NSQSYYNHKP VITKDMICAG DARGQKDSCK GDSGGPLICK GIFHALVSQG YKCGIAKKPG
     IYTLLTKKYQ TWIKSKLAPS RAH