GRAM4_MOUSE
ID GRAM4_MOUSE Reviewed; 633 AA.
AC Q8CB44; B2RTC1; Q6ZQ52; Q8VDL6;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=GRAM domain-containing protein 4 {ECO:0000305};
DE AltName: Full=Death-inducing protein;
GN Name=Gramd4 {ECO:0000312|MGI:MGI:2676308}; Synonyms=Dip, Kiaa0767;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Vagina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 57-633.
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, INTERACTION WITH RTN4, AND SUBCELLULAR LOCATION.
RX PubMed=25917084; DOI=10.4049/jimmunol.1402006;
RA Kimura T., Endo S., Inui M., Saitoh S., Miyake K., Takai T.;
RT "Endoplasmic Protein Nogo-B (RTN4-B) Interacts with GRAMD4 and Regulates
RT TLR9-Mediated Innate Immune Responses.";
RL J. Immunol. 194:5426-5436(2015).
CC -!- FUNCTION: Plays a role as a mediator of E2F1-induced apoptosis in the
CC absence of p53/TP53 (By similarity). Inhibits TLR9 response to nucelic
CC acids and regulates TLR9-mediated innate immune response
CC (PubMed:25917084). {ECO:0000250|UniProtKB:Q6IC98,
CC ECO:0000269|PubMed:25917084}.
CC -!- SUBUNIT: Interacts with RTN4 (isoform B).
CC {ECO:0000269|PubMed:25917084}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000250|UniProtKB:Q6IC98}; Multi-pass membrane protein
CC {ECO:0000255}. Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:25917084}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Colocalizes with COX4I1. {ECO:0000250}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-52 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH21523.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC98019.1; Type=Miscellaneous discrepancy; Note=The sequence differs from that shown because it seems to be derived from a pre-mRNA.; Evidence={ECO:0000305};
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DR EMBL; AK036832; BAC29597.1; -; mRNA.
DR EMBL; AK129209; BAC98019.1; ALT_SEQ; Transcribed_RNA.
DR EMBL; BC021523; AAH21523.1; ALT_INIT; mRNA.
DR EMBL; BC139228; AAI39229.1; -; mRNA.
DR EMBL; BC139229; AAI39230.1; -; mRNA.
DR RefSeq; NP_766199.1; NM_172611.4.
DR AlphaFoldDB; Q8CB44; -.
DR SMR; Q8CB44; -.
DR BioGRID; 230188; 1.
DR STRING; 10090.ENSMUSP00000086321; -.
DR iPTMnet; Q8CB44; -.
DR PhosphoSitePlus; Q8CB44; -.
DR EPD; Q8CB44; -.
DR MaxQB; Q8CB44; -.
DR PaxDb; Q8CB44; -.
DR PeptideAtlas; Q8CB44; -.
DR PRIDE; Q8CB44; -.
DR ProteomicsDB; 271324; -.
DR Antibodypedia; 28118; 52 antibodies from 13 providers.
DR Ensembl; ENSMUST00000088931; ENSMUSP00000086321; ENSMUSG00000035900.
DR GeneID; 223752; -.
DR KEGG; mmu:223752; -.
DR UCSC; uc007xdv.3; mouse.
DR CTD; 23151; -.
DR MGI; MGI:2676308; Gramd4.
DR VEuPathDB; HostDB:ENSMUSG00000035900; -.
DR eggNOG; ENOG502QPMR; Eukaryota.
DR GeneTree; ENSGT00390000010968; -.
DR HOGENOM; CLU_028241_0_0_1; -.
DR InParanoid; Q8CB44; -.
DR OMA; XYLCFES; -.
DR OrthoDB; 1148554at2759; -.
DR PhylomeDB; Q8CB44; -.
DR TreeFam; TF320445; -.
DR BioGRID-ORCS; 223752; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Gramd4; mouse.
DR PRO; PR:Q8CB44; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8CB44; protein.
DR Bgee; ENSMUSG00000035900; Expressed in interventricular septum and 244 other tissues.
DR ExpressionAtlas; Q8CB44; baseline and differential.
DR Genevisible; Q8CB44; MM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0034164; P:negative regulation of toll-like receptor 9 signaling pathway; IMP:UniProtKB.
DR GO; GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; ISO:MGI.
DR CDD; cd13221; PH-GRAM_GRAMDC4; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR004182; GRAM.
DR InterPro; IPR037847; GRAMDC4.
DR InterPro; IPR037845; GRAMDC4_PH-GRAM.
DR InterPro; IPR011993; PH-like_dom_sf.
DR PANTHER; PTHR37402; PTHR37402; 1.
DR Pfam; PF02893; GRAM; 1.
DR SMART; SM00568; GRAM; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Coiled coil; Endoplasmic reticulum; Membrane; Mitochondrion;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..633
FT /note="GRAM domain-containing protein 4"
FT /id="PRO_0000328743"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 500..578
FT /note="GRAM"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 72..109
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 182..216
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 134..190
FT /evidence="ECO:0000255"
FT COMPBIAS 189..216
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 79
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6IC98"
SQ SEQUENCE 633 AA; 72264 MW; C961FEE9749B3AD3 CRC64;
MGIASHSAFR ERESSPTGAS LDASPRPWDK GLSGREPPRH VQVRPRSAVL NMLRRLDRIR
FRGHKREDLL DLAESPNASD TECGDEIPLK TPRPSPRDSE ELRDPAGPGT LIMAAGVQDF
NRTEFDRLNE IKGHLEIALL EKHFLQEELR KLREETNSEM LRQELDRERQ RRIELEQKMQ
EVLKARSEEQ PAQPQQPPKG QSQASNGTGT ERRSQGLASR VQKWFYERFG EYIEDFRFQP
EENTVETEEP LSARRLTENM RRLKRGAKPV TNFVKNLSAL SDWYSIYTSA IAFTVYMNAV
WHGWAIPMFL FLAILRLSLN YLIARGWRIQ WSIVPEVSEA VEPAKEDLTV SEKFQLVLDV
AQKAQNLFGK MADILEKIKN LFMWVQPETT QKLYVALWAA FLASCFFPYR LVGLAVGLYA
GIKFFLIDFI FKRCPRLRAK YDTPYIIWRS LPTDPQLKER AGATVSRRLQ TASSRSYVSS
APAGLSKDED AGRFHSTKKG NFHEIFNLTE NERPLAVCEN GWRCCLINRD RKMPTDYIRN
GVLYVTENYL CFESSKSGSS KRNKVIKLMD ITDIQKYKVL SVLPGSGMGI AVSTPSTQKP
LVFGAMVHRD EAFETIFSQY VKITSAAASG GDS
