ID   GRAM4_XENLA             Reviewed;         578 AA.
AC   A2RV80;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=GRAM domain-containing protein 4;
GN   Name=gramd4;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role as a mediator of e2f1-induced apoptosis in the
CC       absence of p53/TP53. {ECO:0000250|UniProtKB:Q6IC98}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC       {ECO:0000250|UniProtKB:Q6IC98}; Multi-pass membrane protein
CC       {ECO:0000255}. Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q8CB44}; Multi-pass membrane protein
CC       {ECO:0000255}.
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DR   EMBL; BC133216; AAI33217.1; -; mRNA.
DR   RefSeq; NP_001091321.1; NM_001097852.1.
DR   AlphaFoldDB; A2RV80; -.
DR   SMR; A2RV80; -.
DR   GeneID; 100037148; -.
DR   KEGG; xla:100037148; -.
DR   CTD; 100037148; -.
DR   OrthoDB; 1148554at2759; -.
DR   Proteomes; UP000186698; Chromosome 3S.
DR   Bgee; 100037148; Expressed in blastula and 19 other tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   CDD; cd13221; PH-GRAM_GRAMDC4; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR004182; GRAM.
DR   InterPro; IPR037847; GRAMDC4.
DR   InterPro; IPR037845; GRAMDC4_PH-GRAM.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   PANTHER; PTHR37402; PTHR37402; 1.
DR   Pfam; PF02893; GRAM; 1.
DR   SMART; SM00568; GRAM; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; Coiled coil; Endoplasmic reticulum; Membrane; Mitochondrion;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..578
FT                   /note="GRAM domain-containing protein 4"
FT                   /id="PRO_0000328744"
FT   TRANSMEM        236..256
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        356..376
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          446..524
FT                   /note="GRAM"
FT   REGION          132..157
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          83..135
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        135..157
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   578 AA;  66546 MW;  22F3D39160F4848F CRC64;
     MLKRLDKIRF RGQKRDDFLD LVESPNASDT ECGDEIPMKI PPTSLKDTEE LKDPAGSGTI
     IMASGVAEYN RTESDRLNEV KGHLEIALLE KHFLQEELRK LREETNIDTL KQELEKERQR
     RTELEQKITD IAKTRTDESA TQQLSKGPSQ TNGADKQRSK TMCYRVQKWF YDKFGEYIED
     FRFQPEECTV ETEEPLSARR LTENMRRLKR GAKPVTNFVK NLSALSDWHS VYTSAIAFII
     YMNAVWHGWA IPMFLFLAIL RLSLNYLIAR GWRIQWSIVP QVSETLELPK EDLTVSEKFQ
     LVLDVAQKAQ NLFGKMADIL EKIKNLFMWV QPEMTQKLYI GLWAAFVASC FFHYKTIGLC
     MGLYAGIKFF LIDFIFKRCP RLRAKYDTPY IIWTSLPTDP QLKERTNATS SRRIQTVYSR
     GNLASSAPQG VSRDEETGRF HSTKKSSFHE IFSLLETERP LPACETGWRC CLINRDRKMP
     TDYIRNGILY VTENFLCFES SRSGSSKRNK VIKLTDITDI QKYKVLSVLP GSGMGIAVST
     PSTQKPLVFG AMVHRDEAFE TIFSQYVKIT SAVTNSDT