GRAN_MOUSE
ID GRAN_MOUSE Reviewed; 220 AA.
AC Q8VC88; Q8BL53; Q8K3Z6;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Grancalcin;
GN Name=Gca;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DISRUPTION PHENOTYPE.
RX PubMed=12529388; DOI=10.1128/mcb.23.3.826-830.2003;
RA Roes J., Choi B.K., Power D., Xu P., Segal A.W.;
RT "Granulocyte function in grancalcin-deficient mice.";
RL Mol. Cell. Biol. 23:826-830(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=15118320; DOI=10.1271/bbb.68.894;
RA Liu F., Shinomiya H., Kirikae T., Hirata H., Asano Y.;
RT "Characterization of murine grancalcin specifically expressed in leukocytes
RT and its possible role in host defense against bacterial infection.";
RL Biosci. Biotechnol. Biochem. 68:894-902(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cecum, Corpora quadrigemina, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=16934789; DOI=10.1016/j.cellimm.2006.07.004;
RA Xu P., Roes J., Segal A.W., Radulovic M.;
RT "The role of grancalcin in adhesion of neutrophils.";
RL Cell. Immunol. 240:116-121(2006).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Calcium-binding protein that may play a role in the adhesion
CC of neutrophils to fibronectin. May play a role in the formation of
CC focal adhesions. {ECO:0000269|PubMed:16934789}.
CC -!- SUBUNIT: Homodimer. Interacts with SRI and LCP1 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P28676}.
CC Cytoplasmic granule membrane {ECO:0000250|UniProtKB:P28676}; Peripheral
CC membrane protein {ECO:0000250|UniProtKB:P28676}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:P28676}. Note=Primarily cytosolic in the absence
CC of calcium or magnesium ions. Relocates to granules and other membranes
CC in response to elevated calcium and magnesium levels.
CC {ECO:0000250|UniProtKB:P28676}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Not essential for normal
CC resistance to microbial infections. Grancalcin-deficient neutrophils
CC exhibit decreased adhesion to fibronectin, and a strongly reduced
CC number of focal adhesion complexes. {ECO:0000269|PubMed:12529388,
CC ECO:0000269|PubMed:16934789}.
CC -!- MISCELLANEOUS: This protein has been shown to bind calcium with high
CC affinity. {ECO:0000250}.
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DR EMBL; AF518325; AAM66720.1; -; mRNA.
DR EMBL; AB088388; BAC07231.1; -; mRNA.
DR EMBL; AK033740; BAC28458.1; -; mRNA.
DR EMBL; AK046347; BAC32686.1; -; mRNA.
DR EMBL; AK049317; BAC33679.1; -; mRNA.
DR EMBL; AK050540; BAC34315.1; -; mRNA.
DR EMBL; AK078915; BAC37457.1; -; mRNA.
DR EMBL; BC021450; AAH21450.1; -; mRNA.
DR CCDS; CCDS16069.1; -.
DR RefSeq; NP_663498.1; NM_145523.3.
DR AlphaFoldDB; Q8VC88; -.
DR SMR; Q8VC88; -.
DR STRING; 10090.ENSMUSP00000028257; -.
DR PhosphoSitePlus; Q8VC88; -.
DR REPRODUCTION-2DPAGE; IPI00308789; -.
DR MaxQB; Q8VC88; -.
DR PaxDb; Q8VC88; -.
DR PeptideAtlas; Q8VC88; -.
DR PRIDE; Q8VC88; -.
DR ProteomicsDB; 271288; -.
DR Antibodypedia; 33752; 135 antibodies from 26 providers.
DR DNASU; 227960; -.
DR Ensembl; ENSMUST00000028257; ENSMUSP00000028257; ENSMUSG00000026893.
DR GeneID; 227960; -.
DR KEGG; mmu:227960; -.
DR UCSC; uc008jvp.1; mouse.
DR CTD; 25801; -.
DR MGI; MGI:1918521; Gca.
DR VEuPathDB; HostDB:ENSMUSG00000026893; -.
DR eggNOG; KOG0037; Eukaryota.
DR GeneTree; ENSGT00940000153979; -.
DR HOGENOM; CLU_051357_4_0_1; -.
DR InParanoid; Q8VC88; -.
DR OMA; DSMWTYF; -.
DR OrthoDB; 1330600at2759; -.
DR PhylomeDB; Q8VC88; -.
DR TreeFam; TF314682; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 227960; 1 hit in 73 CRISPR screens.
DR PRO; PR:Q8VC88; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8VC88; protein.
DR Bgee; ENSMUSG00000026893; Expressed in granulocyte and 222 other tissues.
DR Genevisible; Q8VC88; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005509; F:calcium ion binding; ISS:UniProtKB.
DR GO; GO:0046982; F:protein heterodimerization activity; ISO:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13833; EF-hand_8; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Calcium; Cytoplasm; Membrane; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..220
FT /note="Grancalcin"
FT /id="PRO_0000073722"
FT DOMAIN 51..86
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 92..127
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 122..157
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 158..193
FT /note="EF-hand 4"
FT /evidence="ECO:0000305"
FT BINDING 105
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 107
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 109
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 111
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 116
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 135
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 137
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 139
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 141
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 146
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 187..188
FT /note="KL -> NV (in Ref. 1; AAM66720)"
FT /evidence="ECO:0000305"
FT CONFLICT 217
FT /note="T -> P (in Ref. 3; BAC32686)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 220 AA; 24663 MW; 694DCA7D6ADF8279 CRC64;
MAYPGYGGAF GNFSGQIPGM QMQMGQPMPG AGPNMFSGGY PGYLGYSDSY SPADDSMWTY
FTAVAGQDGE VDAEELQRCL TQSGISGTYA PFSLETCRIM IAMLDRDYTG KMGFNEFKEL
WAALNAWKQN FMTIDQDQSG TVEHHELSQA IALMGYRLSP QTLAAIVRRY SKNGRIFFDD
YVACCVKLRA LTDFFRRRDH LQQGIVNFMY EDFLQGTMTI
