ID   GRAN_PONAB              Reviewed;         218 AA.
AC   Q5RAI6;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Grancalcin;
GN   Name=GCA;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Calcium-binding protein that may play a role in the adhesion
CC       of neutrophils to fibronectin. May play a role in the formation of
CC       focal adhesions (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Interacts with SRI and LCP1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P28676}.
CC       Cytoplasmic granule membrane {ECO:0000250|UniProtKB:P28676}; Peripheral
CC       membrane protein {ECO:0000250|UniProtKB:P28676}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:P28676}. Note=Primarily cytosolic in the absence
CC       of calcium or magnesium ions. Relocates to granules and other membranes
CC       in response to elevated calcium and magnesium levels.
CC       {ECO:0000250|UniProtKB:P28676}.
CC   -!- MISCELLANEOUS: This protein has been shown to bind calcium with high
CC       affinity. {ECO:0000250}.
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DR   EMBL; CR859029; CAH91224.1; -; mRNA.
DR   RefSeq; NP_001127389.1; NM_001133917.1.
DR   AlphaFoldDB; Q5RAI6; -.
DR   SMR; Q5RAI6; -.
DR   STRING; 9601.ENSPPYP00000014382; -.
DR   PRIDE; Q5RAI6; -.
DR   Ensembl; ENSPPYT00000014968; ENSPPYP00000014382; ENSPPYG00000012888.
DR   GeneID; 100174456; -.
DR   KEGG; pon:100174456; -.
DR   CTD; 25801; -.
DR   eggNOG; KOG0037; Eukaryota.
DR   GeneTree; ENSGT00940000153979; -.
DR   HOGENOM; CLU_051357_4_0_1; -.
DR   InParanoid; Q5RAI6; -.
DR   OMA; DSMWTYF; -.
DR   OrthoDB; 1330600at2759; -.
DR   TreeFam; TF314682; -.
DR   Proteomes; UP000001595; Chromosome 2B.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; ISS:UniProtKB.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13833; EF-hand_8; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 2.
PE   2: Evidence at transcript level;
KW   Calcium; Cytoplasm; Membrane; Metal-binding; Reference proteome; Repeat.
FT   CHAIN           1..218
FT                   /note="Grancalcin"
FT                   /id="PRO_0000073723"
FT   DOMAIN          49..84
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          85..119
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000305"
FT   DOMAIN          120..155
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          156..191
FT                   /note="EF-hand 4"
FT                   /evidence="ECO:0000305"
FT   BINDING         103
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         105
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         107
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         109
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         133
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         135
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         137
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         139
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         144
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ   SEQUENCE   218 AA;  24098 MW;  375A6A99FCA81270 CRC64;
     MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PGILLDGYSG CPAFSDTYSS AGDSVYTYFS
     AVAGQDGEVD AEELQRCLTQ SGISGTYSPF SLETCRIMIA MLDRDYTGKM GFNAFKELWS
     ALNAWKENFM TVDQDGSGTV EHHELRQAIG LMGYRLSPQT LTTIVKRYSK NGRIFFDDYV
     ACCVKLRALT DFFRKRDHLQ QGSANFIYDD FLQGTMAI