ID   GRAP_BOVIN              Reviewed;         217 AA.
AC   A6QLK6;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=GRB2-related adapter protein;
GN   Name=GRAP;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Basal ganglia;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Couples signals from receptor and cytoplasmic tyrosine
CC       kinases to the Ras signaling pathway. Plays a role in the inner ear and
CC       in hearing. {ECO:0000250|UniProtKB:Q13588}.
CC   -!- SUBUNIT: Associates through its SH2 domain with ligand-activated
CC       receptors for stem cell factor (KIT) and erythropoietin (EPOR). Also
CC       forms a stable complex with the Bcr-Abl oncoprotein. GRAP is associated
CC       with the Ras guanine nucleotide exchange factor SOS1, primarily through
CC       its N-terminal SH3 domain. Interacts with phosphorylated LAT upon TCR
CC       activation. Interacts with SHB (By similarity).
CC       {ECO:0000250|UniProtKB:Q13588}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q08012};
CC       Peripheral membrane protein {ECO:0000250|UniProtKB:Q08012}. Synapse
CC       {ECO:0000250|UniProtKB:Q08012}. Note=Localizes at the presynaptic
CC       terminal. {ECO:0000250|UniProtKB:Q08012}.
CC   -!- SIMILARITY: Belongs to the GRB2/sem-5/DRK family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC147998; AAI47999.1; -; mRNA.
DR   RefSeq; NP_001095643.1; NM_001102173.1.
DR   RefSeq; XP_010814339.1; XM_010816037.2.
DR   AlphaFoldDB; A6QLK6; -.
DR   SMR; A6QLK6; -.
DR   STRING; 9913.ENSBTAP00000041340; -.
DR   PaxDb; A6QLK6; -.
DR   Ensembl; ENSBTAT00000043796; ENSBTAP00000041340; ENSBTAG00000003707.
DR   GeneID; 534491; -.
DR   KEGG; bta:534491; -.
DR   CTD; 10750; -.
DR   VEuPathDB; HostDB:ENSBTAG00000003707; -.
DR   eggNOG; KOG3601; Eukaryota.
DR   GeneTree; ENSGT00940000156254; -.
DR   HOGENOM; CLU_073617_1_0_1; -.
DR   InParanoid; A6QLK6; -.
DR   OMA; GRCHGHV; -.
DR   OrthoDB; 1091250at2759; -.
DR   TreeFam; TF354288; -.
DR   Proteomes; UP000009136; Chromosome 19.
DR   Bgee; ENSBTAG00000003707; Expressed in nasopharynx and 100 other tissues.
DR   ExpressionAtlas; A6QLK6; baseline and differential.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0008180; C:COP9 signalosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0098793; C:presynapse; ISS:UniProtKB.
DR   GO; GO:0005154; F:epidermal growth factor receptor binding; IBA:GO_Central.
DR   GO; GO:0001784; F:phosphotyrosine residue binding; IBA:GO_Central.
DR   GO; GO:0043408; P:regulation of MAPK cascade; IBA:GO_Central.
DR   GO; GO:0007605; P:sensory perception of sound; ISS:UniProtKB.
DR   GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR   CDD; cd11948; SH3_GRAP_N; 1.
DR   Gene3D; 3.30.505.10; -; 1.
DR   InterPro; IPR035645; GRAP_N_SH3.
DR   InterPro; IPR043539; Grb2-like.
DR   InterPro; IPR000980; SH2.
DR   InterPro; IPR036860; SH2_dom_sf.
DR   InterPro; IPR036028; SH3-like_dom_sf.
DR   InterPro; IPR001452; SH3_domain.
DR   PANTHER; PTHR46037; PTHR46037; 1.
DR   Pfam; PF00017; SH2; 1.
DR   Pfam; PF00018; SH3_1; 2.
DR   PRINTS; PR00401; SH2DOMAIN.
DR   PRINTS; PR00452; SH3DOMAIN.
DR   SMART; SM00252; SH2; 1.
DR   SMART; SM00326; SH3; 2.
DR   SUPFAM; SSF50044; SSF50044; 2.
DR   SUPFAM; SSF55550; SSF55550; 1.
DR   PROSITE; PS50001; SH2; 1.
DR   PROSITE; PS50002; SH3; 2.
PE   2: Evidence at transcript level;
KW   Membrane; Reference proteome; Repeat; SH2 domain; SH3 domain; Synapse.
FT   CHAIN           1..217
FT                   /note="GRB2-related adapter protein"
FT                   /id="PRO_0000344237"
FT   DOMAIN          1..58
FT                   /note="SH3 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   DOMAIN          60..152
FT                   /note="SH2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT   DOMAIN          158..217
FT                   /note="SH3 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
SQ   SEQUENCE   217 AA;  25367 MW;  11EEB0D6F7122D41 CRC64;
     MESVALYSFQ ATESDELAFN KGDTLKILNM EDDQNWYKAE LRGAEGFVPK NYIRLKPHPW
     YSGRISRQLA EEILMKRNHQ GAFLIRESES SPGEFSVSVN YGDQVQHFKV LRDPSGKYYL
     WEEKFNSLNE LVAFYRTTTI AKKRQVFLQD EEPLPKPPRA CFAQAQFDFS AQDPSQLSFR
     RGDIIEVLER LDPSWWRGRL SGRIGFFPRS YVQPVHM