GRASP_HUMAN
ID GRASP_HUMAN Reviewed; 395 AA.
AC Q7Z6J2; Q6PIF8; Q7Z741;
DT 27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Protein TAMALIN {ECO:0000305};
DE AltName: Full=General receptor for phosphoinositides 1-associated scaffold protein {ECO:0000305};
DE Short=GRP1-associated scaffold protein;
GN Name=TAMALIN {ECO:0000312|HGNC:HGNC:18707}; Synonyms=GRASP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Ovary, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-237, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
CC -!- FUNCTION: Plays a role in intracellular trafficking and contributes to
CC the macromolecular organization of group 1 metabotropic glutamate
CC receptors (mGluRs) at synapses. {ECO:0000250}.
CC -!- SUBUNIT: Heteromer. Composed of TAMALIN, CYTH2 and at least one GRM1.
CC Also interacts with CYTH3, GRM2, GRM3 and GRM5 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:Q8R4T5}. Cell membrane
CC {ECO:0000250|UniProtKB:Q8R4T5}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q8R4T5}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:Q8R4T5}. Postsynaptic cell membrane
CC {ECO:0000250|UniProtKB:Q8R4T5}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7Z6J2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7Z6J2-2; Sequence=VSP_015707, VSP_015708;
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DR EMBL; BC035500; AAH35500.1; -; mRNA.
DR EMBL; BC052967; AAH52967.1; -; mRNA.
DR EMBL; BC053666; AAH53666.1; -; mRNA.
DR CCDS; CCDS61124.1; -. [Q7Z6J2-2]
DR CCDS; CCDS8817.1; -. [Q7Z6J2-1]
DR RefSeq; NP_001258785.1; NM_001271856.1. [Q7Z6J2-2]
DR RefSeq; NP_859062.1; NM_181711.3. [Q7Z6J2-1]
DR PDB; 2PNT; X-ray; 2.15 A; A/B=97-188.
DR PDBsum; 2PNT; -.
DR AlphaFoldDB; Q7Z6J2; -.
DR SMR; Q7Z6J2; -.
DR BioGRID; 127762; 12.
DR CORUM; Q7Z6J2; -.
DR IntAct; Q7Z6J2; 2.
DR STRING; 9606.ENSP00000293662; -.
DR iPTMnet; Q7Z6J2; -.
DR PhosphoSitePlus; Q7Z6J2; -.
DR BioMuta; GRASP; -.
DR DMDM; 74738818; -.
DR EPD; Q7Z6J2; -.
DR jPOST; Q7Z6J2; -.
DR MassIVE; Q7Z6J2; -.
DR PaxDb; Q7Z6J2; -.
DR PeptideAtlas; Q7Z6J2; -.
DR PRIDE; Q7Z6J2; -.
DR ProteomicsDB; 69419; -. [Q7Z6J2-1]
DR ProteomicsDB; 69420; -. [Q7Z6J2-2]
DR Antibodypedia; 26519; 95 antibodies from 23 providers.
DR DNASU; 160622; -.
DR Ensembl; ENST00000293662.9; ENSP00000293662.4; ENSG00000161835.11. [Q7Z6J2-1]
DR Ensembl; ENST00000552049.5; ENSP00000449492.1; ENSG00000161835.11. [Q7Z6J2-2]
DR GeneID; 160622; -.
DR KEGG; hsa:160622; -.
DR MANE-Select; ENST00000293662.9; ENSP00000293662.4; NM_181711.4; NP_859062.1.
DR UCSC; uc001rzo.3; human. [Q7Z6J2-1]
DR CTD; 160622; -.
DR DisGeNET; 160622; -.
DR GeneCards; TAMALIN; -.
DR HGNC; HGNC:18707; TAMALIN.
DR HPA; ENSG00000161835; Low tissue specificity.
DR MIM; 612027; gene.
DR neXtProt; NX_Q7Z6J2; -.
DR OpenTargets; ENSG00000161835; -.
DR VEuPathDB; HostDB:ENSG00000161835; -.
DR eggNOG; KOG3528; Eukaryota.
DR GeneTree; ENSGT00530000063734; -.
DR HOGENOM; CLU_058640_0_0_1; -.
DR InParanoid; Q7Z6J2; -.
DR OMA; YQTCIYQ; -.
DR OrthoDB; 966869at2759; -.
DR PhylomeDB; Q7Z6J2; -.
DR TreeFam; TF316315; -.
DR PathwayCommons; Q7Z6J2; -.
DR SignaLink; Q7Z6J2; -.
DR BioGRID-ORCS; 160622; 40 hits in 1067 CRISPR screens.
DR ChiTaRS; GRASP; human.
DR EvolutionaryTrace; Q7Z6J2; -.
DR GenomeRNAi; 160622; -.
DR Pharos; Q7Z6J2; Tbio.
DR PRO; PR:Q7Z6J2; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q7Z6J2; protein.
DR Bgee; ENSG00000161835; Expressed in right lung and 165 other tissues.
DR ExpressionAtlas; Q7Z6J2; baseline and differential.
DR Genevisible; Q7Z6J2; HS.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0098978; C:glutamatergic synapse; IEA:Ensembl.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0014069; C:postsynaptic density; IEA:Ensembl.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0098685; C:Schaffer collateral - CA1 synapse; IEA:Ensembl.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0030165; F:PDZ domain binding; IEA:Ensembl.
DR GO; GO:0031267; F:small GTPase binding; IEA:Ensembl.
DR GO; GO:0008104; P:protein localization; IEA:Ensembl.
DR GO; GO:0099152; P:regulation of neurotransmitter receptor transport, endosome to postsynaptic membrane; IEA:Ensembl.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR Gene3D; 2.30.42.10; -; 1.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR Pfam; PF00595; PDZ; 1.
DR SMART; SM00228; PDZ; 1.
DR SUPFAM; SSF50156; SSF50156; 1.
DR PROSITE; PS50106; PDZ; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Cytoplasm; Membrane;
KW Methylation; Phosphoprotein; Postsynaptic cell membrane;
KW Reference proteome; Synapse.
FT CHAIN 1..395
FT /note="Protein TAMALIN"
FT /id="PRO_0000087584"
FT DOMAIN 101..190
FT /note="PDZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 181..258
FT /note="Interaction with PSCD3"
FT /evidence="ECO:0000250"
FT REGION 293..349
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..48
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..319
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 77
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9JJA9"
FT MOD_RES 94
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8R4T5"
FT MOD_RES 237
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:19690332"
FT MOD_RES 270
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q9JJA9"
FT MOD_RES 387
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9JJA9"
FT VAR_SEQ 1..143
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_015707"
FT VAR_SEQ 144..151
FT /note="AQLAGLTP -> MTLLPSKG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_015708"
FT STRAND 98..105
FT /evidence="ECO:0007829|PDB:2PNT"
FT STRAND 114..121
FT /evidence="ECO:0007829|PDB:2PNT"
FT STRAND 130..138
FT /evidence="ECO:0007829|PDB:2PNT"
FT HELIX 143..146
FT /evidence="ECO:0007829|PDB:2PNT"
FT STRAND 154..158
FT /evidence="ECO:0007829|PDB:2PNT"
FT HELIX 168..177
FT /evidence="ECO:0007829|PDB:2PNT"
FT TURN 178..180
FT /evidence="ECO:0007829|PDB:2PNT"
FT STRAND 181..188
FT /evidence="ECO:0007829|PDB:2PNT"
SQ SEQUENCE 395 AA; 42623 MW; B042CA2298753CB9 CRC64;
MTLRRLRKLQ QKEEAAATPD PAARTPDSEV APAAPVPTPG PPAAAATPGP PADELYAALE
DYHPAELYRA LAVSGGTLPR RKGSGFRWKN LSQSPEQQRK VLTLEKEDNQ TFGFEIQTYG
LHHREEQRVE MVTFVCRVHE SSPAQLAGLT PGDTIASVNG LNVEGIRHRE IVDIIKASGN
VLRLETLYGT SIRKAELEAR LQYLKQTLYE KWGEYRSLMV QEQRLVHGLV VKDPSIYDTL
ESVRSCLYGA GLLPGSLPFG PLLAVPGRPR GGARRARGDA DDAVYHTCFF GDSEPPALPP
PPPPARAFGP GPAETPAVGP GPGPRAALSR SASVRCAGPG GGGGGGAPGA LWTEAREQAL
CGPGLRKTKY RSFRRRLLKF IPGLNRSLEE EESQL
