ID   GRAS_STAS1              Reviewed;         342 AA.
AC   Q49VK4;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 2.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Sensor histidine kinase GraS;
DE            EC=2.7.13.3;
DE   AltName: Full=Glycopeptide resistance-associated protein S;
GN   Name=graS; OrderedLocusNames=SSP2061;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- FUNCTION: Member of the two-component regulatory system GraR/GraS
CC       involved in resistance against cationic antimicrobial peptides (CAMPs).
CC       GraS probably functions as a sensor protein kinase which is
CC       autophosphorylated at a histidine residue and transfers its phosphate
CC       group to GraR (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- PTM: Autophosphorylated. {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE19206.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AP008934; BAE19206.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_041784838.1; NZ_MTGA01000039.1.
DR   AlphaFoldDB; Q49VK4; -.
DR   SMR; Q49VK4; -.
DR   STRING; 342451.SSP2061; -.
DR   EnsemblBacteria; BAE19206; BAE19206; SSP2061.
DR   KEGG; ssp:SSP2061; -.
DR   PATRIC; fig|342451.11.peg.2054; -.
DR   eggNOG; COG2205; Bacteria.
DR   HOGENOM; CLU_000445_13_1_9; -.
DR   OrthoDB; 1827824at2; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF02518; HATPase_c; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Cell membrane; Kinase; Membrane;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase;
KW   Transmembrane; Transmembrane helix; Two-component regulatory system;
KW   Virulence.
FT   CHAIN           1..342
FT                   /note="Sensor histidine kinase GraS"
FT                   /id="PRO_0000347930"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          126..332
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         129
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   342 AA;  40364 MW;  59C36C286B8B12B2 CRC64;
     MANLKWFFIF LRSRIYWILW LLFLHFIFLG IAYLDYDISV YSILYIIILN IGLSVLFLIF
     TYLKEVKFYK HLDENIEPEA LKHKSLADTP FQQEMVNYLY GQITNQKALV THQKQQIQST
     EAALTDFVHD IKTPVTAMKL LIEKEQDLSR KHALLYEWSR INDMLDRQLF LTRLEYQNKD
     MYFEHVPLKR LVIEEIQITR YISQSKGIGY DLDFEDTLNV YTDTKWCRMM IRQVLSNAVK
     YSEESMIHIR ALKESRHVVL EIKDEGKGIS EKDLPRIFDK GFTSTSNRNN TISSGLGLYL
     VNHVKEKLSI CVRIHSELDK GTVVKFIFPN QNEIVAKLSQ NI