GRB2A_XENLA
ID GRB2A_XENLA Reviewed; 229 AA.
AC P87379; Q6GME6; Q9PU11;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Growth factor receptor-bound protein 2-A;
DE AltName: Full=Adapter protein GRB2-A;
DE AltName: Full=SH2/SH3 adapter GRB2-A;
GN Name=grb2-a;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Lu W., Mayer B.J.;
RL Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Embryo;
RA Goisset C., Shi D.L., Boucaut J.-C.;
RT "Selective inhibition of neural induction but not mesoderm induction by
RT interfering mutants of Sem-5/Grb2.";
RL Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DOMAIN, AND MUTAGENESIS OF PRO-49 AND GLY-215.
RX PubMed=11257447; DOI=10.1016/s0898-6568(00)00138-8;
RA Cailliau K., Browaeys-Poly E., Broutin-L'Hermite I., Nioche P., Garbay C.,
RA Ducruix A., Vilain J.P.;
RT "Grb2 promotes reinitiation of meiosis in Xenopus oocytes.";
RL Cell. Signal. 13:51-55(2001).
CC -!- FUNCTION: Adapter protein that provides a critical link between cell
CC surface growth factor receptors and the Ras signaling pathway (By
CC similarity). Promotes meiotic reinitiation during oocyte maturation.
CC {ECO:0000250, ECO:0000269|PubMed:11257447}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Endosome {ECO:0000250}. Golgi apparatus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P87379-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P87379-2; Sequence=VSP_017084;
CC -!- DOMAIN: The two SH3 domains and the SH2 domain are necessary for the
CC reinitiation of oocyte meiosis. {ECO:0000269|PubMed:11257447}.
CC -!- MISCELLANEOUS: [Isoform 1]: May be due to partial intron retention.
CC -!- SIMILARITY: Belongs to the GRB2/sem-5/DRK family. {ECO:0000305}.
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DR EMBL; U89775; AAB49699.1; -; mRNA.
DR EMBL; AJ223061; CAB59279.1; -; mRNA.
DR EMBL; BC074118; AAH74118.1; -; mRNA.
DR RefSeq; NP_001084357.1; NM_001090888.1.
DR RefSeq; XP_018089703.1; XM_018234214.1. [P87379-1]
DR RefSeq; XP_018089704.1; XM_018234215.1. [P87379-1]
DR AlphaFoldDB; P87379; -.
DR BMRB; P87379; -.
DR SMR; P87379; -.
DR IntAct; P87379; 2.
DR MINT; P87379; -.
DR GeneID; 399458; -.
DR KEGG; xla:399458; -.
DR CTD; 399458; -.
DR Xenbase; XB-GENE-6253891; grb2.L.
DR OMA; DFSLSVX; -.
DR Proteomes; UP000186698; Chromosome 9_10L.
DR Bgee; 399458; Expressed in spleen and 19 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005154; F:epidermal growth factor receptor binding; ISS:UniProtKB.
DR GO; GO:0043560; F:insulin receptor substrate binding; ISS:UniProtKB.
DR GO; GO:0007143; P:female meiotic nuclear division; IDA:UniProtKB.
DR GO; GO:0008286; P:insulin receptor signaling pathway; ISS:UniProtKB.
DR CDD; cd11949; SH3_GRB2_C; 1.
DR CDD; cd11946; SH3_GRB2_N; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR035643; GRB2_C_SH3.
DR InterPro; IPR035641; GRB2_N_SH3.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR Pfam; PF00017; SH2; 1.
DR Pfam; PF00018; SH3_1; 2.
DR PRINTS; PR00401; SH2DOMAIN.
DR PRINTS; PR00452; SH3DOMAIN.
DR SMART; SM00252; SH2; 1.
DR SMART; SM00326; SH3; 2.
DR SUPFAM; SSF50044; SSF50044; 2.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50001; SH2; 1.
DR PROSITE; PS50002; SH3; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Endosome;
KW Golgi apparatus; Meiosis; Nucleus; Reference proteome; Repeat; SH2 domain;
KW SH3 domain.
FT CHAIN 1..229
FT /note="Growth factor receptor-bound protein 2-A"
FT /id="PRO_0000088203"
FT DOMAIN 1..58
FT /note="SH3 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT DOMAIN 60..152
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT DOMAIN 168..227
FT /note="SH3 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT VAR_SEQ 155..166
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1, ECO:0000303|Ref.2"
FT /id="VSP_017084"
FT MUTAGEN 49
FT /note="P->L: Unable to reinitiate oocyte meiosis."
FT /evidence="ECO:0000269|PubMed:11257447"
FT MUTAGEN 215
FT /note="G->R: Unable to reinitiate oocyte meiosis."
FT /evidence="ECO:0000269|PubMed:11257447"
FT CONFLICT 27
FT /note="V -> A (in Ref. 2; CAB59279)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="R -> P (in Ref. 1; AAB49699)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 229 AA; 26383 MW; 7B278E2DC6B33133 CRC64;
MEAIAKYDFK ATADDELSFK RGDVLKVLNE ECDQNWYKAE LNGKDGFIPK NYIEMKAHPW
FFGKIPRAKA EEMLGKQRHD GAFLIRESES APGDFSLSVK FGNDVQHFKV LRDGAGKYFL
WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD IEQVPQVHGG DRATSLPQQP TYVQALFDFD
PQEDGELGFR RGDFIQVVDN SDPNWWKGTC LSQTGMFPRN YVTPVNRNM
