ID   GRC3_ASHGO              Reviewed;         633 AA.
AC   Q753Y3;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Polynucleotide 5'-hydroxyl-kinase GRC3;
DE            EC=2.7.1.-;
GN   Name=GRC3; OrderedLocusNames=AFR189C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Polynucleotide 5'-kinase involved in rRNA processing.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Clp1 family. NOL9/GRC3 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE016819; AAS53560.1; -; Genomic_DNA.
DR   RefSeq; NP_985736.1; NM_211090.1.
DR   AlphaFoldDB; Q753Y3; -.
DR   SMR; Q753Y3; -.
DR   STRING; 33169.AAS53560; -.
DR   EnsemblFungi; AAS53560; AAS53560; AGOS_AFR189C.
DR   GeneID; 4621994; -.
DR   KEGG; ago:AGOS_AFR189C; -.
DR   eggNOG; KOG2750; Eukaryota.
DR   HOGENOM; CLU_010345_1_1_1; -.
DR   OMA; EHVWKVR; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0090730; C:Las1 complex; IEA:EnsemblFungi.
DR   GO; GO:0030874; C:nucleolar chromatin; IEA:EnsemblFungi.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0051731; F:polynucleotide 5'-hydroxyl-kinase activity; ISS:UniProtKB.
DR   GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IBA:GO_Central.
DR   GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR   GO; GO:0006363; P:termination of RNA polymerase I transcription; IEA:EnsemblFungi.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR045116; Clp1/Grc3.
DR   InterPro; IPR032319; CLP1_P.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12755; PTHR12755; 1.
DR   Pfam; PF16575; CLP1_P; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Nucleotide-binding; Nucleus; Reference proteome;
KW   rRNA processing; Transferase.
FT   CHAIN           1..633
FT                   /note="Polynucleotide 5'-hydroxyl-kinase GRC3"
FT                   /id="PRO_0000087587"
FT   BINDING         243..250
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   633 AA;  71137 MW;  3C36C5CB3BDAFE84 CRC64;
     MSDDFVVPTY EVGGSDSGSD QDISDIEEGS FNGISATPLP TADVEYVSEA SPMDATASLG
     YLSGAEGANL YVAREGANYF NDAANKAVVL CLHSYEQLAI SGVFKLQVMK GGITYNSIHY
     NCGLHTYDFW HPLSESIPPI AASFAADWEE HSYGLSPWLP ADIKEEYECI LRISEGSNIG
     DVGRLMAELG QLWTRHTGCT FSILRPEDYA RPLILNKDWD NLQAELMIHH MNSEHDMRVM
     CIGGKNSGKS TLLRLLLQKF LHGNKSTTKA TGVSHEADVV NYLDMDPGQP EYSAPDSISW
     SKLTSETLSL GQHLAQGHRE MIQTTYIGSS TPQTWPEMYL TAMEHIIHKW EMEGQMNTAL
     LNLPGWIKGF GITIINKAME LFKPTHIIFL THGGKLISKE LVVPNMFETM QKGKYKPMIY
     NLQAFTQHNI PGLPAQDPRY HAANIRSFRL LAHLHRLSEQ SYYPGPLLSS PPLQVSFGSQ
     GIMAFRFLQD PSSGYHQDDI SAALQGCVVC LYHSKMPPDI DMCGVFPMLK HSADYTSMDF
     VTLALIHSID VERKFMNIYI PGHVSGRVHS LKNNFVLVRG ATDLPLCELY PQKLLSSSYR
     NREIPYVSFS KRKKYEYVWK IRKNVRRRGH YNK