ID   GRC3_CRYNJ              Reviewed;         744 AA.
AC   P0CM78; Q55U98; Q5KI47;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Polynucleotide 5'-hydroxyl-kinase GRC3;
DE            EC=2.7.1.-;
GN   Name=GRC3; OrderedLocusNames=CND04240;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Polynucleotide 5'-kinase involved in rRNA processing.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Clp1 family. NOL9/GRC3 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE017344; AAW43285.1; -; Genomic_DNA.
DR   RefSeq; XP_570592.1; XM_570592.1.
DR   AlphaFoldDB; P0CM78; -.
DR   SMR; P0CM78; -.
DR   STRING; 5207.AAW43285; -.
DR   PaxDb; P0CM78; -.
DR   eggNOG; KOG2750; Eukaryota.
DR   HOGENOM; CLU_010345_0_0_1; -.
DR   InParanoid; P0CM78; -.
DR   OMA; PLYQRIW; -.
DR   OrthoDB; 1217334at2759; -.
DR   Proteomes; UP000002149; Chromosome 4.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0051731; F:polynucleotide 5'-hydroxyl-kinase activity; ISS:UniProtKB.
DR   GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IBA:GO_Central.
DR   GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR045116; Clp1/Grc3.
DR   InterPro; IPR032319; CLP1_P.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12755; PTHR12755; 1.
DR   Pfam; PF16575; CLP1_P; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Nucleotide-binding; Nucleus; Reference proteome;
KW   rRNA processing; Transferase.
FT   CHAIN           1..744
FT                   /note="Polynucleotide 5'-hydroxyl-kinase GRC3"
FT                   /id="PRO_0000087591"
FT   REGION          1..131
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        60..101
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         331..338
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   744 AA;  81009 MW;  777CE03E78095530 CRC64;
     MSALAARRAT AAAASPKPEQ PESSIEEVSV SGALTLPSPK RRKTRQTSPK PRSKARYSDD
     VPTSRQFFQA TESLAEQRTG RFSPSAPDSD GGTSSSSVGD SDEDMAQEDE FEDRREVDGE
     RDQRKVSVAA NMSSSGPFKS LDLMPVDITS QFNPKDNVNF CRITEGQLAS AEMNDGHPGP
     GVIVSLARNE SLTIAGLFLL TPLQNTLSIY STALSPSMSS FPVYAPTSHP LPVISPASTQ
     APGKESDKTL LLIRENRCGI DGLRNGAVPG FSNIWLEDNG PWGLRGVHPV VGSFPVPVYP
     YCTPPSWSHA ISSLSSSDVN LQTPFVGLVK GPKRSGKSTF ARALLNNLLR RFRKVAWLEC
     DLGQGEFGSG AVVGLWILDK PALGPPFTHP LLPSRSHYLG TYTPLTCPDE YLVAIRHLIE
     HYKYELQYTS EYSALHTTVH DKISTHVPLV INTQGWMKGL GEELLNVIES MAQPTRVFSF
     ESQSEEVYSG QGWTSTPPWQ ATQLPYDPAY PTTEPVETEV TQTYSLETAP VSALQARYTP
     ADLRVLSAIT YFHASLHPTQ SVPVTWDISS PLVCTIPWEV ELGIGKALEK VYLIGEGSEG
     VLEEDLPIAL NGAIVALAEM LGSYEDEPTV YEQGRSPPPT DLVNILGLAV IRSLSSGNSV
     NPGLKLQLLT PLPPSYLSRA RILIKSGALE LPLPGMIDWR RGGINEEGML GKGWEEIPFL
     DVGGLDVIGG ERRRFRKNIM RKGM