ID   GRC3_KLULA              Reviewed;         631 AA.
AC   Q6CQ06;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Polynucleotide 5'-hydroxyl-kinase GRC3;
DE            EC=2.7.1.-;
GN   Name=GRC3; OrderedLocusNames=KLLA0E00748g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Polynucleotide 5'-kinase involved in rRNA processing.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Clp1 family. NOL9/GRC3 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CR382125; CAG99070.1; -; Genomic_DNA.
DR   RefSeq; XP_453983.1; XM_453983.1.
DR   AlphaFoldDB; Q6CQ06; -.
DR   SMR; Q6CQ06; -.
DR   STRING; 28985.XP_453983.1; -.
DR   EnsemblFungi; CAG99070; CAG99070; KLLA0_E00815g.
DR   GeneID; 2894158; -.
DR   KEGG; kla:KLLA0_E00815g; -.
DR   eggNOG; KOG2750; Eukaryota.
DR   HOGENOM; CLU_010345_1_1_1; -.
DR   InParanoid; Q6CQ06; -.
DR   OMA; EHVWKVR; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0090730; C:Las1 complex; IEA:EnsemblFungi.
DR   GO; GO:0030874; C:nucleolar chromatin; IEA:EnsemblFungi.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0051731; F:polynucleotide 5'-hydroxyl-kinase activity; ISS:UniProtKB.
DR   GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR   GO; GO:0006363; P:termination of RNA polymerase I transcription; IEA:EnsemblFungi.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR045116; Clp1/Grc3.
DR   InterPro; IPR032319; CLP1_P.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12755; PTHR12755; 1.
DR   Pfam; PF16575; CLP1_P; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Nucleotide-binding; Nucleus; Reference proteome;
KW   rRNA processing; Transferase.
FT   CHAIN           1..631
FT                   /note="Polynucleotide 5'-hydroxyl-kinase GRC3"
FT                   /id="PRO_0000087594"
FT   REGION          1..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        15..30
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         254..261
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   631 AA;  72566 MW;  E6AD068E01996260 CRC64;
     MSEDILTYTA NTDSDDSDVE QREIEKKYKT SSETPVVQLY DGDDYDNDDI RETNGNDFPN
     EEDESNQLYS PVVDMNFFTL SDECTEVIIC LEKNQHLLIC GQFNLQIIKG GITYNNVHYN
     SGYKNWEFWH PACNAISPIV SSFYAGWESK LFLNREYQHF TTQIESYDCV LKISNGCNIT
     ELSSLLPELR DMWGPLNISL LPGIARKQLT FNIISKISDS VRILDISTEW STVIDEMRIF
     HANSSQDMRV MVIGGKNSGK STLMRLLVQK FLHSNNDLSY EAIHYLDIDP GQPEYSPPES
     ISWNKVDPKS MSLGQHLCQG RFTTIKQHFI GSSSPQDWPE TYSNAVESLL KEWQTENFMG
     TSFLNIPGWI KGFGVKIINH ALDHFKPTHI IFLSYNGLPF SSEIKVPDAF STTQRSTYKP
     IILQLHSPAV GKHISHALQR FNAAKIRQFK ITAYLHKKYD RNFVINPLVM QKPQLTSIGN
     RGIVGFEFLQ YSNSSLYHDD IKLSLDGTVV ALHRADSTPD VLFKGIFPVM KNYSKINTEF
     VGLALIHSID LEQSLIYMYI PENLFRALRE TENKWVLVRG KTETPICELY PNNGIFKSEV
     DIPYVSLYQK KRHEHVWKVR KNVMRRGHHM K