ID   GRCA_BPT4               Reviewed;         120 AA.
AC   P13316;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   02-DEC-2020, entry version 91.
DE   RecName: Full=Autonomous glycyl radical cofactor;
GN   Name=grcA; Synonyms=63.1, VS.6; ORFNames=Y06I;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3024113; DOI=10.1093/nar/14.21.8637;
RA   Valerie K., Stevens J., Lynch M., Henderson E.E., de Riel J.K.;
RT   "Nucleotide sequence and analysis of the 58.3 to 65.5-kb early region of
RT   bacteriophage T4.";
RL   Nucleic Acids Res. 14:8637-8654(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   FUNCTION.
RX   PubMed=11444864; DOI=10.1006/bbrc.2001.5186;
RA   Wagner A.F.V., Schultz S., Bomke J., Pils T., Lehmann W.D., Knappe J.;
RT   "YfiD of Escherichia coli and Y06I of bacteriophage T4 as autonomous glycyl
RT   radical cofactors reconstituting the catalytic center of oxygen-fragmented
RT   pyruvate formate-lyase.";
RL   Biochem. Biophys. Res. Commun. 285:456-462(2001).
CC   -!- FUNCTION: Acts as a radical domain for damaged PFL and possibly other
CC       radical proteins. {ECO:0000269|PubMed:11444864}.
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DR   EMBL; X04567; CAA28223.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42676.1; -; Genomic_DNA.
DR   RefSeq; NP_049730.1; NC_000866.4.
DR   SMR; P13316; -.
DR   GeneID; 1258600; -.
DR   KEGG; vg:1258600; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR   InterPro; IPR001150; Gly_radical.
DR   InterPro; IPR011140; Glycyl_radical_cofactor_GrcA.
DR   Pfam; PF01228; Gly_radical; 1.
DR   PIRSF; PIRSF000378; Gly_radicl_yfiD; 1.
DR   PROSITE; PS00850; GLY_RADICAL_1; 1.
DR   PROSITE; PS51149; GLY_RADICAL_2; 1.
PE   3: Inferred from homology;
KW   Organic radical; Reference proteome.
FT   CHAIN           1..120
FT                   /note="Autonomous glycyl radical cofactor"
FT                   /id="PRO_0000166717"
FT   DOMAIN          1..120
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00493"
FT   MOD_RES         95
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00493"
SQ   SEQUENCE   120 AA;  13814 MW;  263F1403363BC9A6 CRC64;
     MKAYQILEGT HKGTIYFEDG IQARIIVSKT FKEDSFVDPE IFYGLHAREI EIEPQPTVKI
     EGGQHLNVNV LRHETLEDAV KHPEKYPQLT IRVSGYAVRF NSLTPEQQRD VIARTFTESL