ID   GRCA_ECO5E              Reviewed;         127 AA.
AC   B5Z153;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Autonomous glycyl radical cofactor {ECO:0000255|HAMAP-Rule:MF_00806};
GN   Name=grcA {ECO:0000255|HAMAP-Rule:MF_00806};
GN   OrderedLocusNames=ECH74115_3816;
OS   Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=444450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC4115 / EHEC;
RX   PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA   Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC   -!- FUNCTION: Acts as a radical domain for damaged PFL and possibly other
CC       radical proteins. {ECO:0000255|HAMAP-Rule:MF_00806}.
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DR   EMBL; CP001164; ACI34993.1; -; Genomic_DNA.
DR   RefSeq; WP_000627807.1; NC_011353.1.
DR   AlphaFoldDB; B5Z153; -.
DR   SMR; B5Z153; -.
DR   GeneID; 66673531; -.
DR   KEGG; ecf:ECH74115_3816; -.
DR   HOGENOM; CLU_133780_0_0_6; -.
DR   OMA; LGQFEYR; -.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   HAMAP; MF_00806; GrcA; 1.
DR   InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR   InterPro; IPR001150; Gly_radical.
DR   InterPro; IPR011140; Glycyl_radical_cofactor_GrcA.
DR   Pfam; PF01228; Gly_radical; 1.
DR   PIRSF; PIRSF000378; Gly_radicl_yfiD; 1.
DR   TIGRFAMs; TIGR04365; spare_glycyl; 1.
DR   PROSITE; PS00850; GLY_RADICAL_1; 1.
DR   PROSITE; PS51149; GLY_RADICAL_2; 1.
PE   3: Inferred from homology;
KW   Acetylation; Organic radical.
FT   CHAIN           1..127
FT                   /note="Autonomous glycyl radical cofactor"
FT                   /id="PRO_1000133982"
FT   DOMAIN          5..127
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT   MOD_RES         48
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT   MOD_RES         88
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT   MOD_RES         92
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT   MOD_RES         102
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
SQ   SEQUENCE   127 AA;  14284 MW;  6BA617E67D6540CE CRC64;
     MITGIQITKA ANDDLLNSFW LLDSEKGEAR CIVAKAGYAE DEVVAVSKLG DIEYREVPVE
     VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV SGYAVRFNSL TPEQQRDVIA
     RTFTESL