GRCA_ECODH
ID GRCA_ECODH Reviewed; 127 AA.
AC B1XBQ6;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Autonomous glycyl radical cofactor {ECO:0000255|HAMAP-Rule:MF_00806};
GN Name=grcA {ECO:0000255|HAMAP-Rule:MF_00806};
GN OrderedLocusNames=ECDH10B_2747;
OS Escherichia coli (strain K12 / DH10B).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=316385;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / DH10B;
RX PubMed=18245285; DOI=10.1128/jb.01695-07;
RA Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B.,
RA Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B.,
RA Posfai G., Weinstock G.M., Blattner F.R.;
RT "The complete genome sequence of Escherichia coli DH10B: insights into the
RT biology of a laboratory workhorse.";
RL J. Bacteriol. 190:2597-2606(2008).
CC -!- FUNCTION: Acts as a radical domain for damaged PFL and possibly other
CC radical proteins. {ECO:0000255|HAMAP-Rule:MF_00806}.
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DR EMBL; CP000948; ACB03730.1; -; Genomic_DNA.
DR RefSeq; WP_000627807.1; NC_010473.1.
DR AlphaFoldDB; B1XBQ6; -.
DR SMR; B1XBQ6; -.
DR GeneID; 66673531; -.
DR KEGG; ecd:ECDH10B_2747; -.
DR HOGENOM; CLU_133780_0_0_6; -.
DR OMA; LGQFEYR; -.
DR BioCyc; ECOL316385:ECDH10B_RS13980-MON; -.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR HAMAP; MF_00806; GrcA; 1.
DR InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR InterPro; IPR001150; Gly_radical.
DR InterPro; IPR011140; Glycyl_radical_cofactor_GrcA.
DR Pfam; PF01228; Gly_radical; 1.
DR PIRSF; PIRSF000378; Gly_radicl_yfiD; 1.
DR TIGRFAMs; TIGR04365; spare_glycyl; 1.
DR PROSITE; PS00850; GLY_RADICAL_1; 1.
DR PROSITE; PS51149; GLY_RADICAL_2; 1.
PE 3: Inferred from homology;
KW Acetylation; Organic radical.
FT CHAIN 1..127
FT /note="Autonomous glycyl radical cofactor"
FT /id="PRO_1000133985"
FT DOMAIN 5..127
FT /note="Glycine radical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT MOD_RES 48
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT MOD_RES 88
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT MOD_RES 92
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT MOD_RES 102
FT /note="Glycine radical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
SQ SEQUENCE 127 AA; 14284 MW; 6BA617E67D6540CE CRC64;
MITGIQITKA ANDDLLNSFW LLDSEKGEAR CIVAKAGYAE DEVVAVSKLG DIEYREVPVE
VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV SGYAVRFNSL TPEQQRDVIA
RTFTESL