ID   GRCA_HAEIN              Reviewed;         127 AA.
AC   P44455;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Autonomous glycyl radical cofactor {ECO:0000255|HAMAP-Rule:MF_00806};
GN   Name=grcA {ECO:0000255|HAMAP-Rule:MF_00806}; OrderedLocusNames=HI_0017;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=9719565; DOI=10.1002/elps.1150191046;
RA   Fountoulakis M., Juranville J.-F., Roeder D., Evers S., Berndt P.,
RA   Langen H.;
RT   "Reference map of the low molecular mass proteins of Haemophilus
RT   influenzae.";
RL   Electrophoresis 19:1819-1827(1998).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=10675023;
RX   DOI=10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4;
RA   Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C.,
RA   Fountoulakis M.;
RT   "Two-dimensional map of the proteome of Haemophilus influenzae.";
RL   Electrophoresis 21:411-429(2000).
CC   -!- FUNCTION: Acts as a radical domain for damaged PFL and possibly other
CC       radical proteins. {ECO:0000255|HAMAP-Rule:MF_00806}.
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DR   EMBL; L42023; AAC21695.1; -; Genomic_DNA.
DR   PIR; C64140; C64140.
DR   RefSeq; NP_438190.1; NC_000907.1.
DR   RefSeq; WP_005628746.1; NC_000907.1.
DR   AlphaFoldDB; P44455; -.
DR   SMR; P44455; -.
DR   STRING; 71421.HI_0017; -.
DR   EnsemblBacteria; AAC21695; AAC21695; HI_0017.
DR   GeneID; 56957649; -.
DR   KEGG; hin:HI_0017; -.
DR   PATRIC; fig|71421.8.peg.17; -.
DR   eggNOG; COG3445; Bacteria.
DR   HOGENOM; CLU_133780_0_0_6; -.
DR   OMA; LGQFEYR; -.
DR   PhylomeDB; P44455; -.
DR   BioCyc; HINF71421:G1GJ1-17-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008861; F:formate C-acetyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006567; P:threonine catabolic process; IBA:GO_Central.
DR   HAMAP; MF_00806; GrcA; 1.
DR   InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR   InterPro; IPR001150; Gly_radical.
DR   InterPro; IPR011140; Glycyl_radical_cofactor_GrcA.
DR   Pfam; PF01228; Gly_radical; 1.
DR   PIRSF; PIRSF000378; Gly_radicl_yfiD; 1.
DR   TIGRFAMs; TIGR04365; spare_glycyl; 1.
DR   PROSITE; PS00850; GLY_RADICAL_1; 1.
DR   PROSITE; PS51149; GLY_RADICAL_2; 1.
PE   1: Evidence at protein level;
KW   Organic radical; Reference proteome.
FT   CHAIN           1..127
FT                   /note="Autonomous glycyl radical cofactor"
FT                   /id="PRO_0000166702"
FT   DOMAIN          5..127
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT   MOD_RES         102
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
SQ   SEQUENCE   127 AA;  14291 MW;  A3B896D3A479DB0A CRC64;
     MIKGIQITQA ANDNLLNSFW LLDSEKNEAR CLCAKGEFAE DQVVAVSELG QIEYRELPVN
     VAPTVKVEGG QHLNVNVLRR ETLEDAVNNP DKYPQLTIRV SGYAVRFNSL TPEQQRDVIT
     RTFTESL