ID   GRCA_SHIFL              Reviewed;         127 AA.
AC   Q83K21; Q7UBX2;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 2.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Autonomous glycyl radical cofactor {ECO:0000255|HAMAP-Rule:MF_00806};
GN   Name=grcA {ECO:0000255|HAMAP-Rule:MF_00806};
GN   OrderedLocusNames=SF2641, S2814;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Acts as a radical domain for damaged PFL and possibly other
CC       radical proteins. {ECO:0000255|HAMAP-Rule:MF_00806}.
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DR   EMBL; AE005674; AAN44137.2; -; Genomic_DNA.
DR   EMBL; AE014073; AAP17961.1; -; Genomic_DNA.
DR   RefSeq; NP_708430.2; NC_004337.2.
DR   RefSeq; WP_000627802.1; NZ_WPGW01000044.1.
DR   AlphaFoldDB; Q83K21; -.
DR   SMR; Q83K21; -.
DR   STRING; 198214.SF2641; -.
DR   EnsemblBacteria; AAN44137; AAN44137; SF2641.
DR   EnsemblBacteria; AAP17961; AAP17961; S2814.
DR   GeneID; 1026902; -.
DR   KEGG; sfl:SF2641; -.
DR   KEGG; sft:NCTC1_02903; -.
DR   KEGG; sfx:S2814; -.
DR   PATRIC; fig|198214.7.peg.3151; -.
DR   HOGENOM; CLU_133780_0_0_6; -.
DR   OMA; LGQFEYR; -.
DR   OrthoDB; 116406at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   HAMAP; MF_00806; GrcA; 1.
DR   InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR   InterPro; IPR001150; Gly_radical.
DR   InterPro; IPR011140; Glycyl_radical_cofactor_GrcA.
DR   Pfam; PF01228; Gly_radical; 1.
DR   PIRSF; PIRSF000378; Gly_radicl_yfiD; 1.
DR   TIGRFAMs; TIGR04365; spare_glycyl; 1.
DR   PROSITE; PS00850; GLY_RADICAL_1; 1.
DR   PROSITE; PS51149; GLY_RADICAL_2; 1.
PE   3: Inferred from homology;
KW   Acetylation; Organic radical; Reference proteome.
FT   CHAIN           1..127
FT                   /note="Autonomous glycyl radical cofactor"
FT                   /id="PRO_0000166710"
FT   DOMAIN          5..127
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT   MOD_RES         48
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT   MOD_RES         88
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT   MOD_RES         92
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
FT   MOD_RES         102
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00806"
SQ   SEQUENCE   127 AA;  14240 MW;  3EA6143D1608F615 CRC64;
     MITGIQITKA ANADLLNSFW LLDSEKGEAR CIVAKAGYAE DEVVAVSKLG DIEYREVPVE
     VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV SGYAVRFNSL TPEQQRDVIA
     RTFTESL