ID   GRDB_GOTPU              Reviewed;          35 AA.
AC   P55793;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Glycine reductase complex component B subunit gamma;
DE            EC=1.21.4.2;
DE   AltName: Full=Selenoprotein PB gamma;
DE   Flags: Fragment;
GN   Name=grdB;
OS   Gottschalkia purinilytica (Clostridium purinilyticum).
OC   Bacteria; Firmicutes; Tissierellia; Tissierellales; Gottschalkiaceae;
OC   Gottschalkia.
OX   NCBI_TaxID=1503;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33906 / DSM 1384 / WA1;
RX   PubMed=1825826; DOI=10.1128/jb.173.6.2093-2098.1991;
RA   Garcia G.E., Stadtman T.C.;
RT   "Selenoprotein A component of the glycine reductase complex from
RT   Clostridium purinolyticum: nucleotide sequence of the gene shows that
RT   selenocysteine is encoded by UGA.";
RL   J. Bacteriol. 173:2093-2098(1991).
CC   -!- FUNCTION: In the first step of glycine reductase, the substrate is
CC       bound to component PB via a Schiff base intermediate. Then the PB-
CC       activated substrate is nucleophilically attacked by the selenol anion
CC       of component PA to transform it to a carboxymethylated selenoether and
CC       the respective amine. By action of component PC, acetyl phosphate is
CC       formed, leaving component PA in its oxidized state. Finally component
CC       PA becomes reduced by the thioredoxin system to start a new catalytic
CC       cycle of reductive deamination.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + NH4(+) =
CC         [thioredoxin]-dithiol + glycine + H(+) + phosphate;
CC         Xref=Rhea:RHEA:12232, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:22191,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29950, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:50058, ChEBI:CHEBI:57305; EC=1.21.4.2;
CC   -!- SUBUNIT: Heterohexamer of two alpha, two beta and two gamma subunits.
CC       Component of the glycine reductase complex, together with components A
CC       and C. PB is substrate specific.
CC   -!- SIMILARITY: Belongs to the GrdB/GrdF/GrdH family. {ECO:0000305}.
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DR   EMBL; M64374; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P55793; -.
DR   BioCyc; MetaCyc:MON-13144; -.
DR   GO; GO:0030699; F:glycine reductase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR010187; Various_sel_PB.
DR   Pfam; PF07355; GRDB; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Selenocysteine.
FT   CHAIN           1..>35
FT                   /note="Glycine reductase complex component B subunit gamma"
FT                   /id="PRO_0000087599"
FT   NON_TER         35
SQ   SEQUENCE   35 AA;  3839 MW;  202A6F4B6A1D0675 CRC64;
     MIRVVHYIKE FFANIGGEEM AHVAPEVREG VVGPG