GRE1_ECOLI
ID GRE1_ECOLI Reviewed; 765 AA.
AC P32674; Q2M8P6;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Probable dehydratase PflD {ECO:0000305};
DE EC=4.2.1.- {ECO:0000305};
GN Name=pflD; Synonyms=yijL; OrderedLocusNames=b3951, JW3923;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP DISCUSSION OF SEQUENCE.
RX PubMed=7773398; DOI=10.1099/13500872-141-4-961;
RA Reizer J., Reizer A., Saier M.H. Jr.;
RT "Novel phosphotransferase system genes revealed by bacterial genome
RT analysis -- a gene cluster encoding a unique Enzyme I and the proteins of a
RT fructose-like permease system.";
RL Microbiology 141:961-971(1995).
RN [5]
RP PREDICTED FUNCTION.
RX PubMed=28183913; DOI=10.1126/science.aai8386;
RA Levin B.J., Huang Y.Y., Peck S.C., Wei Y., Martinez-Del Campo A.,
RA Marks J.A., Franzosa E.A., Huttenhower C., Balskus E.P.;
RT "A prominent glycyl radical enzyme in human gut microbiomes metabolizes
RT trans-4-hydroxy-L-proline.";
RL Science 355:1-28(2017).
CC -!- FUNCTION: Probably shows dehydratase activity.
CC {ECO:0000305|PubMed:28183913}.
CC -!- MISCELLANEOUS: Contains a dehydratase motif that is predictive of
CC enzyme function. {ECO:0000305|PubMed:28183913}.
CC -!- SIMILARITY: Belongs to the glycyl radical enzyme (GRE) family.
CC {ECO:0000305}.
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DR EMBL; U00006; AAC43057.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76933.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77360.1; -; Genomic_DNA.
DR PIR; B65202; B65202.
DR RefSeq; NP_418386.1; NC_000913.3.
DR RefSeq; WP_000184811.1; NZ_SSZK01000014.1.
DR AlphaFoldDB; P32674; -.
DR SMR; P32674; -.
DR BioGRID; 4262065; 3.
DR DIP; DIP-10469N; -.
DR IntAct; P32674; 4.
DR STRING; 511145.b3951; -.
DR PaxDb; P32674; -.
DR PRIDE; P32674; -.
DR EnsemblBacteria; AAC76933; AAC76933; b3951.
DR EnsemblBacteria; BAE77360; BAE77360; BAE77360.
DR GeneID; 948454; -.
DR KEGG; ecj:JW3923; -.
DR KEGG; eco:b3951; -.
DR PATRIC; fig|1411691.4.peg.2754; -.
DR EchoBASE; EB1854; -.
DR eggNOG; COG1882; Bacteria.
DR HOGENOM; CLU_009096_0_1_6; -.
DR InParanoid; P32674; -.
DR OMA; KGWGQPA; -.
DR PhylomeDB; P32674; -.
DR BioCyc; EcoCyc:EG11910-MON; -.
DR PRO; PR:P32674; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR InterPro; IPR001150; Gly_radical.
DR InterPro; IPR010098; PFL2/GDeHydtase_fam.
DR InterPro; IPR004184; PFL_dom.
DR Pfam; PF01228; Gly_radical; 1.
DR Pfam; PF02901; PFL-like; 1.
DR TIGRFAMs; TIGR01774; PFL2-3; 1.
DR PROSITE; PS00850; GLY_RADICAL_1; 1.
DR PROSITE; PS51149; GLY_RADICAL_2; 1.
DR PROSITE; PS51554; PFL; 1.
PE 3: Inferred from homology;
KW Lyase; Organic radical; Reference proteome.
FT CHAIN 1..765
FT /note="Probable dehydratase PflD"
FT /id="PRO_0000166689"
FT DOMAIN 3..637
FT /note="PFL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00887"
FT DOMAIN 645..765
FT /note="Glycine radical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00493"
FT MOD_RES 741
FT /note="Glycine radical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00493"
SQ SEQUENCE 765 AA; 85960 MW; 369D6B6E1BBC5BA0 CRC64;
MTNRISRLKT ALFANTREIS LERALLYTAS HRQTEGEPVI LRRAKATAYI LEHVEISIRD
EELIAGNRTV KPRAGIMSPE MDPYWLLKEL DQFPTRPQDR FAISEEDKRI YREELFPYWE
KRSMKDFING QMTDEVKAAT NTQIFSINQT DKGQGHIIID YPRLLNHGLG ELVAQMQQHC
QQQPENHFYQ AALLLLEASQ KHILRYAELA ETMAANCTDA QRREELLTIA EISRHNAQHK
PQTFWQACQL FWYMNIILQY ESNASSLSLG RFDQYMLPFY QTSLTQGEDA AFLKELLESL
WVKCNDIVLL RSTSSARYFA GFPTGYTALL GGLTENGRSA VNVLSFLCLD AYQSVQLPQP
NLGVRTNALI DTPFLMKTAE TIRFGTGIPQ IFNDEVVVPA FLNRGVSLED ARDYSVVGCV
ELSIPGRTYG LHDIAMFNLL KVMEICLHEN EGNAALTYEG LLEQIRAKIS HYITLMVEGS
NICDIGHRDW APVPLLSSFI SDCLEKGRDI TDGGARYNFS GVQGIGIANL SDSLHALKGM
VFEQQRLSFD ELLSVLKANF ATPEGEKVRA RLINRFEKYG NDIDEVDNIS AELLRHYCKE
VEKYQNPRGG YFTPGSYTVS AHVPLGSVVG ATPDGRFAGE QLADGGLSPM LGQDAQGPTA
VLKSVSKLDN TLLSNGTLLN VKFTPATLEG EAGLRKLADF LRAFTQLKLQ HIQFNVVNAD
TLREAQQRPQ DYAGLVVRVA GYSAFFVELS KEIQDDIIRR TAHQL
