GRE2_ECOLI
ID GRE2_ECOLI Reviewed; 810 AA.
AC P75793;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Probable dehydratase YbiW {ECO:0000305};
DE EC=4.2.1.- {ECO:0000305};
GN Name=ybiW; OrderedLocusNames=b0823, JW0807;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PREDICTED FUNCTION.
RX PubMed=28183913; DOI=10.1126/science.aai8386;
RA Levin B.J., Huang Y.Y., Peck S.C., Wei Y., Martinez-Del Campo A.,
RA Marks J.A., Franzosa E.A., Huttenhower C., Balskus E.P.;
RT "A prominent glycyl radical enzyme in human gut microbiomes metabolizes
RT trans-4-hydroxy-L-proline.";
RL Science 355:1-28(2017).
CC -!- FUNCTION: Probably shows dehydratase activity.
CC {ECO:0000305|PubMed:28183913}.
CC -!- MISCELLANEOUS: Contains a dehydratase motif that is predictive of
CC enzyme function. {ECO:0000305|PubMed:28183913}.
CC -!- SIMILARITY: Belongs to the glycyl radical enzyme (GRE) family.
CC {ECO:0000305}.
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DR EMBL; U00096; AAC73910.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35504.1; -; Genomic_DNA.
DR PIR; G64819; G64819.
DR RefSeq; NP_415344.1; NC_000913.3.
DR RefSeq; WP_000209309.1; NZ_LN832404.1.
DR AlphaFoldDB; P75793; -.
DR SMR; P75793; -.
DR BioGRID; 4259978; 3.
DR IntAct; P75793; 6.
DR STRING; 511145.b0823; -.
DR PaxDb; P75793; -.
DR PRIDE; P75793; -.
DR EnsemblBacteria; AAC73910; AAC73910; b0823.
DR EnsemblBacteria; BAA35504; BAA35504; BAA35504.
DR GeneID; 945444; -.
DR KEGG; ecj:JW0807; -.
DR KEGG; eco:b0823; -.
DR PATRIC; fig|1411691.4.peg.1455; -.
DR EchoBASE; EB3112; -.
DR eggNOG; COG1882; Bacteria.
DR InParanoid; P75793; -.
DR OMA; HVQYNIV; -.
DR PhylomeDB; P75793; -.
DR BioCyc; EcoCyc:G6426-MON; -.
DR PRO; PR:P75793; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR InterPro; IPR001150; Gly_radical.
DR InterPro; IPR010098; PFL2/GDeHydtase_fam.
DR InterPro; IPR004184; PFL_dom.
DR Pfam; PF01228; Gly_radical; 1.
DR Pfam; PF02901; PFL-like; 1.
DR TIGRFAMs; TIGR01774; PFL2-3; 1.
DR PROSITE; PS00850; GLY_RADICAL_1; 1.
DR PROSITE; PS51149; GLY_RADICAL_2; 1.
DR PROSITE; PS51554; PFL; 1.
PE 3: Inferred from homology;
KW Lyase; Organic radical; Reference proteome.
FT CHAIN 1..810
FT /note="Probable dehydratase YbiW"
FT /id="PRO_0000166690"
FT DOMAIN 11..682
FT /note="PFL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00887"
FT DOMAIN 689..810
FT /note="Glycine radical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00493"
FT REGION 677..699
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 786
FT /note="Glycine radical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00493"
SQ SEQUENCE 810 AA; 90125 MW; 8578C59C67F95DE1 CRC64;
MTTLKLDTLS DRIKAHKNAL VHIVKPPVCT ERAQHYTEMY QQHLDKPIPV RRALALAHHL
ANRTIWIKHD ELIIGNQASE VRAAPIFPEY TVSWIEKEID DLADRPGAGF AVSEENKRVL
HEVCPWWRGQ TVQDRCYGMF TDEQKGLLAT GIIKAEGNMT SGDAHLAVNF PLLLEKGLDG
LREEVAERRS RINLTVLEDL HGEQFLKAID IVLVAVSEHI ERFAALAREM AATETRESRR
DELLAMAENC DLIAHQPPQT FWQALQLCYF IQLILQIESN GHSVSFGRMD QYLYPYYRRD
VELNQTLDRE HAIEMLHSCW LKLLEVNKIR SGSHSKASAG SPLYQNVTIG GQNLVDGQPM
DAVNPLSYAI LESCGRLRST QPNLSVRYHA GMSNDFLDAC VQVIRCGFGM PAFNNDEIVI
PEFIKLGIEP QDAYDYAAIG CIETAVGGKW GYRCTGMSFI NFARVMLAAL EGGHDATSGK
VFLPQEKALS AGNFNNFDEV MDAWDTQIRY YTRKSIEIEY VVDTMLEENV HDILCSALVD
DCIERAKSIK QGGAKYDWVS GLQVGIANLG NSLAAVKKLV FEQGAIGQQQ LAAALADDFD
GLTHEQLRQR LINGAPKYGN DDDTVDTLLA RAYQTYIDEL KQYHNPRYGR GPVGGNYYAG
TSSISANVPF GAQTMATPDG RKAHTPLAEG ASPASGTDHL GPTAVIGSVG KLPTAAILGG
VLLNQKLNPA TLENESDKQK LMILLRTFFE VHKGWHIQYN IVSRETLLDA KKHPDQYRDL
VVRVAGYSAF FTALSPDAQD DIIARTEHML
