ID   GREA_BORBU              Reviewed;         901 AA.
AC   O51157;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 132.
DE   RecName: Full=Transcription elongation factor GreA;
DE   AltName: Full=Transcript cleavage factor GreA;
GN   Name=greA; OrderedLocusNames=BB_0132;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
CC   -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC       elongation past template-encoded arresting sites. The arresting sites
CC       in DNA have the property of trapping a certain fraction of elongating
CC       RNA polymerases that pass through, resulting in locked ternary
CC       complexes. Cleavage of the nascent transcript by cleavage factors such
CC       as GreA or GreB allows the resumption of elongation from the new
CC       3'terminus. GreA releases sequences of 2 to 3 nucleotides (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GreA/GreB family. {ECO:0000305}.
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DR   EMBL; AE000783; AAC66506.1; -; Genomic_DNA.
DR   PIR; D70116; D70116.
DR   RefSeq; NP_212266.1; NC_001318.1.
DR   RefSeq; WP_002665455.1; NC_001318.1.
DR   AlphaFoldDB; O51157; -.
DR   STRING; 224326.BB_0132; -.
DR   PRIDE; O51157; -.
DR   EnsemblBacteria; AAC66506; AAC66506; BB_0132.
DR   KEGG; bbu:BB_0132; -.
DR   PATRIC; fig|224326.49.peg.530; -.
DR   HOGENOM; CLU_322611_0_0_12; -.
DR   OMA; KKALHRY; -.
DR   Proteomes; UP000001807; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.287.180; -; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   Gene3D; 3.10.50.30; -; 1.
DR   HAMAP; MF_00105; GreA_GreB; 1.
DR   InterPro; IPR036953; GreA/GreB_C_sf.
DR   InterPro; IPR018151; TF_GreA/GreB_CS.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR   InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR   InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR   InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR   InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR   InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR   PANTHER; PTHR30437; PTHR30437; 1.
DR   Pfam; PF01272; GreA_GreB; 1.
DR   Pfam; PF03449; GreA_GreB_N; 1.
DR   SUPFAM; SSF46557; SSF46557; 1.
DR   TIGRFAMs; TIGR01462; greA; 1.
DR   PROSITE; PS00829; GREAB_1; 1.
DR   PROSITE; PS00830; GREAB_2; 1.
PE   3: Inferred from homology;
KW   Coiled coil; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..901
FT                   /note="Transcription elongation factor GreA"
FT                   /id="PRO_0000177006"
FT   DOMAIN          20..170
FT                   /note="GRAD2"
FT   DOMAIN          171..681
FT                   /note="GRAD1"
FT   REGION          741..901
FT                   /note="GREA"
FT   COILED          784..815
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   901 AA;  105315 MW;  FBB81088D735A5F9 CRC64;
     MSNVTTIEKL DSILQEDKWT RIVVNNYSLA KIKELDDLIN NIIDEGLTED VLDICGRHLK
     DVKKSIAGLY ISGMLIYSRR PLNDMNLLAV IDLFSQNLKW ALVEHICNEM LLISENKHAL
     YTLAKIYAQN NENDKLPGIW TRIVEADIDD TVFVRQLATY YETIDLQKSI FYFRKAIYRF
     IDKKQMSGIR EVWAKLIHYV SDDFDSFLLI LQKIEKDLGF KKAIVLYEDL FDHYSLTDNV
     DETIEILKGI LKLDNKNHKA RENLVKFLRE RYKDVKNIED YLVKSDIENL DKNFVDVYLD
     FEKYLFFAKG NFVYHQTWSV GIVRDVNDNG ITVDFVSKRG HFIGFDMAMS ALSPLSREDI
     RVLKAVTPKE ELADRVKKDI EWAIKVIIKS YKAIDLKGIK KELVPSLMTQ SSWNSWSLKA
     KQILKDNPHF VMDSGKLDCY IYNERSSNLN EKMYDKFKIE KDFYKRYEIF INYCNVKGIV
     KDLHIEEEML NYFLIYVNNF TKVDHHVVSS YVILKSLGNF SEELSSKVNI EKDVNLESLL
     KEYPKGIVDL FDSILNAEIK KELVVLIKEE LSNWILYYKE LFPHSVNKRL VESLYKEDPR
     EVEKLFNYVI KNYKIYKDAY IWILKHCKAY SIELSYSDSD LLSNLIKILT DSVIKINNKN
     NSVASKRIYK MVINLLVKDG YLKSVLEVVR DEELAKRVYM TCFYVKDFPP KDLLEIKSII
     RQLFDSVEFE DEKMQLAGER MEIGFLTILS SLNKKQKELK YLKEVEIPEN SKEIGKAREL
     GDLKENAEYH SAKEKQQFLT KRLNSLMLEI ENAKVIDTKD LQSSVVGFGT KVVILNEVTG
     KDESYLILGP WESNPDEGII SYKSPFGENL LDSREGDSLN FVINNTNFKY FVKKIEPIKF
     S