ID   GREA_CLOK1              Reviewed;         159 AA.
AC   B9DY72;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Transcription elongation factor GreA {ECO:0000255|HAMAP-Rule:MF_00105};
DE   AltName: Full=Transcript cleavage factor GreA {ECO:0000255|HAMAP-Rule:MF_00105};
GN   Name=greA {ECO:0000255|HAMAP-Rule:MF_00105}; OrderedLocusNames=CKR_0146;
OS   Clostridium kluyveri (strain NBRC 12016).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=583346;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 12016;
RA   Inui M., Nonaka H., Shinoda Y., Ikenaga Y., Abe M., Naito K., Vertes A.A.,
RA   Yukawa H.;
RT   "Complete genome sequence of Clostridium kluyveri and comparative genomics
RT   of Clostridia species.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC       elongation past template-encoded arresting sites. The arresting sites
CC       in DNA have the property of trapping a certain fraction of elongating
CC       RNA polymerases that pass through, resulting in locked ternary
CC       complexes. Cleavage of the nascent transcript by cleavage factors such
CC       as GreA or GreB allows the resumption of elongation from the new
CC       3'terminus. GreA releases sequences of 2 to 3 nucleotides.
CC       {ECO:0000255|HAMAP-Rule:MF_00105}.
CC   -!- SIMILARITY: Belongs to the GreA/GreB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00105}.
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DR   EMBL; AP009049; BAH05197.1; -; Genomic_DNA.
DR   RefSeq; WP_011988767.1; NC_011837.1.
DR   AlphaFoldDB; B9DY72; -.
DR   SMR; B9DY72; -.
DR   EnsemblBacteria; BAH05197; BAH05197; CKR_0146.
DR   KEGG; ckr:CKR_0146; -.
DR   HOGENOM; CLU_101379_2_1_9; -.
DR   Proteomes; UP000007969; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.287.180; -; 1.
DR   Gene3D; 3.10.50.30; -; 1.
DR   HAMAP; MF_00105; GreA_GreB; 1.
DR   InterPro; IPR036953; GreA/GreB_C_sf.
DR   InterPro; IPR018151; TF_GreA/GreB_CS.
DR   InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR   InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR   InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR   InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR   InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR   InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR   PANTHER; PTHR30437; PTHR30437; 1.
DR   Pfam; PF01272; GreA_GreB; 1.
DR   Pfam; PF03449; GreA_GreB_N; 1.
DR   PIRSF; PIRSF006092; GreA_GreB; 1.
DR   SUPFAM; SSF46557; SSF46557; 1.
DR   TIGRFAMs; TIGR01462; greA; 1.
DR   PROSITE; PS00829; GREAB_1; 1.
DR   PROSITE; PS00830; GREAB_2; 1.
PE   3: Inferred from homology;
KW   Coiled coil; DNA-binding; Transcription; Transcription regulation.
FT   CHAIN           1..159
FT                   /note="Transcription elongation factor GreA"
FT                   /id="PRO_1000118957"
FT   COILED          14..76
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00105"
SQ   SEQUENCE   159 AA;  17768 MW;  E2189A403A91F134 CRC64;
     MSGLKKYVMT YEGIKKLENE LEYLKTVKRK EITEKIKVAL SFGDLSENSE YDSAKNEQAF
     VEGRIVQLEN MLKNASMVDE DEVPLDIVGI GSIVKVKDYD LDEEVEYLIV GSAEADPINN
     KISNESPVGK GLVGKKPGDV IEIQVPDGVS KYKILNIRR