GREA_ECOLI
ID GREA_ECOLI Reviewed; 158 AA.
AC P0A6W5; P21346; P78111; Q2M931; Q8X9K9;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Transcription elongation factor GreA;
DE AltName: Full=Transcript cleavage factor GreA;
GN Name=greA; OrderedLocusNames=b3181, JW3148;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2243801; DOI=10.1093/nar/18.21.6443;
RA Sparkowski J., Das A.;
RT "The nucleotide sequence of greA, a suppressor gene that restores growth of
RT an Escherichia coli RNA polymerase mutant at high temperature.";
RL Nucleic Acids Res. 18:6443-6443(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Wang R., Kushner S.R.;
RL Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX PubMed=7854424; DOI=10.1038/373636a0;
RA Stebbins C.E., Borukhov S., Orlova M., Polyakov A., Goldfarb A.,
RA Darst S.A.;
RT "Crystal structure of the GreA transcript cleavage factor from Escherichia
RT coli.";
RL Nature 373:636-640(1995).
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreA releases sequences of 2 to 3 nucleotides.
CC -!- SIMILARITY: Belongs to the GreA/GreB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA57982.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X54718; CAA38521.1; -; Genomic_DNA.
DR EMBL; U01376; AAA97506.1; -; Genomic_DNA.
DR EMBL; U18997; AAA57982.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76213.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77225.1; -; Genomic_DNA.
DR PIR; G65108; G65108.
DR RefSeq; NP_417648.4; NC_000913.3.
DR RefSeq; WP_001148001.1; NZ_STEB01000012.1.
DR PDB; 1GRJ; X-ray; 2.20 A; A=1-158.
DR PDBsum; 1GRJ; -.
DR AlphaFoldDB; P0A6W5; -.
DR SMR; P0A6W5; -.
DR BioGRID; 4262064; 36.
DR DIP; DIP-35800N; -.
DR IntAct; P0A6W5; 52.
DR STRING; 511145.b3181; -.
DR SWISS-2DPAGE; P0A6W5; -.
DR jPOST; P0A6W5; -.
DR PaxDb; P0A6W5; -.
DR PRIDE; P0A6W5; -.
DR EnsemblBacteria; AAC76213; AAC76213; b3181.
DR EnsemblBacteria; BAE77225; BAE77225; BAE77225.
DR GeneID; 67415985; -.
DR GeneID; 947696; -.
DR KEGG; ecj:JW3148; -.
DR KEGG; eco:b3181; -.
DR PATRIC; fig|1411691.4.peg.3551; -.
DR EchoBASE; EB0410; -.
DR eggNOG; COG0782; Bacteria.
DR HOGENOM; CLU_101379_2_0_6; -.
DR InParanoid; P0A6W5; -.
DR OMA; TWLTQEA; -.
DR PhylomeDB; P0A6W5; -.
DR BioCyc; EcoCyc:EG10415-MON; -.
DR EvolutionaryTrace; P0A6W5; -.
DR PRO; PR:P0A6W5; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; HDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0001108; F:bacterial-type RNA polymerase holo enzyme binding; IDA:EcoCyc.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0006354; P:DNA-templated transcription, elongation; IDA:EcoCyc.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IDA:EcoCyc.
DR Gene3D; 1.10.287.180; -; 1.
DR Gene3D; 3.10.50.30; -; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR PANTHER; PTHR30437; PTHR30437; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR PIRSF; PIRSF006092; GreA_GreB; 1.
DR SUPFAM; SSF46557; SSF46557; 1.
DR TIGRFAMs; TIGR01462; greA; 1.
DR PROSITE; PS00829; GREAB_1; 1.
DR PROSITE; PS00830; GREAB_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..158
FT /note="Transcription elongation factor GreA"
FT /id="PRO_0000176921"
FT STRAND 4..7
FT /evidence="ECO:0007829|PDB:1GRJ"
FT HELIX 8..23
FT /evidence="ECO:0007829|PDB:1GRJ"
FT HELIX 25..37
FT /evidence="ECO:0007829|PDB:1GRJ"
FT HELIX 42..44
FT /evidence="ECO:0007829|PDB:1GRJ"
FT HELIX 46..71
FT /evidence="ECO:0007829|PDB:1GRJ"
FT STRAND 72..75
FT /evidence="ECO:0007829|PDB:1GRJ"
FT HELIX 77..79
FT /evidence="ECO:0007829|PDB:1GRJ"
FT STRAND 91..96
FT /evidence="ECO:0007829|PDB:1GRJ"
FT TURN 97..100
FT /evidence="ECO:0007829|PDB:1GRJ"
FT STRAND 101..108
FT /evidence="ECO:0007829|PDB:1GRJ"
FT TURN 110..112
FT /evidence="ECO:0007829|PDB:1GRJ"
FT HELIX 115..117
FT /evidence="ECO:0007829|PDB:1GRJ"
FT STRAND 119..124
FT /evidence="ECO:0007829|PDB:1GRJ"
FT HELIX 125..130
FT /evidence="ECO:0007829|PDB:1GRJ"
FT STRAND 150..156
FT /evidence="ECO:0007829|PDB:1GRJ"
SQ SEQUENCE 158 AA; 17641 MW; 9E7DFAFE838AC340 CRC64;
MQAIPMTLRG AEKLREELDF LKSVRRPEII AAIAEAREHG DLKENAEYHA AREQQGFCEG
RIKDIEAKLS NAQVIDVTKM PNNGRVIFGA TVTVLNLDSD EEQTYRIVGD DEADFKQNLI
SVNSPIARGL IGKEEDDVVV IKTPGGEVEF EVIKVEYL
