ID   GREA_RICPR              Reviewed;         162 AA.
AC   P27640;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Transcription elongation factor GreA {ECO:0000255|HAMAP-Rule:MF_00105};
DE   AltName: Full=Transcript cleavage factor GreA {ECO:0000255|HAMAP-Rule:MF_00105};
GN   Name=greA {ECO:0000255|HAMAP-Rule:MF_00105}; OrderedLocusNames=RP861;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=1641348; DOI=10.1093/nar/20.14.3785;
RA   Marks G.L., Wood D.O.;
RT   "Nucleotide sequence of the Rickettsia prowazekii greA homolog.";
RL   Nucleic Acids Res. 20:3785-3785(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9335295; DOI=10.1128/jb.179.20.6448-6452.1997;
RA   Shaw E.I., Marks G.L., Winkler H.H., Wood D.O.;
RT   "Transcriptional characterization of the Rickettsia prowazekii major
RT   macromolecular synthesis operon.";
RL   J. Bacteriol. 179:6448-6452(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
CC   -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC       elongation past template-encoded arresting sites. The arresting sites
CC       in DNA have the property of trapping a certain fraction of elongating
CC       RNA polymerases that pass through, resulting in locked ternary
CC       complexes. Cleavage of the nascent transcript by cleavage factors such
CC       as GreA or GreB allows the resumption of elongation from the new
CC       3'terminus. GreA releases sequences of 2 to 3 nucleotides.
CC       {ECO:0000255|HAMAP-Rule:MF_00105}.
CC   -!- SIMILARITY: Belongs to the GreA/GreB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00105}.
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DR   EMBL; Z12122; CAA78107.1; -; Genomic_DNA.
DR   EMBL; U02878; AAB81401.1; -; Unassigned_DNA.
DR   EMBL; AJ235273; CAA15285.1; -; Genomic_DNA.
DR   PIR; S26176; S26176.
DR   RefSeq; NP_221209.1; NC_000963.1.
DR   RefSeq; WP_004596757.1; NC_000963.1.
DR   AlphaFoldDB; P27640; -.
DR   SMR; P27640; -.
DR   STRING; 272947.RP861; -.
DR   EnsemblBacteria; CAA15285; CAA15285; CAA15285.
DR   GeneID; 57569984; -.
DR   KEGG; rpr:RP861; -.
DR   PATRIC; fig|272947.5.peg.900; -.
DR   eggNOG; COG0782; Bacteria.
DR   HOGENOM; CLU_101379_2_0_5; -.
DR   OMA; TWLTQEA; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.287.180; -; 1.
DR   Gene3D; 3.10.50.30; -; 1.
DR   HAMAP; MF_00105; GreA_GreB; 1.
DR   InterPro; IPR036953; GreA/GreB_C_sf.
DR   InterPro; IPR018151; TF_GreA/GreB_CS.
DR   InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR   InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR   InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR   InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR   InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR   InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR   PANTHER; PTHR30437; PTHR30437; 1.
DR   Pfam; PF01272; GreA_GreB; 1.
DR   Pfam; PF03449; GreA_GreB_N; 1.
DR   PIRSF; PIRSF006092; GreA_GreB; 1.
DR   SUPFAM; SSF46557; SSF46557; 1.
DR   TIGRFAMs; TIGR01462; greA; 1.
DR   PROSITE; PS00829; GREAB_1; 1.
DR   PROSITE; PS00830; GREAB_2; 1.
PE   3: Inferred from homology;
KW   Coiled coil; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..162
FT                   /note="Transcription elongation factor GreA"
FT                   /id="PRO_0000176964"
FT   COILED          45..74
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00105"
SQ   SEQUENCE   162 AA;  18230 MW;  F6774DA35140BD2B CRC64;
     MNTKFPITAK GFKKLEHELK HLKHVERKKI SEDIAEAREH GDLSENAEYE AAREKQAFIE
     GRIKELEDMT ARAEIIDICK LSGDNIKFGA TVTLIDDDTE EEVTYIIVGE YEADITKKRV
     SIASPIAKAL IGKSVGDFVE VTTPKGLKSY EVVTVEYKEL DL