ID   GREA_STAAC              Reviewed;         158 AA.
AC   Q5HFF2;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Transcription elongation factor GreA {ECO:0000255|HAMAP-Rule:MF_00105};
DE   AltName: Full=Transcript cleavage factor GreA {ECO:0000255|HAMAP-Rule:MF_00105};
GN   Name=greA {ECO:0000255|HAMAP-Rule:MF_00105}; OrderedLocusNames=SACOL1665;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC       elongation past template-encoded arresting sites. The arresting sites
CC       in DNA have the property of trapping a certain fraction of elongating
CC       RNA polymerases that pass through, resulting in locked ternary
CC       complexes. Cleavage of the nascent transcript by cleavage factors such
CC       as GreA or GreB allows the resumption of elongation from the new
CC       3'terminus. GreA releases sequences of 2 to 3 nucleotides.
CC       {ECO:0000255|HAMAP-Rule:MF_00105}.
CC   -!- SIMILARITY: Belongs to the GreA/GreB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00105}.
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DR   EMBL; CP000046; AAW38281.1; -; Genomic_DNA.
DR   RefSeq; WP_000431312.1; NC_002951.2.
DR   AlphaFoldDB; Q5HFF2; -.
DR   SMR; Q5HFF2; -.
DR   EnsemblBacteria; AAW38281; AAW38281; SACOL1665.
DR   KEGG; sac:SACOL1665; -.
DR   HOGENOM; CLU_101379_2_1_9; -.
DR   OMA; TWLTQEA; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.287.180; -; 1.
DR   Gene3D; 3.10.50.30; -; 1.
DR   HAMAP; MF_00105; GreA_GreB; 1.
DR   InterPro; IPR036953; GreA/GreB_C_sf.
DR   InterPro; IPR018151; TF_GreA/GreB_CS.
DR   InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR   InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR   InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR   InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR   InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR   InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR   PANTHER; PTHR30437; PTHR30437; 1.
DR   Pfam; PF01272; GreA_GreB; 1.
DR   Pfam; PF03449; GreA_GreB_N; 1.
DR   PIRSF; PIRSF006092; GreA_GreB; 1.
DR   SUPFAM; SSF46557; SSF46557; 1.
DR   TIGRFAMs; TIGR01462; greA; 1.
DR   PROSITE; PS00829; GREAB_1; 1.
DR   PROSITE; PS00830; GREAB_2; 1.
PE   3: Inferred from homology;
KW   Coiled coil; DNA-binding; Transcription; Transcription regulation.
FT   CHAIN           1..158
FT                   /note="Transcription elongation factor GreA"
FT                   /id="PRO_0000176970"
FT   COILED          4..70
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00105"
SQ   SEQUENCE   158 AA;  17743 MW;  EC3B0F0E6238A107 CRC64;
     MENQKQYPMT QEGFEKLERE LEELKTVKRP EVVEKIKVAR SFGDLSENSE YDAAKDEQGF
     IEQDIQRIEH MLRNALIIED TGDNNVVKIG KTVTFVELPG DEEESYQIVG SAESDAFNGK
     ISNESPMAKA LIGKGLDDEV RVPLPNGGEM NVKIVNIQ