GREA_STRP6
ID GREA_STRP6 Reviewed; 160 AA.
AC Q5XDQ7; P82553;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Transcription elongation factor GreA {ECO:0000255|HAMAP-Rule:MF_00105};
DE AltName: Full=Transcript cleavage factor GreA {ECO:0000255|HAMAP-Rule:MF_00105};
GN Name=greA {ECO:0000255|HAMAP-Rule:MF_00105}; OrderedLocusNames=M6_Spy0321;
OS Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=286636;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-946 / MGAS10394;
RX PubMed=15272401; DOI=10.1086/422697;
RA Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E.,
RA Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.;
RT "Progress toward characterization of the group A Streptococcus metagenome:
RT complete genome sequence of a macrolide-resistant serotype M6 strain.";
RL J. Infect. Dis. 190:727-738(2004).
RN [2]
RP PROTEIN SEQUENCE OF 5-24; 28-34; 73-91 AND 142-158, AND MASS SPECTROMETRY.
RC STRAIN=JRS4 / Serotype M6;
RA Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J.,
RA VanBogelen R.A.;
RT "Two-dimensional gel electrophoresis map of Streptococcus pyogenes
RT proteins.";
RL Submitted (MAY-2000) to UniProtKB.
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreA releases sequences of 2 to 3 nucleotides.
CC {ECO:0000255|HAMAP-Rule:MF_00105}.
CC -!- MASS SPECTROMETRY: Mass=19659.39; Method=Electrospray;
CC Evidence={ECO:0000269|Ref.2};
CC -!- SIMILARITY: Belongs to the GreA/GreB family. {ECO:0000255|HAMAP-
CC Rule:MF_00105}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT86456.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000003; AAT86456.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_002990993.1; NC_006086.1.
DR AlphaFoldDB; Q5XDQ7; -.
DR SMR; Q5XDQ7; -.
DR EnsemblBacteria; AAT86456; AAT86456; M6_Spy0321.
DR GeneID; 57852124; -.
DR KEGG; spa:M6_Spy0321; -.
DR HOGENOM; CLU_101379_2_1_9; -.
DR Proteomes; UP000001167; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.287.180; -; 1.
DR Gene3D; 3.10.50.30; -; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR PANTHER; PTHR30437; PTHR30437; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR PIRSF; PIRSF006092; GreA_GreB; 1.
DR SUPFAM; SSF46557; SSF46557; 1.
DR TIGRFAMs; TIGR01462; greA; 1.
DR PROSITE; PS00829; GREAB_1; 1.
DR PROSITE; PS00830; GREAB_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Direct protein sequencing; DNA-binding; Transcription;
KW Transcription regulation.
FT CHAIN 1..160
FT /note="Transcription elongation factor GreA"
FT /id="PRO_0000176983"
FT COILED 1..71
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00105"
SQ SEQUENCE 160 AA; 17653 MW; AD7C3F981B259E5C CRC64;
MAEKTYPMTL TEKEQLEKEL EELKLVRRPE IVERIKIARS YGDLSENSEY DAAKDEQAFV
EGQISTLETK IRYAEIIDSD AVAKDEVAIG KTVIVQEVGT TDKDTYHIVG AAGADIFSGK
ISNESPIAQA LIGKKTGDKV RIESPAATYD VEIISVEKTN