ID   3SA12_DABRR             Reviewed;          20 AA.
AC   P0DMV6;
DT   27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT   27-MAY-2015, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=7.2 kDa cytotoxin RVV-7 {ECO:0000303|PubMed:16997639, ECO:0000303|PubMed:17499831};
DE   Flags: Fragment;
OS   Daboia russelii (Russel's viper) (Vipera russelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Viperinae; Daboia.
OX   NCBI_TaxID=8707;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=16997639; DOI=10.1016/j.jchromb.2006.08.038;
RA   Maity G., Mandal S., Chatterjee A., Bhattacharyya D.;
RT   "Purification and characterization of a low molecular weight
RT   multifunctional cytotoxic phospholipase A2 from Russell's viper venom.";
RL   J. Chromatogr. B 845:232-243(2007).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-15, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=17499831; DOI=10.1016/j.toxicon.2007.03.018;
RA   Mandal S., Bhattacharyya D.;
RT   "Ability of a small, basic protein isolated from Russell's viper venom
RT   (Daboia russelli russelli) to induce renal tubular necrosis in mice.";
RL   Toxicon 50:236-250(2007).
CC   -!- FUNCTION: This three-finger cytotoxin shows cytotoxicity and direct
CC       nephrotoxicity. The cytotoxicity has been observed on B16F10 melanoma
CC       cells (EC(50)=2.56 uM) (PubMed:16997639) and on kidney proximal tubular
CC       epithelium LLCPK1 cells (EC(50)=4.79 uM); it is due to necrotic cell
CC       death and not to apoptosis. Direct nephrotoxicity has been deduced from
CC       binding to LLCPK1 cell line and to kidney membranes. In addition, after
CC       intravenous injection into mice tail vein, the toxin principally
CC       accumulates in kidney, but only minimally in blood, liver and brain
CC       (PubMed:17499831). {ECO:0000269|PubMed:16997639,
CC       ECO:0000269|PubMed:17499831}.
CC   -!- SUBUNIT: Monomer. Homodimerizes during storage at 30 degrees Celsius
CC       (observed after 3 days). {ECO:0000269|PubMed:16997639}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16997639}. Target
CC       cell membrane {ECO:0000250|UniProtKB:P60301}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7201.0; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16997639, ECO:0000269|PubMed:17499831};
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type IA cytotoxin sub-subfamily. {ECO:0000305}.
CC   -!- CAUTION: Surprinsingly, this toxin has also been observed to have
CC       phospholipase A2 and trypsin inhibitory activities.
CC       {ECO:0000269|PubMed:16997639}.
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DR   AlphaFoldDB; P0DMV6; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Membrane; Secreted; Target cell membrane;
KW   Target membrane; Toxin.
FT   CHAIN           1..>20
FT                   /note="7.2 kDa cytotoxin RVV-7"
FT                   /evidence="ECO:0000269|PubMed:16997639"
FT                   /id="PRO_0000433034"
FT   UNSURE          14
FT                   /note="Assigned by comparison with orthologs"
FT                   /evidence="ECO:0000305"
FT   NON_TER         20
SQ   SEQUENCE   20 AA;  2179 MW;  1FFF0FE2DE91BE85 CRC64;
     LECNKLVDIA YITCPAGKNL