GREB_ECOLI
ID GREB_ECOLI Reviewed; 158 AA.
AC P30128; P78114; Q2M771;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 2.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Transcription elongation factor GreB {ECO:0000255|HAMAP-Rule:MF_00930};
DE AltName: Full=Transcript cleavage factor GreB {ECO:0000255|HAMAP-Rule:MF_00930};
GN Name=greB {ECO:0000255|HAMAP-Rule:MF_00930};
GN OrderedLocusNames=b3406, JW3369;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15, AND FUNCTION.
RX PubMed=8431948; DOI=10.1016/0092-8674(93)90121-6;
RA Borukhov S., Sagitov V., Goldfarb A.;
RT "Transcript cleavage factors from E. coli.";
RL Cell 72:459-466(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreB releases sequences of up to 9 nucleotides in length.
CC {ECO:0000255|HAMAP-Rule:MF_00930, ECO:0000269|PubMed:8431948}.
CC -!- SIMILARITY: Belongs to the GreA/GreB family. GreB subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00930}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA58203.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U81522; AAB39763.1; -; Genomic_DNA.
DR EMBL; U18997; AAA58203.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76431.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77885.1; -; Genomic_DNA.
DR PIR; A65136; A65136.
DR RefSeq; WP_000856737.1; NZ_SSZK01000008.1.
DR RefSeq; YP_026216.2; NC_000913.3.
DR PDB; 2P4V; X-ray; 2.60 A; A/B/C/D/E/F=1-158.
DR PDB; 6RI7; EM; 3.90 A; F/G=1-158.
DR PDB; 6RIN; EM; 3.70 A; F=1-158.
DR PDBsum; 2P4V; -.
DR PDBsum; 6RI7; -.
DR PDBsum; 6RIN; -.
DR AlphaFoldDB; P30128; -.
DR SMR; P30128; -.
DR BioGRID; 4261264; 21.
DR DIP; DIP-9834N; -.
DR IntAct; P30128; 24.
DR STRING; 511145.b3406; -.
DR jPOST; P30128; -.
DR PaxDb; P30128; -.
DR PRIDE; P30128; -.
DR EnsemblBacteria; AAC76431; AAC76431; b3406.
DR EnsemblBacteria; BAE77885; BAE77885; BAE77885.
DR GeneID; 2847706; -.
DR GeneID; 58460225; -.
DR KEGG; ecj:JW3369; -.
DR KEGG; eco:b3406; -.
DR PATRIC; fig|1411691.4.peg.3323; -.
DR EchoBASE; EB1538; -.
DR eggNOG; COG0782; Bacteria.
DR HOGENOM; CLU_101379_3_0_6; -.
DR InParanoid; P30128; -.
DR OMA; DQIFFGA; -.
DR PhylomeDB; P30128; -.
DR BioCyc; EcoCyc:EG11578-MON; -.
DR EvolutionaryTrace; P30128; -.
DR PRO; PR:P30128; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0006354; P:DNA-templated transcription, elongation; IBA:GO_Central.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IDA:EcoCyc.
DR GO; GO:0031439; P:positive regulation of mRNA cleavage; IMP:EcoCyc.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IDA:EcoCyc.
DR Gene3D; 1.10.287.180; -; 1.
DR Gene3D; 3.10.50.30; -; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR HAMAP; MF_00930; GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR InterPro; IPR006358; Tscrpt_elong_fac_GreB.
DR PANTHER; PTHR30437; PTHR30437; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR PIRSF; PIRSF006092; GreA_GreB; 1.
DR SUPFAM; SSF46557; SSF46557; 1.
DR TIGRFAMs; TIGR01461; greB; 1.
DR PROSITE; PS00829; GREAB_1; 1.
DR PROSITE; PS00830; GREAB_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA-binding; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..158
FT /note="Transcription elongation factor GreB"
FT /id="PRO_0000176922"
FT HELIX 8..23
FT /evidence="ECO:0007829|PDB:2P4V"
FT HELIX 25..39
FT /evidence="ECO:0007829|PDB:2P4V"
FT TURN 42..44
FT /evidence="ECO:0007829|PDB:2P4V"
FT HELIX 46..71
FT /evidence="ECO:0007829|PDB:2P4V"
FT STRAND 79..81
FT /evidence="ECO:0007829|PDB:2P4V"
FT STRAND 86..88
FT /evidence="ECO:0007829|PDB:2P4V"
FT STRAND 90..94
FT /evidence="ECO:0007829|PDB:2P4V"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:2P4V"
FT STRAND 113..115
FT /evidence="ECO:0007829|PDB:2P4V"
FT HELIX 123..127
FT /evidence="ECO:0007829|PDB:2P4V"
FT STRAND 136..140
FT /evidence="ECO:0007829|PDB:2P4V"
FT STRAND 145..154
FT /evidence="ECO:0007829|PDB:2P4V"
SQ SEQUENCE 158 AA; 18527 MW; 28B46455FABA59CF CRC64;
MKTPLVTREG YEKLKQELNY LWREERPEVT KKVTWAASLG DRSENADYQY NKKRLREIDR
RVRYLTKCLE NLKIVDYSPQ QEGKVFFGAW VEIENDDGVT HRFRIVGYDE IFGRKDYISI
DSPMARALLK KEVGDLAVVN TPAGEASWYV NAIEYVKP
