GREB_PSESM
ID GREB_PSESM Reviewed; 157 AA.
AC Q883T5;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Transcription elongation factor GreB {ECO:0000255|HAMAP-Rule:MF_00930};
DE AltName: Full=Transcript cleavage factor GreB {ECO:0000255|HAMAP-Rule:MF_00930};
GN Name=greB {ECO:0000255|HAMAP-Rule:MF_00930}; OrderedLocusNames=PSPTO_2265;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreB releases sequences of up to 9 nucleotides in length.
CC {ECO:0000255|HAMAP-Rule:MF_00930}.
CC -!- SIMILARITY: Belongs to the GreA/GreB family. GreB subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00930}.
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DR EMBL; AE016853; AAO55779.1; -; Genomic_DNA.
DR RefSeq; NP_792084.1; NC_004578.1.
DR RefSeq; WP_005617338.1; NC_004578.1.
DR AlphaFoldDB; Q883T5; -.
DR SMR; Q883T5; -.
DR STRING; 223283.PSPTO_2265; -.
DR EnsemblBacteria; AAO55779; AAO55779; PSPTO_2265.
DR GeneID; 61789097; -.
DR KEGG; pst:PSPTO_2265; -.
DR PATRIC; fig|223283.9.peg.2299; -.
DR eggNOG; COG0782; Bacteria.
DR HOGENOM; CLU_101379_3_0_6; -.
DR OMA; DQIFFGA; -.
DR OrthoDB; 1591985at2; -.
DR PhylomeDB; Q883T5; -.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.287.180; -; 1.
DR Gene3D; 3.10.50.30; -; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR HAMAP; MF_00930; GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR InterPro; IPR006358; Tscrpt_elong_fac_GreB.
DR PANTHER; PTHR30437; PTHR30437; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR PIRSF; PIRSF006092; GreA_GreB; 1.
DR SUPFAM; SSF46557; SSF46557; 1.
DR TIGRFAMs; TIGR01461; greB; 1.
DR PROSITE; PS00829; GREAB_1; 1.
DR PROSITE; PS00830; GREAB_2; 1.
PE 3: Inferred from homology;
KW Coiled coil; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..157
FT /note="Transcription elongation factor GreB"
FT /id="PRO_0000176957"
FT COILED 52..73
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00930"
SQ SEQUENCE 157 AA; 18546 MW; 579E8BA6ECA3FEF7 CRC64;
MSTKIITQGG HEALKKELDY LWREHRPDIT QKVAWAASLG DRSENADYQY NKKLLREIDR
RVRYLRKRLE DMRVVQYSPE QEGRVFFGAW VEIENEAGDL KKFRVVGYDE IYGRNDYISI
DSPMARALLK KEVGDEVLVN TPEGEKLWFV NSIVYER