ID   GREM1_XENLA             Reviewed;         182 AA.
AC   O73754;
DT   12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Gremlin-1;
DE   AltName: Full=Cysteine knot superfamily 1, BMP antagonist 1;
DE   Flags: Precursor;
GN   Name=grem1; Synonyms=cktsf1b1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC   TISSUE=Ovary;
RX   PubMed=9660951; DOI=10.1016/s1097-2765(00)80067-2;
RA   Hsu D.R., Economides A.N., Wang X., Eimon P.M., Harland R.M.;
RT   "The Xenopus dorsalizing factor Gremlin identifies a novel family of
RT   secreted proteins that antagonize BMP activities.";
RL   Mol. Cell 1:673-683(1998).
CC   -!- FUNCTION: Cytokine that has an axial patterning activity. Acts like BMP
CC       antagonist in embryonic explants. Blocks the BMP2 activity.
CC       {ECO:0000269|PubMed:9660951}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: First detected at tailbud stages. At stage 27,
CC       appears in the pronephric duct, trunk and tail bud. In stage 30-40
CC       embryos, expression extends rostrally and caudally to include neural
CC       crest cells at all axial levels. {ECO:0000269|PubMed:9660951}.
CC   -!- SIMILARITY: Belongs to the DAN family. {ECO:0000305}.
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DR   EMBL; AF045798; AAC41279.1; -; mRNA.
DR   RefSeq; NP_001083746.1; NM_001090277.1.
DR   AlphaFoldDB; O73754; -.
DR   SMR; O73754; -.
DR   GeneID; 399094; -.
DR   KEGG; xla:399094; -.
DR   CTD; 399094; -.
DR   Xenbase; XB-GENE-865098; grem1.L.
DR   OMA; MPRTRCA; -.
DR   OrthoDB; 1270652at2759; -.
DR   Proteomes; UP000186698; Chromosome 8L.
DR   Bgee; 399094; Expressed in stomach and 16 other tissues.
DR   GO; GO:0005615; C:extracellular space; IDA:BHF-UCL.
DR   GO; GO:0036122; F:BMP binding; IDA:BHF-UCL.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0016015; F:morphogen activity; IDA:BHF-UCL.
DR   GO; GO:0048263; P:determination of dorsal identity; IMP:BHF-UCL.
DR   GO; GO:0007399; P:nervous system development; IMP:BHF-UCL.
DR   GO; GO:0014032; P:neural crest cell development; IEP:BHF-UCL.
DR   GO; GO:0014029; P:neural crest formation; IEP:BHF-UCL.
DR   GO; GO:0039022; P:pronephric duct development; IEP:BHF-UCL.
DR   GO; GO:0035582; P:sequestering of BMP in extracellular matrix; IDA:BHF-UCL.
DR   GO; GO:0032525; P:somite rostral/caudal axis specification; IMP:BHF-UCL.
DR   Gene3D; 2.10.90.10; -; 1.
DR   InterPro; IPR006207; Cys_knot_C.
DR   InterPro; IPR029034; Cystine-knot_cytokine.
DR   InterPro; IPR004133; DAN.
DR   InterPro; IPR017159; Gremlin-1/2.
DR   Pfam; PF03045; DAN; 1.
DR   PIRSF; PIRSF037254; Gremlin_precursor; 1.
DR   SMART; SM00041; CT; 1.
PE   2: Evidence at transcript level;
KW   Cytokine; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..182
FT                   /note="Gremlin-1"
FT                   /id="PRO_0000006719"
FT   DOMAIN          92..182
FT                   /note="CTCK"
FT   REGION          23..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        44
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        92..142
FT                   /evidence="ECO:0000250|UniProtKB:O60565"
FT   DISULFID        106..156
FT                   /evidence="ECO:0000250|UniProtKB:O60565"
FT   DISULFID        116..174
FT                   /evidence="ECO:0000250|UniProtKB:O60565"
FT   DISULFID        120..176
FT                   /evidence="ECO:0000250|UniProtKB:O60565"
SQ   SEQUENCE   182 AA;  20374 MW;  4EF3A655FFA1B7CE CRC64;
     MNCLVYALGS LFLLSGLLLP SSEGKKKVSG SQGAIPPPDK GQPNDSEQGQ AQPGDRVRGK
     GKGQALAAEE VLESSQEALH VTERKYLKRD WCKTQPLKQT IHEDGCNSRT IINRFCYGQC
     NSFYIPRHIR REEGSFQSCS FCKPKKFTTM VVTLNCPELQ PPTKKKRITR VKQCRCISID
     LD