GRF1_ARATH
ID GRF1_ARATH Reviewed; 530 AA.
AC O81001;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Growth-regulating factor 1;
DE Short=AtGRF1;
DE AltName: Full=Transcription activator GRF1;
GN Name=GRF1; OrderedLocusNames=At2g22840; ORFNames=T20K9.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=12974814; DOI=10.1046/j.1365-313x.2003.01862.x;
RA Kim J.H., Choi D., Kende H.;
RT "The AtGRF family of putative transcription factors is involved in leaf and
RT cotyledon growth in Arabidopsis.";
RL Plant J. 36:94-104(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION.
RX PubMed=15200956; DOI=10.1016/j.molcel.2004.05.027;
RA Jones-Rhoades M.W., Bartel D.P.;
RT "Computational identification of plant microRNAs and their targets,
RT including a stress-induced miRNA.";
RL Mol. Cell 14:787-799(2004).
RN [6]
RP FUNCTION, AND INTERACTION WITH GIF1 AND GIF2.
RX PubMed=15326298; DOI=10.1073/pnas.0405450101;
RA Kim J.H., Kende H.;
RT "A transcriptional coactivator, AtGIF1, is involved in regulating leaf
RT growth and morphology in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:13374-13379(2004).
RN [7]
RP INDUCTION.
RX PubMed=19453503; DOI=10.1111/j.1399-3054.2009.01229.x;
RA Liu D., Song Y., Chen Z., Yu D.;
RT "Ectopic expression of miR396 suppresses GRF target gene expression and
RT alters leaf growth in Arabidopsis.";
RL Physiol. Plantarum 136:223-236(2009).
RN [8]
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=20023165; DOI=10.1242/dev.043067;
RA Rodriguez R.E., Mecchia M.A., Debernardi J.M., Schommer C., Weigel D.,
RA Palatnik J.F.;
RT "Control of cell proliferation in Arabidopsis thaliana by microRNA
RT miR396.";
RL Development 137:103-112(2010).
RN [9]
RP FUNCTION, AND INDUCTION.
RX PubMed=22419826; DOI=10.1104/pp.112.193649;
RA Hewezi T., Maier T.R., Nettleton D., Baum T.J.;
RT "The Arabidopsis microRNA396-GRF1/GRF3 regulatory module acts as a
RT developmental regulator in the reprogramming of root cells during cyst
RT nematode infection.";
RL Plant Physiol. 159:321-335(2012).
RN [10]
RP FUNCTION.
RX PubMed=22751317; DOI=10.4161/psb.20420;
RA Hewezi T., Baum T.J.;
RT "Complex feedback regulations govern the expression of miRNA396 and its GRF
RT target genes.";
RL Plant Signal. Behav. 7:749-751(2012).
CC -!- FUNCTION: Transcription activator that plays a role in the regulation
CC of cell expansion in leaf and cotyledons tissues. Component of a
CC network formed by miR396, the GRFs and their interacting factors (GIFs)
CC acting in the regulation of meristem function, at least partially
CC through the control of cell proliferation. microRNA396-GRF1/GRF3
CC regulatory module acts as a developmental regulator in the
CC reprogramming of root cells during cyst nematode infection, leading to
CC the formation of the syncytium. {ECO:0000269|PubMed:12974814,
CC ECO:0000269|PubMed:15326298, ECO:0000269|PubMed:22419826,
CC ECO:0000269|PubMed:22751317}.
CC -!- SUBUNIT: Interacts with GIF1 and GIF2. {ECO:0000269|PubMed:15326298}.
CC -!- INTERACTION:
CC O81001; Q8L8A5: GIF1; NbExp=6; IntAct=EBI-1396842, EBI-1396623;
CC O81001; Q9MAL9: GIF2; NbExp=4; IntAct=EBI-1396842, EBI-1396863;
CC O81001; Q93VH6: GIF3; NbExp=3; IntAct=EBI-1396842, EBI-15194507;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU01002}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in actively growing and
CC developing tissues, such as roots, upper stems, and shoot tips
CC containing the shoot apical meristem (SAM) and flower buds. Also
CC expressed in mature flowers, but weakly expressed in mature stems and
CC leaves. {ECO:0000269|PubMed:12974814}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the early stages of leaf
CC development and expression decreases with the maturation of the leaf.
CC {ECO:0000269|PubMed:20023165}.
CC -!- INDUCTION: microRNA 396 (miR396a or miR396b) negatively regulates
CC growth-regulating factors (GRF1-4 and GRF7-9). Up-regulated in response
CC to cyst nematode infection. {ECO:0000269|PubMed:15200956,
CC ECO:0000269|PubMed:19453503, ECO:0000269|PubMed:20023165,
CC ECO:0000269|PubMed:22419826}.
CC -!- DOMAIN: The QLQ domain and WRC domain may be involved in protein-
CC protein interaction and DNA-binding, respectively.
CC -!- MISCELLANEOUS: Overexpression mutant display larger leaves and
CC cotyledons, as well as a delayed bolting of the inflorescence stem when
CC compared to wild-type plants.
CC -!- SIMILARITY: Belongs to the GRF family. {ECO:0000305}.
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DR EMBL; AY102634; AAM52876.1; -; mRNA.
DR EMBL; AC004786; AAC32431.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07363.1; -; Genomic_DNA.
DR EMBL; AB493560; BAH30398.1; -; mRNA.
DR PIR; E84617; E84617.
DR RefSeq; NP_179869.1; NM_127849.4.
DR AlphaFoldDB; O81001; -.
DR BioGRID; 2168; 20.
DR IntAct; O81001; 20.
DR STRING; 3702.AT2G22840.1; -.
DR iPTMnet; O81001; -.
DR PaxDb; O81001; -.
DR PRIDE; O81001; -.
DR ProteomicsDB; 248483; -.
DR EnsemblPlants; AT2G22840.1; AT2G22840.1; AT2G22840.
DR GeneID; 816815; -.
DR Gramene; AT2G22840.1; AT2G22840.1; AT2G22840.
DR KEGG; ath:AT2G22840; -.
DR Araport; AT2G22840; -.
DR TAIR; locus:2059206; AT2G22840.
DR eggNOG; ENOG502QRK7; Eukaryota.
DR HOGENOM; CLU_038207_0_0_1; -.
DR InParanoid; O81001; -.
DR OMA; WGWGTFH; -.
DR OrthoDB; 740672at2759; -.
DR PhylomeDB; O81001; -.
DR PRO; PR:O81001; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O81001; baseline and differential.
DR Genevisible; O81001; AT.
DR GO; GO:0005634; C:nucleus; ISS:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0019760; P:glucosinolate metabolic process; IMP:TAIR.
DR GO; GO:0061062; P:regulation of nematode larval development; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR GO; GO:0009739; P:response to gibberellin; IEP:TAIR.
DR GO; GO:0009624; P:response to nematode; IEP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR014978; Gln-Leu-Gln_QLQ.
DR InterPro; IPR031137; GRF.
DR InterPro; IPR014977; WRC_dom.
DR PANTHER; PTHR31602; PTHR31602; 1.
DR Pfam; PF08880; QLQ; 1.
DR Pfam; PF08879; WRC; 1.
DR SMART; SM00951; QLQ; 1.
DR PROSITE; PS51666; QLQ; 1.
DR PROSITE; PS51667; WRC; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..530
FT /note="Growth-regulating factor 1"
FT /id="PRO_0000419292"
FT DOMAIN 133..168
FT /note="QLQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01001"
FT DOMAIN 196..240
FT /note="WRC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 223..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 485..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 201..211
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 229..236
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT COMPBIAS 11..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 485..506
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 530 AA; 56401 MW; 6E478B781BDA301D CRC64;
MDLGVRVSGH ETVSSPGQTE LGSGFSNKQE RSGFDGEDCW RSSKLSRTST DGFSSSPASA
KTLSFHQGIP LLRSTTINDP RKGQEHMLSF SSASGKSDVS PYLQYCRNSG YGLGGMMNTS
NMHGNLLTGV KGPFSLTQWA ELEQQALIYK YITANVPVPS SLLLSLKKSF FPYGSLPPNS
FGWGSFHLGF SGGNMDPEPG RCRRTDGKKW RCSRDAVPDQ KYCERHINRG RHRSRKPVEG
QNGHNTNAAA AASAAAASTA AAVSKAAAGT SAVAMRGSDN NNSLAAAVGT QHHTNNQSTD
SLANRVQNSR GASVFPATMN LQSKETHPKQ SNNPFEFGLI SSDSLLNPSH KQASYATSSK
GFGSYLDFGN QAKHAGNHNN VDSWPEELKS DWTQLSMSIP MAPSSPVQDK LALSPLRLSR
EFDPAIHMGL GVNTEFLDPG KKTNNWIPIS WGNNNSMGGP LGEVLNSTTN SPKFGSSPTG
VLQKSTFGSL SNSSSASSTI IGDNNNKNGD GKDPLGPTTL MNTSATAPSL
