GRF2_ARATH
ID GRF2_ARATH Reviewed; 535 AA.
AC Q8L8A8; Q9T063;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Growth-regulating factor 2;
DE Short=AtGRF2;
DE AltName: Full=Transcription activator GRF2;
GN Name=GRF2; OrderedLocusNames=At4g37740; ORFNames=T28I19.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=12974814; DOI=10.1046/j.1365-313x.2003.01862.x;
RA Kim J.H., Choi D., Kende H.;
RT "The AtGRF family of putative transcription factors is involved in leaf and
RT cotyledon growth in Arabidopsis.";
RL Plant J. 36:94-104(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION.
RX PubMed=15200956; DOI=10.1016/j.molcel.2004.05.027;
RA Jones-Rhoades M.W., Bartel D.P.;
RT "Computational identification of plant microRNAs and their targets,
RT including a stress-induced miRNA.";
RL Mol. Cell 14:787-799(2004).
RN [7]
RP FUNCTION, AND INTERACTION WITH GIF1.
RX PubMed=15326298; DOI=10.1073/pnas.0405450101;
RA Kim J.H., Kende H.;
RT "A transcriptional coactivator, AtGIF1, is involved in regulating leaf
RT growth and morphology in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:13374-13379(2004).
RN [8]
RP INDUCTION.
RX PubMed=19453503; DOI=10.1111/j.1399-3054.2009.01229.x;
RA Liu D., Song Y., Chen Z., Yu D.;
RT "Ectopic expression of miR396 suppresses GRF target gene expression and
RT alters leaf growth in Arabidopsis.";
RL Physiol. Plantarum 136:223-236(2009).
RN [9]
RP FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=20023165; DOI=10.1242/dev.043067;
RA Rodriguez R.E., Mecchia M.A., Debernardi J.M., Schommer C., Weigel D.,
RA Palatnik J.F.;
RT "Control of cell proliferation in Arabidopsis thaliana by microRNA
RT miR396.";
RL Development 137:103-112(2010).
RN [10]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=21036927; DOI=10.1093/jxb/erq307;
RA Wang L., Gu X., Xu D., Wang W., Wang H., Zeng M., Chang Z., Huang H.,
RA Cui X.;
RT "miR396-targeted AtGRF transcription factors are required for coordination
RT of cell division and differentiation during leaf development in
RT Arabidopsis.";
RL J. Exp. Bot. 62:761-773(2011).
CC -!- FUNCTION: Transcription activator that plays a role in the regulation
CC of cell expansion in leaf and cotyledons tissues. Component of a
CC network formed by miR396, the GRFs and their interacting factors (GIFs)
CC acting in the regulation of meristem function, at least partially
CC through the control of cell proliferation.
CC {ECO:0000269|PubMed:12974814, ECO:0000269|PubMed:15326298,
CC ECO:0000269|PubMed:20023165, ECO:0000269|PubMed:21036927}.
CC -!- SUBUNIT: Interacts with GIF1. {ECO:0000269|PubMed:15326298}.
CC -!- INTERACTION:
CC Q8L8A8; Q8L8A5: GIF1; NbExp=4; IntAct=EBI-1396893, EBI-1396623;
CC Q8L8A8; Q9MAL9: GIF2; NbExp=3; IntAct=EBI-1396893, EBI-1396863;
CC Q8L8A8; Q93VH6: GIF3; NbExp=3; IntAct=EBI-1396893, EBI-15194507;
CC Q8L8A8; Q93Z00: TCP14; NbExp=3; IntAct=EBI-1396893, EBI-4424563;
CC Q8L8A8; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-1396893, EBI-4426144;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU01002}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in actively growing and
CC developing tissues, such as roots, upper stems, and shoot tips
CC containing the shoot apical meristem (SAM) and flower buds. Detected in
CC young leaf primordium. Also expressed in mature flowers, but weakly
CC expressed in mature stems and leaves. {ECO:0000269|PubMed:12974814,
CC ECO:0000269|PubMed:21036927}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the early stages of leaf
CC development and expression decreases with the maturation of the leaf.
CC {ECO:0000269|PubMed:20023165}.
CC -!- INDUCTION: microRNA 396 (miR396a or miR396b) negatively regulates
CC growth-regulating factors (GRF1-4 and GRF7-9).
CC {ECO:0000269|PubMed:15200956, ECO:0000269|PubMed:19453503,
CC ECO:0000269|PubMed:20023165}.
CC -!- DOMAIN: The QLQ domain and WRC domain may be involved in protein-
CC protein interaction and DNA-binding, respectively.
CC -!- MISCELLANEOUS: Overexpression mutant display larger leaves and
CC cotyledons, as well as a delayed bolting of the inflorescence stem when
CC compared to wild-type plants.
CC -!- SIMILARITY: Belongs to the GRF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB38922.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80439.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY102635; AAM52877.1; -; mRNA.
DR EMBL; AL035709; CAB38922.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161592; CAB80439.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86833.1; -; Genomic_DNA.
DR EMBL; AK176372; BAD44135.1; -; mRNA.
DR EMBL; AK176432; BAD44195.1; -; mRNA.
DR EMBL; AB493726; BAH30564.1; -; mRNA.
DR PIR; T06021; T06021.
DR RefSeq; NP_195488.2; NM_119936.5.
DR AlphaFoldDB; Q8L8A8; -.
DR BioGRID; 15211; 25.
DR IntAct; Q8L8A8; 25.
DR STRING; 3702.AT4G37740.1; -.
DR PaxDb; Q8L8A8; -.
DR PRIDE; Q8L8A8; -.
DR ProteomicsDB; 247286; -.
DR EnsemblPlants; AT4G37740.1; AT4G37740.1; AT4G37740.
DR GeneID; 829930; -.
DR Gramene; AT4G37740.1; AT4G37740.1; AT4G37740.
DR KEGG; ath:AT4G37740; -.
DR Araport; AT4G37740; -.
DR TAIR; locus:2137549; AT4G37740.
DR eggNOG; ENOG502QRK7; Eukaryota.
DR HOGENOM; CLU_038207_0_0_1; -.
DR InParanoid; Q8L8A8; -.
DR OMA; HQKHRNN; -.
DR OrthoDB; 740672at2759; -.
DR PhylomeDB; Q8L8A8; -.
DR PRO; PR:Q8L8A8; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8L8A8; baseline and differential.
DR Genevisible; Q8L8A8; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR014978; Gln-Leu-Gln_QLQ.
DR InterPro; IPR031137; GRF.
DR InterPro; IPR014977; WRC_dom.
DR PANTHER; PTHR31602; PTHR31602; 1.
DR Pfam; PF08880; QLQ; 1.
DR Pfam; PF08879; WRC; 1.
DR SMART; SM00951; QLQ; 1.
DR PROSITE; PS51666; QLQ; 1.
DR PROSITE; PS51667; WRC; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..535
FT /note="Growth-regulating factor 2"
FT /id="PRO_0000419293"
FT DOMAIN 164..199
FT /note="QLQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01001"
FT DOMAIN 227..271
FT /note="WRC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT REGION 260..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 417..437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 514..535
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 232..242
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 260..267
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT COMPBIAS 270..308
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 535 AA; 58585 MW; E3CBB15694279DDD CRC64;
MDIGVHVLGS VTSNENESLG LKELIGTKQD RSGFIGEDCL QRSLKLARTT TRAEEEENLS
SSVAAAYCKT MSFHQGIPLM RSASPLSSDS RRQEQMLSFS DKPDALDFSK YVGLDNSSNN
KNSLSPFLHQ IPPPSYFRSS GGYGSGGMMM NMSMQGNFTG VKGPFTLTQW AELEQQALIY
KYITANVPVP SSLLISIKKS FYPYGSLPPS SFGWGTFHLG FAGGNMDPEP GRCRRTDGKK
WRCSRDAVPD QKYCERHINR GRHRSRKPVE VQSGQNQTAA AASKAVTTPQ QPVVAGNTNR
SNARASSNRS LAIGSQYINP STESLPNNRG VSIYPSTVNL QPKESPVIHQ KHRNNNNPFE
FGHISSDSLL NPNTAKTYGS SFLDFSSNQE KHSGNHNHNS WPEELTSDWT QLSMSIPIAS
SSPSSTHNNN NAQEKTTLSP LRLSRELDLS IQTDETTIEP TVKKVNTWIP ISWGNSLGGP
LGEVLNSTTN SPTFGSSPTG VLQKSTFCSL SNNSSVSSPI AENNRHNGDY FHYTT
