GRF3_ARATH
ID GRF3_ARATH Reviewed; 398 AA.
AC Q9SJR5; Q8GYS6;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Growth-regulating factor 3;
DE Short=AtGRF3;
DE AltName: Full=Transcription activator GRF3;
GN Name=GRF3; OrderedLocusNames=At2g36400; ORFNames=F1O11.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=12974814; DOI=10.1046/j.1365-313x.2003.01862.x;
RA Kim J.H., Choi D., Kende H.;
RT "The AtGRF family of putative transcription factors is involved in leaf and
RT cotyledon growth in Arabidopsis.";
RL Plant J. 36:94-104(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION.
RX PubMed=15200956; DOI=10.1016/j.molcel.2004.05.027;
RA Jones-Rhoades M.W., Bartel D.P.;
RT "Computational identification of plant microRNAs and their targets,
RT including a stress-induced miRNA.";
RL Mol. Cell 14:787-799(2004).
RN [7]
RP INDUCTION.
RX PubMed=19453503; DOI=10.1111/j.1399-3054.2009.01229.x;
RA Liu D., Song Y., Chen Z., Yu D.;
RT "Ectopic expression of miR396 suppresses GRF target gene expression and
RT alters leaf growth in Arabidopsis.";
RL Physiol. Plantarum 136:223-236(2009).
RN [8]
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=20023165; DOI=10.1242/dev.043067;
RA Rodriguez R.E., Mecchia M.A., Debernardi J.M., Schommer C., Weigel D.,
RA Palatnik J.F.;
RT "Control of cell proliferation in Arabidopsis thaliana by microRNA
RT miR396.";
RL Development 137:103-112(2010).
RN [9]
RP FUNCTION, AND INDUCTION.
RX PubMed=22419826; DOI=10.1104/pp.112.193649;
RA Hewezi T., Maier T.R., Nettleton D., Baum T.J.;
RT "The Arabidopsis microRNA396-GRF1/GRF3 regulatory module acts as a
RT developmental regulator in the reprogramming of root cells during cyst
RT nematode infection.";
RL Plant Physiol. 159:321-335(2012).
CC -!- FUNCTION: Transcription activator that plays a role in the regulation
CC of cell expansion in leaf and cotyledons tissues. Component of a
CC network formed by miR396, the GRFs and their interacting factors (GIFs)
CC acting in the regulation of meristem function, at least partially
CC through the control of cell proliferation. microRNA396-GRF1/GRF3
CC regulatory module acts as a developmental regulator in the
CC reprogramming of root cells during cyst nematode infection, leading to
CC the formation of the syncytium. {ECO:0000269|PubMed:12974814,
CC ECO:0000269|PubMed:22419826}.
CC -!- INTERACTION:
CC Q9SJR5; Q93VH6: GIF3; NbExp=3; IntAct=EBI-3134219, EBI-15194507;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU01002}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in actively growing and
CC developing tissues, such as roots, upper stems, and shoot tips
CC containing the shoot apical meristem (SAM) and flower buds. Also
CC expressed in mature flowers, but weakly expressed in mature stems and
CC leaves. {ECO:0000269|PubMed:12974814}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the early stages of leaf
CC development and expression decreases with the maturation of the leaf.
CC {ECO:0000269|PubMed:20023165}.
CC -!- INDUCTION: microRNA 396 (miR396a or miR396b) negatively regulates
CC growth-regulating factors (GRF1-4 and GRF7-9). Up-regulated in response
CC to cyst nematode infection. {ECO:0000269|PubMed:15200956,
CC ECO:0000269|PubMed:19453503, ECO:0000269|PubMed:20023165,
CC ECO:0000269|PubMed:22419826}.
CC -!- DOMAIN: The QLQ domain and WRC domain may be involved in protein-
CC protein interaction and DNA-binding, respectively.
CC -!- SIMILARITY: Belongs to the GRF family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY102636; AAM52878.1; -; mRNA.
DR EMBL; AC006919; AAD24624.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09248.1; -; Genomic_DNA.
DR EMBL; AK117417; BAC42083.1; -; mRNA.
DR EMBL; BT026472; ABH04579.1; -; mRNA.
DR PIR; C84780; C84780.
DR RefSeq; NP_181181.1; NM_129197.4.
DR AlphaFoldDB; Q9SJR5; -.
DR BioGRID; 3557; 9.
DR IntAct; Q9SJR5; 9.
DR STRING; 3702.AT2G36400.1; -.
DR iPTMnet; Q9SJR5; -.
DR PaxDb; Q9SJR5; -.
DR PRIDE; Q9SJR5; -.
DR ProteomicsDB; 247035; -.
DR EnsemblPlants; AT2G36400.1; AT2G36400.1; AT2G36400.
DR GeneID; 818213; -.
DR Gramene; AT2G36400.1; AT2G36400.1; AT2G36400.
DR KEGG; ath:AT2G36400; -.
DR Araport; AT2G36400; -.
DR TAIR; locus:2044873; AT2G36400.
DR eggNOG; ENOG502QTJ4; Eukaryota.
DR HOGENOM; CLU_037902_1_0_1; -.
DR InParanoid; Q9SJR5; -.
DR OMA; RFPRMGS; -.
DR OrthoDB; 932651at2759; -.
DR PhylomeDB; Q9SJR5; -.
DR PRO; PR:Q9SJR5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJR5; baseline and differential.
DR Genevisible; Q9SJR5; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:TAIR.
DR GO; GO:0061062; P:regulation of nematode larval development; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR GO; GO:0009739; P:response to gibberellin; IEP:TAIR.
DR GO; GO:0009624; P:response to nematode; IEP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR014978; Gln-Leu-Gln_QLQ.
DR InterPro; IPR031137; GRF.
DR InterPro; IPR014977; WRC_dom.
DR PANTHER; PTHR31602; PTHR31602; 1.
DR Pfam; PF08880; QLQ; 1.
DR Pfam; PF08879; WRC; 1.
DR SMART; SM00951; QLQ; 1.
DR PROSITE; PS51666; QLQ; 1.
DR PROSITE; PS51667; WRC; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..398
FT /note="Growth-regulating factor 3"
FT /id="PRO_0000419294"
FT DOMAIN 76..111
FT /note="QLQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01001"
FT DOMAIN 144..188
FT /note="WRC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..398
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 149..159
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 177..184
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT COMPBIAS 1..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..360
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..398
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 13
FT /note="Q -> R (in Ref. 4; BAC42083)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 398 AA; 43707 MW; 2A6E15497305AF74 CRC64;
MDLQLKQWRS QQQQQHQTES EEQPSAAKIP KHVFDQIHSH TATSTALPLF TPEPTSSKLS
SLSPDSSSRF PKMGSFFSWA QWQELELQAL IYRYMLAGAA VPQELLLPIK KSLLHLSPSY
FLHHPLQHLP HYQPAWYLGR AAMDPEPGRC RRTDGKKWRC SRDVFAGHKY CERHMHRGRN
RSRKPVETPT TVNATATSMA SSVAAAATTT TATTTSTFAF GGGGGSEEVV GQGGSFFFSG
SSNSSSELLH LSQSCSEMKQ ESNNMNNKRP YESHIGFSNN RSDGGHILRP FFDDWPRSSL
QEADNSSSPM SSATCLSISM PGNSSSDVSL KLSTGNEEGA RSNNNGRDQQ NMSWWSGGGS
NHHHHNMGGP LAEALRSSSS SSPTSVLHQL GVSTQAFH
