GRF4_ARATH
ID GRF4_ARATH Reviewed; 380 AA.
AC Q8L8A7; Q9LFA0;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Growth-regulating factor 4;
DE Short=AtGRF4;
DE AltName: Full=Transcription activator GRF4;
GN Name=GRF4; OrderedLocusNames=At3g52910; ORFNames=F8J2_80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, AND TISSUE
RP SPECIFICITY.
RX PubMed=12974814; DOI=10.1046/j.1365-313x.2003.01862.x;
RA Kim J.H., Choi D., Kende H.;
RT "The AtGRF family of putative transcription factors is involved in leaf and
RT cotyledon growth in Arabidopsis.";
RL Plant J. 36:94-104(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP INDUCTION.
RX PubMed=15200956; DOI=10.1016/j.molcel.2004.05.027;
RA Jones-Rhoades M.W., Bartel D.P.;
RT "Computational identification of plant microRNAs and their targets,
RT including a stress-induced miRNA.";
RL Mol. Cell 14:787-799(2004).
RN [5]
RP INDUCTION.
RX PubMed=19453503; DOI=10.1111/j.1399-3054.2009.01229.x;
RA Liu D., Song Y., Chen Z., Yu D.;
RT "Ectopic expression of miR396 suppresses GRF target gene expression and
RT alters leaf growth in Arabidopsis.";
RL Physiol. Plantarum 136:223-236(2009).
RN [6]
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=20023165; DOI=10.1242/dev.043067;
RA Rodriguez R.E., Mecchia M.A., Debernardi J.M., Schommer C., Weigel D.,
RA Palatnik J.F.;
RT "Control of cell proliferation in Arabidopsis thaliana by microRNA
RT miR396.";
RL Development 137:103-112(2010).
CC -!- FUNCTION: Transcription activator that plays a role in the regulation
CC of cell expansion in leaf and cotyledons tissues. Component of a
CC network formed by miR396, the GRFs and their interacting factors (GIFs)
CC acting in the regulation of meristem function, at least partially
CC through the control of cell proliferation. {ECO:0000250}.
CC -!- INTERACTION:
CC Q8L8A7; Q8L8A5: GIF1; NbExp=3; IntAct=EBI-1396671, EBI-1396623;
CC Q8L8A7; Q9MAL9: GIF2; NbExp=3; IntAct=EBI-1396671, EBI-1396863;
CC Q8L8A7; Q93VH6: GIF3; NbExp=4; IntAct=EBI-1396671, EBI-15194507;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU01002}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in actively growing and
CC developing tissues, such as roots, upper stems, and shoot tips
CC containing the shoot apical meristem (SAM) and flower buds. Also
CC expressed in mature flowers, but weakly expressed in mature stems and
CC leaves. {ECO:0000269|PubMed:12974814}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the early stages of leaf
CC development and expression decreases with the maturation of the leaf.
CC {ECO:0000269|PubMed:20023165}.
CC -!- INDUCTION: microRNA 396 (miR396a or miR396b) negatively regulates
CC growth-regulating factors (GRF1-4 and GRF7-9).
CC {ECO:0000269|PubMed:15200956, ECO:0000269|PubMed:19453503,
CC ECO:0000269|PubMed:20023165}.
CC -!- DOMAIN: The QLQ domain and WRC domain may be involved in protein-
CC protein interaction and DNA-binding, respectively.
CC -!- SIMILARITY: Belongs to the GRF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB86895.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY102637; AAM52879.1; -; mRNA.
DR EMBL; AL132969; CAB86895.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79009.1; -; Genomic_DNA.
DR PIR; T47548; T47548.
DR RefSeq; NP_190859.2; NM_115151.3.
DR AlphaFoldDB; Q8L8A7; -.
DR BioGRID; 9774; 13.
DR IntAct; Q8L8A7; 13.
DR STRING; 3702.AT3G52910.1; -.
DR PaxDb; Q8L8A7; -.
DR PRIDE; Q8L8A7; -.
DR ProteomicsDB; 247036; -.
DR EnsemblPlants; AT3G52910.1; AT3G52910.1; AT3G52910.
DR GeneID; 824457; -.
DR Gramene; AT3G52910.1; AT3G52910.1; AT3G52910.
DR KEGG; ath:AT3G52910; -.
DR Araport; AT3G52910; -.
DR TAIR; locus:2085221; AT3G52910.
DR eggNOG; ENOG502QTJ4; Eukaryota.
DR HOGENOM; CLU_037902_1_0_1; -.
DR InParanoid; Q8L8A7; -.
DR OMA; EANMRDS; -.
DR OrthoDB; 932651at2759; -.
DR PhylomeDB; Q8L8A7; -.
DR PRO; PR:Q8L8A7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8L8A7; baseline and differential.
DR GO; GO:0005634; C:nucleus; ISS:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0048366; P:leaf development; TAS:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR014978; Gln-Leu-Gln_QLQ.
DR InterPro; IPR031137; GRF.
DR InterPro; IPR014977; WRC_dom.
DR PANTHER; PTHR31602; PTHR31602; 1.
DR Pfam; PF08880; QLQ; 1.
DR Pfam; PF08879; WRC; 1.
DR SMART; SM00951; QLQ; 1.
DR PROSITE; PS51666; QLQ; 1.
DR PROSITE; PS51667; WRC; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..380
FT /note="Growth-regulating factor 4"
FT /id="PRO_0000419295"
FT DOMAIN 82..117
FT /note="QLQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01001"
FT DOMAIN 151..195
FT /note="WRC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 222..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 284..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 156..166
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 184..191
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT COMPBIAS 222..268
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 284..317
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 380 AA; 42534 MW; AFFFB791CCBE68D1 CRC64;
MDLQLKQWRS QQQNESEEQG SAATKISNFF FDQIQSQTAT SAAAAPLPLF VPEPTSSSSF
SCFSPDSSNS SSSSRFLKMG NFFSWAQWQE LELQALIYRY MLAGASVPQE LLLPIKKSLL
HQSPMHFLHH PLQHSFPHHQ PSWYWGRGAM DPEPGRCKRT DGKKWRCSRD VVAGHKYCDR
HIHRGRNRSR KPVETATTTI TTTATTTASS FVLGEELGHG PNNNHFFSSG SSQPLHLSHQ
QSCSSEMKQE SNNNKRPYEA NSGFSNGRSD DGHILRHFFD DWPRSSDSTS SPMSSSTCHL
SISMPGNNTS SDVSLKLSTG NEEEEENMRN NNNEREQMNW WSNGGNHHNN MGGPLAEALR
SASSTSSVLH QMGISTQVFH
