GRF8_ARATH
ID GRF8_ARATH Reviewed; 493 AA.
AC Q9SU44; O22976;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Growth-regulating factor 8;
DE Short=AtGRF8;
DE AltName: Full=Transcription activator GRF8;
GN Name=GRF8; OrderedLocusNames=At4g24150; ORFNames=T19F6.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=12974814; DOI=10.1046/j.1365-313x.2003.01862.x;
RA Kim J.H., Choi D., Kende H.;
RT "The AtGRF family of putative transcription factors is involved in leaf and
RT cotyledon growth in Arabidopsis.";
RL Plant J. 36:94-104(2003).
RN [4]
RP INDUCTION.
RX PubMed=15200956; DOI=10.1016/j.molcel.2004.05.027;
RA Jones-Rhoades M.W., Bartel D.P.;
RT "Computational identification of plant microRNAs and their targets,
RT including a stress-induced miRNA.";
RL Mol. Cell 14:787-799(2004).
RN [5]
RP INDUCTION.
RX PubMed=19453503; DOI=10.1111/j.1399-3054.2009.01229.x;
RA Liu D., Song Y., Chen Z., Yu D.;
RT "Ectopic expression of miR396 suppresses GRF target gene expression and
RT alters leaf growth in Arabidopsis.";
RL Physiol. Plantarum 136:223-236(2009).
CC -!- FUNCTION: Transcription activator that plays a role in the regulation
CC of cell expansion in leaf and cotyledons tissues. Component of a
CC network formed by miR396, the GRFs and their interacting factors (GIFs)
CC acting in the regulation of meristem function, at least partially
CC through the control of cell proliferation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU01002}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in shoot tips and flowers.
CC {ECO:0000269|PubMed:12974814}.
CC -!- INDUCTION: microRNA 396 (miR396a or miR396b) negatively regulates
CC growth-regulating factors (GRF1-4 and GRF7-9).
CC {ECO:0000269|PubMed:15200956, ECO:0000269|PubMed:19453503}.
CC -!- DOMAIN: The QLQ domain and WRC domain may be involved in protein-
CC protein interaction and DNA-binding, respectively.
CC -!- SIMILARITY: Belongs to the GRF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB63610.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002343; AAB63610.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL109619; CAB51658.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84855.1; -; Genomic_DNA.
DR PIR; T13463; T13463.
DR RefSeq; NP_001329477.1; NM_001341645.1.
DR RefSeq; NP_194146.1; NM_118547.2.
DR AlphaFoldDB; Q9SU44; -.
DR BioGRID; 13804; 3.
DR STRING; 3702.AT4G24150.1; -.
DR PaxDb; Q9SU44; -.
DR PRIDE; Q9SU44; -.
DR ProteomicsDB; 247288; -.
DR EnsemblPlants; AT4G24150.1; AT4G24150.1; AT4G24150.
DR GeneID; 828515; -.
DR Gramene; AT4G24150.1; AT4G24150.1; AT4G24150.
DR KEGG; ath:AT4G24150; -.
DR Araport; AT4G24150; -.
DR TAIR; locus:2134986; AT4G24150.
DR eggNOG; ENOG502QVQ2; Eukaryota.
DR HOGENOM; CLU_636719_0_0_1; -.
DR InParanoid; Q9SU44; -.
DR OMA; MFMAPSS; -.
DR OrthoDB; 1073461at2759; -.
DR PhylomeDB; Q9SU44; -.
DR PRO; PR:Q9SU44; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SU44; baseline and differential.
DR Genevisible; Q9SU44; AT.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; ISS:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0048366; P:leaf development; TAS:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR014978; Gln-Leu-Gln_QLQ.
DR InterPro; IPR031137; GRF.
DR InterPro; IPR014977; WRC_dom.
DR PANTHER; PTHR31602; PTHR31602; 2.
DR Pfam; PF08880; QLQ; 1.
DR Pfam; PF08879; WRC; 1.
DR SMART; SM00951; QLQ; 1.
DR PROSITE; PS51666; QLQ; 1.
DR PROSITE; PS51667; WRC; 1.
PE 2: Evidence at transcript level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..493
FT /note="Growth-regulating factor 8"
FT /id="PRO_0000419299"
FT DOMAIN 149..184
FT /note="QLQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01001"
FT DOMAIN 243..287
FT /note="WRC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT REGION 270..302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 248..258
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 276..283
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT COMPBIAS 284..300
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 493 AA; 54609 MW; 135916AF25FB2E88 CRC64;
MRMLLGIPYV DKSVLSNSVL ERGKQDKSKL LLVDKCHYEL DVEERKEDFV GGFGFGVVEN
SHKDVMVLPH HHYYPSYSSP SSSSLCYCSA GVSDPMFSVS SNQAYTSSHS GMFTPAGSGS
AAVTVADPFF SLSSSGEMRR SMNEDAGAAF SEAQWHELER QRNIYKYMMA SVPVPPELLT
PFPKNHQSNT NPDVDTYRSG MFSIYADYKN LPLSMWMTVT VAVATGGSLQ LGIASSASNN
TADLEPWRCK RTDGKKWRCS RNVIPDQKYC ERHTHKSRPR SRKHVESSHQ SSHHNDIRTA
KNDTSQLVRT YPQFYGQPIS QIPVLSTLPS ASSPYDHHRG LRWFTKEDDA IGTLNPETQE
AVQLKVGSSR ELKRGFDYDL NFRQKEPIVD QSFGALQGLL SLNQTPQHNQ ETRQFVVEGK
QDEAMGSSLT LSMAGGGMEE TEGTNQHQWV SHEGPSWLYS TTPGGPLAEA LCLGVSNNPS
SSTTTSSCSR SSS
